CircMYC Regulates Glycolysis and Cell Proliferation in Melanoma

Jin, Cheng; Dong, Dake; Yang, Zhen; Xia, Rushan; Tao, Shiqin; Piao, Meishan

doi:10.1007/s12013-019-00895-0

Cited by 34 publications

(33 citation statements)

References 37 publications

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“…Abundant evidence has demonstrated that cir-cRNAs could regulate the glycolysis of cancer cells. For example, a novel circRNA circMYC promotes the proliferation and glycolysis of human melanoma cells by directly binding to miR-1236, as a miRNA sponge, then targets LDHA to accelerate the glycolysis 16 . CircRNA ciRS-122 (hsa_circ_0005963) is positively correlated with chemoresistance of colorectal cancer, and is determined to acts as miR-122 sponge for targeting PKM2, which was delivered by the exosomes from oxaliplatin-resistant cells 17 .…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circSLC25A16 contributes to the glycolysis of non-small-cell lung cancer through epigenetic modification

Shangguan

Hong

et al. 2020

Cell Death Dis

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Growing evidence has highlighted the roles of circular RNAs (circRNAs) in non-small-cell lung cancer (NSCLC), however, their roles in NSCLC glycolysis remains poorly understood. CircRNAs microarray profiles discovered a novel exon-derived circRNA, circSLC25A16 (hsa_circ_0018534), in NSCLC tissue samples. In NSCLC samples, high-expression of circSLC25A16 was associated with unfavorable prognosis. Cellular experiments revealed that circSLC25A16 accelerated the glycolysis and proliferation of NSCLC cells. Besides, circSLC25A16 knockdown repressed the in vivo growth by xenograft assays. RNA-fluorescence in situ hybridization (RNA-FISH) illustrated that circSLC25A16 and miR-488-3p were both located in cytoplasm. Mechanistic experiments demonstrated that circSLC25A16 interacts with miR-488-3p/HIF-1α, which activates lactate dehydrogenase A (LDHA) by facilitating its transcription. Collectively, present research reveals the crucial function of circSLC25A16 on NSCLC glycolysis through miR-488-3p/HIF-1α/LDHA, suggesting the underlying pathogenesis for NSCLC and providing a therapeutic strategy for precise treatment.

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Section: Discussionmentioning

confidence: 99%

Circular RNA circSLC25A16 contributes to the glycolysis of non-small-cell lung cancer through epigenetic modification

Shangguan

Hong

et al. 2020

Cell Death Dis

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“…Several cir-cRNAs were verified to be associated with melanoma progression. For example, Jin et al manifested that cir-cMYC was elevated in melanoma and facilitated melanoma cell growth and glycolysis [19]. Luan et al verified that circ_0084043 was highly expressed in melanoma and facilitated melanoma cell proliferation and motility proved that the circ_0025039 level was raised in melanoma and its silencing hampered melanoma cell growth, colony formation, metastasis, and glycolysis by binding to miR-198 [21].…”

Section: Discussionmentioning

confidence: 99%

Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis

et al. 2020

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Background: Numerous literatures have demonstrated that circular RNAs (circRNAs) are involved in multiple types of tumors. However, the effects of circRNAs in melanoma are not very clear. In this study, we aimed to investigate the roles and mechanisms of circ-FOXM1 in melanoma. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to determine the expression of circ-FOXM1, microRNA-143-3p (miR-143-3p), and Flotillin 2 (FLOT2) mRNA. 3-(4,5-Dimethyl-2-thiazolyl)-2,5diphenyl-2-H-tetrazolium bromide (MTT) assay, flow cytometry analysis, and transwell assay were employed to test cell proliferation, apoptosis, and invasion, respectively. The glucose consumption and lactate production were examined by specific kits. Western blot assay was utilized for the detection of hexokinase2 (HK2), pyruvate kinase isozyme type M2 (PKM2), and FLOT2. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were employed to verify the targeting association between miR-143-3p and circ-FOXM1 or FLOT2. A murine xenograft model was established to explore the effect of circ-FOXM1 in vivo. Results: Circ-FOXM1 was elevated and miR-143-3p was reduced in melanoma tissues and cells. Circ-FOXM1 deficiency impeded cell proliferation, invasion, and glycolysis and facilitated cell apoptosis in melanoma in vitro and tumorigenesis in vivo. Circ-FOXM1 acted as a sponge of miR-143-3p and the impacts of circ-FOXM1 silencing on cell proliferation, apoptosis, invasion, and glycolysis were overturned by miR-143-3p deletion. Moreover, FLOT2 was a target gene of miR-143-3p and FLOT2 overexpression rescued the inhibitory effect of miR-143-3p on melanoma progression. Conclusion: Circ-FOXM1 facilitated the development of melanoma by upregulating FLOT2 through miR-143-3p.

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“…15 circMYC has been reported to be highly expressed in melanoma and promoted the proliferation of human melanoma cells. 16 There are no reports about the expression of circMYC in serum exosomes of patients with NPC and its role in prognostic evaluation. This study focused on the expression of circMYC in the serum exosomes of patients with NPC and its relationship with the recurrence and prognosis of patients with NPC.…”

Section: Introductionmentioning

confidence: 99%

“…There is a strong association between the expression of circMYC and the response to drugs 15 . circMYC has been reported to be highly expressed in melanoma and promoted the proliferation of human melanoma cells 16 . There are no reports about the expression of circMYC in serum exosomes of patients with NPC and its role in prognostic evaluation.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of exosomal circMYC in radioresistant nasopharyngeal carcinoma

Luo

Liu

et al. 2020

Head & Neck

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Background: The relationship between circulating exosomal circular RNA (circRNA) and prognosis of patients with nasopharyngeal carcinoma (NPC) remain unknown. This study focused on the expression of exosomal circMYC and its relationship with the recurrence and prognosis of patients with NPC. Methods: The circulating exosomes were obtained from 210 patients with NPC. Quantitative polymerase chain reaction, 5-ethynyl-2 0-deoxyuridine (EdU) staining, colony formation, and bioinformatic analysis were performed. Results: Circulating exosomal circMYC was significantly increased in patients with NPC and was associated with tumor size, lymph node metastasis, TNM stage, survival rate, and disease recurrence. Gain-functional and lossfunctional experiments revealed that overexpression of circMYC promoted cell proliferation and reduce radiosensitivity, while knockdown of circMYC inhibited cell proliferation and enhanced radiotherapy. Conclusion: circMYC is an oncogene in NPC cells and can enhance the radiotherapy resistance of NPC cells. Circulating exosomal circMYC can be used as a potential therapeutic target for NPC.

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CircMYC Regulates Glycolysis and Cell Proliferation in Melanoma

Cited by 34 publications

References 37 publications

Circular RNA circSLC25A16 contributes to the glycolysis of non-small-cell lung cancer through epigenetic modification

Circular RNA circSLC25A16 contributes to the glycolysis of non-small-cell lung cancer through epigenetic modification

Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis

Diagnostic value of exosomal circMYC in radioresistant nasopharyngeal carcinoma

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