Circadian Rhythms of Melatonin, Cortisol, and Clock Gene Expression During Simulated Night Shift Work

James, Francine O.; Cermakian, Nicolas; Boivin, Diane B.

doi:10.1093/sleep/30.11.1427

Cited by 117 publications

(120 citation statements)

References 47 publications

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“…We demonstrate that, following night shifts, the relative phase of the rhythms of cytokine secretion and immune cell counts is disturbed, and these two rhythms no longer demonstrate the conventional phase relationship with the sleep-wake cycle and with the external environment. Thus, night shift leads to a disruption of the rhythmicity of the immune system, probably as a consequence of the desynchronization between the central and peripheral clocks, as we described previously when comparing central markers (e.g., cortisol and melatonin rhythms) with peripheral markers (e.g., clock gene expression in PBMCs) (35,39,40). Such a disruption might play a role in the increased incidence of various medical conditions in shift workers, such as the increased risk for infections, autoimmune diseases, cancer, and cardiovascular and metabolic disorders (2,(8)(9)(10)(11)(12)(13).…”

Section: Discussionmentioning

confidence: 74%

“…Subjects were admitted to a time-free laboratory environment for 6 d ( Fig. 1) (40). Following the first day (experimental day 1), they underwent a procedure called constant posture (CP) for 24 h (experimental day 2), involving minimal levels of activity (subject had to stay in a semirecumbent position), dim light exposure, and hourly isocaloric snacks.…”

Section: Subjects and Experimental Protocolmentioning

confidence: 99%

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Simulated Night Shift Disrupts Circadian Rhythms of Immune Functions in Humans

Cuesta

Boudreau

Dubeau-Laramée

et al. 2016

The Journal of Immunology

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107

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Recent research unveiled a circadian regulation of the immune system in rodents, yet little is known about rhythms of immune functions in humans and how they are affected by circadian disruption. In this study, we assessed rhythms of cytokine secretion by immune cells and tested their response to simulated night shifts. PBMCs were collected from nine participants kept in constant posture over 24 h under a day-oriented schedule (baseline) and after 3 d under a night-oriented schedule. Monocytes and T lymphocytes were stimulated with LPS and PHA, respectively. At baseline, a bimodal rhythmic secretion was detected for IL-1β, IL-6, and TNF-α: a night peak was primarily due to a higher responsiveness of monocytes, and a day peak was partly due to a higher proportion of monocytes. A rhythmic release was also observed for IL-2 and IFN-γ, with a nighttime peak due to a higher cell count and responsiveness of T lymphocytes. Following night shifts, with the exception of IL-2, cytokine secretion was still rhythmic but with peak levels phase advanced by 4.5–6 h, whereas the rhythm in monocyte and T lymphocyte numbers was not shifted. This suggests distinct mechanisms of regulation between responsiveness to stimuli and cell numbers of the human immune system. Under a night-oriented schedule, only cytokine release was partly shifted in response to the change in the sleep–wake cycle. This led to a desynchronization of rhythmic immune parameters, which might contribute to the increased risk for infection, autoimmune diseases, cardiovascular and metabolic disorders, and cancer reported in shift workers.

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Section: Discussionmentioning

confidence: 74%

Section: Subjects and Experimental Protocolmentioning

confidence: 99%

Simulated Night Shift Disrupts Circadian Rhythms of Immune Functions in Humans

Cuesta

Boudreau

Dubeau-Laramée

et al. 2016

The Journal of Immunology

Self Cite

107

View full text Add to dashboard Cite

show abstract

“…Further, we used a whole population of white blood cells, which includes mononuclear and polymorphonuclear cells, as it was found that there is no difference in the expression of PER1 mRNA between these two populations of white blood cells (16). However, it should be pointed out that different rhythmicity of clock gene expression was observed for peripheral blood leukocytes, probably due to the presence of various cell populations in that tissue (14)(15)(16)(17)(18)(19)(20)(21)(22). In the majority of in vivo studies, daily monitoring of the expression of clock genes in human peripheral leukocyte populations has been extensively investigated.…”

Section: Discussionmentioning

confidence: 99%

“…In leukocytes, including peripheral blood mononuclear cells (PBMC), the acrophase of PER messenger ribonucleic acid (mRNA) was observed in the early morning and morning (13)(14)(15)(16)(17)(18)(19). CRY1 and CRY2 mRNA expression level peaked also in the morning in circulating leukocytes (20), while the positive regulator of circadian rhythm -BMAL1 mRNA -presented maximal expression in PBMC and leukocytes in the evening or midnight, as it is in approximate antiphase with the other negative regulators of circadian rhythm (15,18,(20)(21)(22). Interestingly, in the majority of studies, various populations of blood leukocytes presented lack of rhythmicity of CLOCK mRNA expression (17,20).…”

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confidence: 99%

Circadian gene expression in peripheral blood leukocytes of rotating night shift nurses

Reszka¹,

Pepłońska²,

Wieczorek³

et al. 2012

Scand J Work Environ Health

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“…Individual differences in adaptation to rotational-shift work have been reported, and several studies have examined the effects of this adaptation on circadian phase, sleep quality, work performance, and the endocrine system in rotational-shift workers [16][17][18][19][20][21]. In addition, differences in adaptation and sleep status have been reported in nurses who work night shift [22,23], and the severity of sleep problems during adaptation was attributed to accumulative subjective fatigue [22].…”

Section: Introductionmentioning

confidence: 99%

Association Between Sleep Quality and Subjective Fatigue in Night-Shift Nurses with Good and Poor Sleep

et al. 2018

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Purpose This study aimed to explore the association between sleep quality and subjective fatigue in nurses who performed rotational-shift work. Methods A cross-sectional survey was conducted and included nurses working day and night shifts (n = 35) at Toyama University Hospital in Japan. To examine the influence of sleep quality on subjective fatigue, participants were categorized as poor (n = 23) or good (n = 12) sleepers. They assessed their own sleep status using the St Mary's Hospital Sleep Questionnaire and subjective fatigue following shift changes (holiday to day shift, day shift to day shift, before night shift, after night shift, and day shift to holiday). Results Poor sleepers' State-Trait Anxiety Inventory scores were higher relative to those of good sleepers. Moreover, good sleepers recovered from subjective fatigue after sleep following all shift changes. Poor sleepers' subjective fatigue also reduced after sleep following 'before night shift', 'after night shift', and 'day shift to holiday' shift changes but not 'holiday to day shift' or 'day shift to day shift' shift changes. Sleep duration was stable across shift changes in good sleepers, and poor sleepers' sleep durations were significantly longer following 'before night shift', 'after night shift', and 'day shift to holiday' shift changes relative to those following 'holiday to day shift' and 'day shift to day shift' shift changes. Conclusions The results suggested that poor sleepers' subjective fatigue following 'before night shift', 'after night shift', and 'day shift to holiday' could be reduced by increasing sleep duration, indicating that it is important to ensure sufficient sleep duration to improve all nurses' sleep quality and reduce poor sleepers' subjective fatigue. Furthermore, sufficient relaxation at bedtime and the reduction of anxiety are important in maintaining good sleep quality.

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Circadian Rhythms of Melatonin, Cortisol, and Clock Gene Expression During Simulated Night Shift Work

Cited by 117 publications

References 47 publications

Simulated Night Shift Disrupts Circadian Rhythms of Immune Functions in Humans

Simulated Night Shift Disrupts Circadian Rhythms of Immune Functions in Humans

Circadian gene expression in peripheral blood leukocytes of rotating night shift nurses

Association Between Sleep Quality and Subjective Fatigue in Night-Shift Nurses with Good and Poor Sleep

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