CircNF1-419 improves the gut microbiome structure and function in AD-like mice

Abstract

Our pre-experiments found that the brain circRNA sequence profiles and gut microbiota in AD-like mice were changed, as circNF1-419 could enhance autophagy to ameliorate senile dementia in AD-like mice, so we conclude that there might some connections between circRNA and gut microbiome. Therefore, we use the over-expressed circNF1-419 adeno-associated virus (AAV) animal system with the aim of identifying possible connections. Our results showed that over-expression of circNF1-419 in brain not only influenced the cholinergic system of brain, but also changed the gut microbiota composition as the Candidatus Arthromitus, Lachnospiraceae FCS020 group, Lachnospiraceae UCG-006, and [Eubacterium] xylanophilum group, and the intestinal homeostasis and physiology, and even the gut microbiota trajectory in new born mice. These findings demonstrate a link between circRNA and gut microbiome, enlarge the 'microbiome-transcriptome' linkage library and provide more information on gut-brain axis.

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota

“…Furuya and colleagues revealed that Blautia hansenii can hydrolyze glucosylceramide to ceramide in plants 73 . Previously, we showed that circNF1-419 could regulate the synthesis and metabolism of ceramides, and the changed ceramides were enriched in three signaling pathways (neurotrophin, sphingolipid, and adipocytokine) 41 . We could conclude that the couples of Blautia and ceramide, butyrate biosynthesis bacteria, and butyrate might be one of interactions between circRNA and microbiome-gut-brain axis, but we still need much more evidence.…”

“…Previously, using in an overexpressed circNF1-419 adeno-associated virus system, we showed that circRNAs in the brain influenced the cholinergic system of the brain, and changed the GM composition, intestinal homeostasis/physiology, and even the GM trajectory in newborn mice 41 . Another system, circZCCHC11 (mmu_ circ_0001239 ), showed a “sponge” miRNA function and mediated a series of chain reactions in the brain, which then influenced gut function and GM engraftment from their parent 41 . Those data demonstrated a link between circRNAs and the GM, enlarged the microbiome–transcriptome linkage library, and provided additional information on the gut–brain axis.…”

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut–brain axis

“…ND2 ; Gastranaerophilales; Enterococcus faecalis ; Helicobacter ; [ Eubacterium ] coprostanoligenes group ; Flavobacteriaceae ; Bacteroides ; Parasutterella ; Lactobacillus ; Turicibacter ; Faecalibaculum ; [ Eubac-terium ] ventriosum group ; Candidatus Saccharimonas ; Acinetobacter ; Odoribacter ; Ruminiclostridium 5 ; Mollicutes RF9 ; Erysipelotrichaceae ; and Erysipelato-clostridium (more details are shown in Supplementary Figure 8 ). Influences of gut microbiota on brain function were shown in our previous paper [ 38 ].…”

“…Raw pyrosequencing reads obtained from the sequencer were denoised using Titanium PyroNoise software. The resulting pyrosequencing reads were analyzed by common analysis methods, as described previously [ 38 ].…”

The Emerging Functions of Circular RNAs in Bladder Cancer

“…CircSHPRH was identified to encode a 146 amino acids protein which inhibits tumor growth by stopping ubiquitin proteasome-mediated degradation of SHPRH protein in glioma [ 81 ]. In addition to regulating basic biological processes, circRNAs also play critical roles in the progression of different types of cancers [ 82 , 83 , 84 , 85 , 86 , 87 , 88 ], cardiovascular disorders [ 89 , 90 , 91 ], neuronal degenerative diseases [ 92 , 93 , 94 , 95 ], and other physiological conditions [ 96 , 97 , 98 , 99 , 100 , 101 ]. Due to the high stability of circRNAs, we believe that they can make monumental contributions to the diagnosis and treatment of diseases.…”