Circadian rhythm affects the preventive role of pulsed electromagnetic fields on ovariectomy-induced osteoporosis in rats

Jing, Da; Shen, Guanghao; Huang, Jinghui; Xie, Kangning; Cai, Jing; Qian, Xu; Wu, Xiaoming; Luo, Erping

doi:10.1016/j.bone.2009.09.021

Cited by 66 publications

(71 citation statements)

References 48 publications

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“…Several experimental studies have demonstrated that PEMF stimulation could prevent bone mass loss and microarchitecture deterioration, and improve bone's biomechanical properties in various animal models of osteoporosis [16][17][18]. This efficiency of PEMF was further confirmed by numerous clinical investigations [19,20].…”

Section: Introductionmentioning

confidence: 60%

“…PEMF has proven to be an effective noninvasive method in treating several diseases both experimentally and clinically during the past decades, such as cardiovascular disease, soft tissue injury, and skeletal system disorder [18,27,31]. However, little data to date have reported the possible impact of PEMF on DM and DM-induced bone complications.…”

Section: Discussionmentioning

confidence: 99%

“…The number of turns of the central coil was 266 turns and the number of turns of the outside coils was 500 turns. This apparatus with the assembly of three coils has presented significantly upgraded magnetic field uniformity and has been described in detail in our previous studies [18]. As depicted in Fig.…”

Section: Pemf Treatmentmentioning

confidence: 99%

“…1, the PEMF waveform consisted of a pulsed burst (burst width, 5 ms; pulse width, 0.2 ms; pulse wait, 0.02 ms; burst wait, 60 ms; pulse rise and fall times, 0.3 and 2.0 μs) repeated at 15 Hz. This waveform has been proved effective in the prevention and treatment of bone disorders and cardiovascular diseases by our research group for a long period of experiment [18,27].…”

Section: Pemf Treatmentmentioning

confidence: 99%

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The preventive effects of pulsed electromagnetic fields on diabetic bone loss in streptozotocin-treated rats

et al. 2010

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Section: Introductionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Pemf Treatmentmentioning

confidence: 99%

Section: Pemf Treatmentmentioning

confidence: 99%

See 2 more Smart Citations

The preventive effects of pulsed electromagnetic fields on diabetic bone loss in streptozotocin-treated rats

et al. 2010

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“…Three months later, molars were extracted for cells culture and blood samples of the rats from two groups were collected to evaluate estrogen level. Bone mineral density (BMD) of the femurs were measured by employing a Lunar DPX-IQ Dual energy X-ray absorptiometry (DXA) (Lunar Corporation, Madison, WI, USA), which was equipped with appropriate software for bone density assessment in small laboratory animals as reported previously (Jing et al 2010). Left femurs were harvested from the carcasses, wrapped in salinesoaked gauze and stored at −20°C.…”

Section: Animals and Osteoporosis Modelmentioning

confidence: 99%

Estrogen Deficiency Leads to Impaired Osteogenic Differentiation of Periodontal Ligament Stem Cells in Rats

Zhang

Liu

Zhou

et al. 2011

Tohoku J. Exp. Med.

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Estrogen deficiency in post-menopausal women is considered as one of the risk factors for periodontal diseases. The periodontal ligament is a connective tissue that connects cementum and alveolar bone to constrain teeth within the jaw. Periodontal ligament stem cells (PDLSCs) isolated from the periodontal ligament can differentiate into many types of specialized cells, including osteoblast-like cells that can be used to regenerate alveolar bone. However, little is known about the effect of estrogen-deficient microenvironment on the osteogenic differentiation of PDLSCs. The aim of this study was to explore the role of estrogen on the potential for osteogenic differentiation of PDLSCs using a rat model of osteoporosis. Three-month-old female Sprague-Dawley rats were divided into two groups (n = 6 for each): ovariectomized (OVX) and sham-operated rats (Sham). Then the characteristics of PDLSCs isolated from these rats were investigated. Real-time PCR analysis showed the lower expression levels of estrogen receptors (ERα and ERβ) mRNAs in PDLSCs of OVX animals compared to Sham control. Mineralization assay demonstrated fewer calcium deposits in PDLSCs from OVX group than those from Sham group. Treatment with 17β-estradiol (E2) significantly enhanced the osteogenic differentiation of PDLSCs from both groups in vitro. Furthermore, by means of lentivirus-mediated siRNA targeting ERα or ERβ, the expression of ERα or ERβ was down-regulated (> 50% reduction), which impaired the estrogen-induced osteogenic differentiation of PDLSCs from both groups (> 50% reduction). These results indicate that estrogen plays an important role in maintaining osteogenic differentiation of PDLSCs, which acts through both ERα and ERβ.

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Pulsed electromagnetic fields on postmenopausal osteoporosis in southwest China: A randomized, active‐controlled clinical trial

Liu

Yang

et al. 2013

Bioelectromagnetics

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A randomized, active-controlled clinical trial was conducted to examine the effect of pulsed electromagnetic fields (PEMFs) on women with postmenopausal osteoporosis (PMO) in southwest China. Forty-four participants were randomly assigned to receive alendronate or one course of PEMFs treatment. The primary endpoint was the mean percentage change in bone mineral density of the lumbar spine (BMDL), and secondary endpoints were the mean percentage changes in left proximal femur bone mineral density (BMDF), serum 25OH vitamin D3 (25(OH)D) concentrations, total lower-extremity manual muscle test (LE MMT) score, and Berg Balance Scale (BBS) score. The BMDL, BMDF, total LE MMT score and BBS score were recorded at baseline, 5, 12, and 24 weeks. Serum concentrations of 25(OH)D were measured at baseline and 5 weeks. Using a mixed linear model, there was no significant treatment difference between the two groups in the BMDL, BMDF, total LE MMT score, and BBS score (P ≥ 0.05). For 25(OH)D concentrations, the effects were also comparable between the two groups (P ≥ 0.05) with the Mann-Whitney's U-test. These results suggested that a course of PEMFs treatment with specific parameters was as effective as alendronate in treating PMO within 24 weeks.

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Circadian rhythm affects the preventive role of pulsed electromagnetic fields on ovariectomy-induced osteoporosis in rats

Cited by 66 publications

References 48 publications

The preventive effects of pulsed electromagnetic fields on diabetic bone loss in streptozotocin-treated rats

The preventive effects of pulsed electromagnetic fields on diabetic bone loss in streptozotocin-treated rats

Estrogen Deficiency Leads to Impaired Osteogenic Differentiation of Periodontal Ligament Stem Cells in Rats

Pulsed electromagnetic fields on postmenopausal osteoporosis in southwest China: A randomized, active‐controlled clinical trial

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