Circadian Rhythmicity in the Sensitivity of Two Strains of Mice to Whole-Body Radiation

Pizzarello, Donald J.; Isaak, Daniel; Chua, K. E.; Rhyne, Alfred L.

doi:10.1126/science.145.3629.286

Cited by 53 publications

(10 citation statements)

References 10 publications

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“…Chronoradiosensitivity of mice and rats has been recognized since the early 1960s (Pizzarello et al 1963, Pizzarello et al 1964, Nelson 1966, Hellwig and Rosenkranz 1968, Haus et al 1974, Haus 2002), and has been suggested to be a useful guide to time delivery of radiotherapy in humans for malignancy. In rats and mice, mortality was shown to be higher when irradiation was delivered during the night time hours (Nelson 1966) and/or during the early light hours (Pizzarello et al 1964, Hellwig and Rosenkranz 1968, Haus 2002). Our findings showed the highest mortality in the morning hours and are in agreement with these studies.…”

Section: Discussionmentioning

confidence: 99%

Establishing a Murine Model of the Hematopoietic Syndrome of the Acute Radiation Syndrome

et al. 2012

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We have developed a murine model of the Hematopoietic Syndrome of the Acute Radiation Syndrome (H-ARS) for efficacy testing of medical countermeasures (MCM) against radiation according to the FDA Animal Rule. Ten to 12 week old male and female C57BL/6 mice were exposed to the LD50/30-LD70/30 dose of total body irradiation (TBI, 137Cs, 0.62-0.67 Gy min-1) in the morning hours when mice were determined to be most radiosensitive, and assessed for 30 day survival and mean survival time (MST). Antibiotics were delivered in the drinking water on days 4-30 post-TBI at a concentration based on the amount of water that lethally-irradiated mice were found to consume. The fluoroquinolones, ciprofloxacin and levofloxacin, and the tetracycline doxycycline and aminoglycoside neomycin, all significantly increased MST of decedent mice, while ciprofloxacin (p=0.061) and doxycycline + neomycin (p=0.005) showed at least some efficacy to increase 30 day survival. Blood sampling (30uL/mouse every 5th day) was found to negatively impact 30 day survival. Histopathology of tissues harvested from non-moribund mice showed expected effects of lethal irradiation, while moribund mice were largely septicemic with a preponderance of enteric organisms. Kinetics of loss and recovery of peripheral blood cells in untreated mice and those treated with two MCM, granulocyte-colony stimulating factor and Amifostine, further characterized and validated our model for use in screening studies and pivotal efficacy studies of candidate MCM for licensure to treat irradiated individuals suffering from H-ARS.

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Section: Discussionmentioning

confidence: 99%

Establishing a Murine Model of the Hematopoietic Syndrome of the Acute Radiation Syndrome

et al. 2012

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“…H e relates RUGH'S finding (2) to his own, stating that RUGH'S data do not conflict with his early postulations on cyclic influences on radiosensitivity but, on the contrary, are in good agreement with his own findings. (1,4) He also enumerates incompatibilities between the experimental design of his first study'l) and that of STRAUBE(~) in an attempt * Work performed under the auspices of the U.S.…”

Section: Introductionmentioning

confidence: 98%

Radiation Lethality and Circadian Rhythmicity in Mice

Spalding¹,

McWilliams²

1965

Health Physics

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Two methods were used to study the influence of circadian rhythmicity on radiosensitivity in mice as a factor in acute radiation effects studies. The results suggested the presence of diurnal differences in radiosensitivity ; however, these slight differences were neither consistent nor significant. It was concluded that circadian rhythmicity is not a primary factor in studying whole-body effects from acute radiation exposure.

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“…Earlier studies in the experimental laboratory had prompted the clinical studies, as they had shown a circadian change in the toxicity of whole‐body irradiation, gauged by the dose that killed 50% of the mice tested (LD 50 )(Figure 4)(25; cf. 26–28). The factors underlying the nonsinusoidality in this figure are not known.…”

Section: Introductionmentioning

confidence: 99%

“…of whole-body irradiation, gauged by the dose that killed 50% of the mice tested (LD 50 ) ( Figure 4) (25; cf. [26][27][28]. The factors under-lying the nonsinusoidality in this figure are not known.…”

Section: Introductionmentioning

confidence: 99%

Chronomics*^†: circadian and circaseptan timing of radiotherapy, drugs, calories, perhaps nutriceuticals and beyond

et al. 2003

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We suggest a putative benefit from timing nutriceuticals (substances that are both nutrients and pharmaceuticals) such as antioxidants for preventive or curative health care, based on the proven merits of timing nutrients, drugs, and other treatments, as documented, i.a., in India. The necessity of timing melatonin, a major antioxidant, is noted. A protocol to extend the scope of chronoradiotherapy awaits testing. Imaging in time by mapping rhythms and broader time structures, chronomes, for earliest diagnoses, for example detection of vascular disease risk, is recommended. The study of rhythms and broader chronomes leads to a dynamic functional genomics, guided by imaging in time of free radicals and antioxidants, amongst many other variables.

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Circadian Rhythmicity in the Sensitivity of Two Strains of Mice to Whole-Body Radiation

Abstract: When male mice of the Swiss-Webster and C(3)H strains are maintained on a light-dark cycle in which the light begins at 7 a.m. and ends at 7 p.m., they are more sensitive to whole body x-irradiation (800 to 900 roentgens) given at 2 a.m. than at any other time in the cycle tested.

Cited by 53 publications

References 10 publications

Establishing a Murine Model of the Hematopoietic Syndrome of the Acute Radiation Syndrome

Establishing a Murine Model of the Hematopoietic Syndrome of the Acute Radiation Syndrome

Radiation Lethality and Circadian Rhythmicity in Mice

Chronomics*^†: circadian and circaseptan timing of radiotherapy, drugs, calories, perhaps nutriceuticals and beyond

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Circadian Rhythmicity in the Sensitivity of Two Strains of Mice to Whole-Body Radiation

Abstract: When male mice of the Swiss-Webster and C(3)H strains are maintained on a light-dark cycle in which the light begins at 7 a.m. and ends at 7 p.m., they are more sensitive to whole body x-irradiation (800 to 900 roentgens) given at 2 a.m. than at any other time in the cycle tested.

Cited by 53 publications

References 10 publications

Establishing a Murine Model of the Hematopoietic Syndrome of the Acute Radiation Syndrome

Establishing a Murine Model of the Hematopoietic Syndrome of the Acute Radiation Syndrome

Radiation Lethality and Circadian Rhythmicity in Mice

Chronomics*†: circadian and circaseptan timing of radiotherapy, drugs, calories, perhaps nutriceuticals and beyond

Contact Info

Product

Resources

About

Chronomics*^†: circadian and circaseptan timing of radiotherapy, drugs, calories, perhaps nutriceuticals and beyond