Circadian gene expression in peripheral blood leukocytes of rotating night shift nurses

Reszka, Edyta; Pepłońska, Beata; Wieczorek, Edyta; Sobala, Wojciech; Bukowska, Agnieszka; Gromadzińska, Jolanta; Lie, Jenny-Anne; Kjuus, Helge; Wąsowicz, Wojciech

doi:10.5271/sjweh.3303

Cited by 24 publications

(13 citation statements)

References 30 publications

(48 reference statements)

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“…Overall, our results from oral mucosa cells suggest that shift work brings about a general disruption in clock gene expression. This is consistent with other studies that described in 1 case an increase of BMAL1, PER2, and REV-ERBa expression, and in the other case, a reduction of PER3 expression in rotational-shift-working nurses compared with day-working nurses (23,42). However, these studies had data for blood sampled at only 1 time point, which limited their interpretation.…”

Section: Discussionsupporting

confidence: 90%

Disruption of central and peripheral circadian clocks in police officers working at night

Koshy¹,

Cuesta²,

Boudreau³

et al. 2019

FASEB j.

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Working atypical schedules leads to temporal misalignments between a worker's rest‐activity cycle and their endogenous circadian system. Several studies have reported disturbed centrally controlled rhythms, but little is known on shift workers' peripheral clocks. Here, we assessed central clock markers, urinary 6‐sulfatoxymelatonin and salivary cortisol, and clock gene expression in 2 peripheral clocks, oral mucosa cells and peripheral blood mononuclear cells (PBMCs), in 11 police officers. Before working 7 consecutive nights, officers' centrally controlled rhythms were aligned to a day‐oriented schedule. These rhythms were partially realigned to the shifted schedule and dampened after a week working nights. For peripheral clocks at baseline, Period (PER)1–3 and nuclear receptor subfamily 1, group D, member 1 (REV‐ERBα) in oral mucosa cells had a significant mRNA peak in the afternoon, whereas in PBMCs, higher PER1–3 expression was observed at 10:00 compared with 19:30. After a week working nights, PER1–3 and REV‐ERBα expression in oral mucosa cells lost rhythmicity, and in PBMCs, the morning/evening difference observed at baseline was lost. To our knowledge, this is the first study to demonstrate the disruption of several peripheral clocks in real shift workers. Molecular circadian disturbances are believed to have important clinical implications for the occurrence of shift work–associated medical disorders.—Koshy, A., Cuesta, M., Boudreau, P., Cermakian, N., Boivin, D. B. Disruption of central and peripheral circadian clocks in police officers working at night. FASEB J. 33, 6789–6800 (2019). http://www.fasebj.org

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Section: Discussionsupporting

confidence: 90%

Disruption of central and peripheral circadian clocks in police officers working at night

Koshy¹,

Cuesta²,

Boudreau³

et al. 2019

FASEB j.

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show abstract

“…Current results from the available literature on the functionality of other SNPs in these genes are not conclusive. For example, it has previously been seen that, in healthy nurses and midwives who worked either day or rotating night shifts, there were no differences between BMAL1 , CLOCK , CRY1 , CRY2 , PER1 , PER2 and PER3 gene expression levels in peripheral blood leukocytes [52]. In the same subjects, however, polymorphisms of the circadian genes BMAL1 (rs2279287), CLOCK (rs1801260), PER1 (rs2735611), PER2 (rs2304672), PER3 (rs10462020), CRY1 (rs8192440) and CRY2 (rs10838527, rs10838527) were not found to be different between shift workers and day-only workers [53].…”

Section: Discussionmentioning

confidence: 99%

Analysis of polymorphisms in the circadian-related genes and breast cancer risk in Norwegian nurses working night shifts

et al. 2013

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IntroductionSome studies have suggested that night work may be associated with an increased risk of breast cancer in nurses. We aimed to explore the role of circadian gene polymorphisms in the susceptibility to night work-related breast cancer risk.MethodsWe conducted a nested case-control study of Norwegian nurses comprising 563 breast cancer cases and 619 controls within a cohort of 49,402 Norwegian nurses ages 35 to 74 years. We studied 60 single-nucleotide polymorphisms (SNPs) in 17 genes involved in the regulation of the circadian rhythm in cases and controls. The data were analyzed in relation to the two exposure variables "maximum number of consecutive night shifts ever worked" and "maximum number of consecutive night shifts worked for at least 5 years." The odds of breast cancer associated with each SNP was calculated in the main effects analysis and in relation to night shift work. The statistically significant odds ratios were tested for noteworthiness using two Bayesian tests: false positive report probability (FPRP) and Bayesian false discovery probability (BFDP).ResultsIn the main effects analysis, CC carriers of rs4238989 and GG carriers of rs3760138 in the AANAT gene had increased risk of breast cancer, whereas TT carriers of BMAL1 rs2278749 and TT carriers of CLOCK rs3749474 had reduced risk. The associations were found to be noteworthy using both the FPRP and BFDP tests. With regard to the effect of polymorphisms and night work, several significant associations were observed. After applying FPRP and BFDP in women with at least four night shifts, an increased risk of breast cancer was associated with variant alleles of SNPs in the genes AANAT (rs3760138, rs4238989), BMAL1 (rs2290035, rs2278749, rs969485) and ROR-b (rs3750420). In women with three consecutive night shifts, a reduced risk of breast cancer was associated with carriage of variant alleles of SNPs in CLOCK (rs3749474), BMAL1 (rs2278749), BMAL2 (rs2306074), CSNK1E (rs5757037), NPAS2 (rs17024926), ROR-b (rs3903529, rs3750420), MTNR1A (rs131113549) and PER3 (rs1012477).ConclusionsSignificant and noteworthy associations between several polymorphisms in circadian genes, night work and breast cancer risk were found among nurses who had worked at least three consecutive night shifts.

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“…In a study where SW nurses were analyzed at the end of the night shift, no differences were reported in clock gene expression (72). Similarly, Reszka and co-workers (75) found no statistically significant changes of investigated circadian genes among SW nurses after adjusting for the hour of blood collection. The time of sampling should be carefully taken into account in studies analyzing peripheral levels of clock gene expression since they fluctuate naturally in a circadian fashion.…”

Section: Bracci Et Almentioning

confidence: 93%

“…To our knowledge, few studies have been performed on circadian gene mRNA expression among shift work- Clock genes, melatonin, and estradiol among shift nurses ers (72)(73)(74)(75)(76). In a study where SW nurses were analyzed at the end of the night shift, no differences were reported in clock gene expression (72).…”

Section: Bracci Et Almentioning

confidence: 99%

Rotating-shift nurses after a day off: peripheral clock gene expression, urinary melatonin, and serum 17-β-estradiol levels

Bracci¹,

Manzella²,

Copertaro³

et al. 2014

Scand J Work Environ Health

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Circadian gene expression in peripheral blood leukocytes of rotating night shift nurses

Cited by 24 publications

References 30 publications

Disruption of central and peripheral circadian clocks in police officers working at night

Disruption of central and peripheral circadian clocks in police officers working at night

Analysis of polymorphisms in the circadian-related genes and breast cancer risk in Norwegian nurses working night shifts

Rotating-shift nurses after a day off: peripheral clock gene expression, urinary melatonin, and serum 17-β-estradiol levels

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