Growing evidence has highlighted the roles of circular RNAs (circRNAs) in non-small-cell lung cancer (NSCLC), however, their roles in NSCLC glycolysis remains poorly understood. CircRNAs microarray profiles discovered a novel exon-derived circRNA, circSLC25A16 (hsa_circ_0018534), in NSCLC tissue samples. In NSCLC samples, high-expression of circSLC25A16 was associated with unfavorable prognosis. Cellular experiments revealed that circSLC25A16 accelerated the glycolysis and proliferation of NSCLC cells. Besides, circSLC25A16 knockdown repressed the in vivo growth by xenograft assays. RNA-fluorescence in situ hybridization (RNA-FISH) illustrated that circSLC25A16 and miR-488-3p were both located in cytoplasm. Mechanistic experiments demonstrated that circSLC25A16 interacts with miR-488-3p/HIF-1α, which activates lactate dehydrogenase A (LDHA) by facilitating its transcription. Collectively, present research reveals the crucial function of circSLC25A16 on NSCLC glycolysis through miR-488-3p/HIF-1α/LDHA, suggesting the underlying pathogenesis for NSCLC and providing a therapeutic strategy for precise treatment.
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The torque load spectrum is an important basis for the strength design and durability test verification of tractor power take-off (PTO), and the performance and reliability of tractor PTO directly affect the quality and efficiency of agricultural operations. In this paper, taking the PTO torque load as the object, a PTO loading method based on the dynamic load spectrum acquired in the actual field work was proposed in this paper. Based on the Peak Over Threshold model, the extrapolation of the PTO load spectrum was realized, and the load spectrum throughout the whole life cycle was obtained. On the basis of this, the mobile tractor PTO loading test bench and Fuzzy-Proportional-Integral-Derivative (Fuzzy-PID) controller were developed to achieve the dynamic loading of the PTO load spectrum, and the dynamic characteristics were analyzed and verified by the simulation and laboratory test. The results showed that with the time domain extrapolation method, the load extreme value was expanded from (63.24, 469.50) to (60.88, 475.18), and the coverage was expanded by 1.98%. By comparing with the fitting results, statistical characteristics and rain flow counting results, the load spectrum extrapolation method was effective. In addition, the response time of simulation and laboratory test were 0.05s and 0.75s, respectively; the maximum error was 1.77% and 4.03%, respectively; and the goodness of fit was 16.78 N·m, which indicated that the PTO loading test bench, can accurately restore the dynamic loading of the tractor and the Fuzzy-PID controller had better accuracy and stability. It would provide a reference for the practical application of PTO load spectrum of the tractors.
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