The preventive effects of pulsed electromagnetic fields on diabetic bone loss in streptozotocin-treated rats

Jing, Da; Cai, Jing; Shen, Guanghao; Huang, Jinghui; Li, F.; Li, J.; Lu, Linhe; Luo, Erping; Qian, Xu

doi:10.1007/s00198-010-1447-3

Cited by 63 publications

(71 citation statements)

References 43 publications

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“…This mechanism may be responsible, at least in part, for the effect of magnetic fields on calcium ions in beta cells. Changes were greater, and reversibility lower, with square than with sinusoidal waves (15). A comparison of 10 Hz and 100 Hz PEMF soon after diabetic peripheral neuropathy treatment showed that the lower frequency was more effective (16).…”

Section: Discussionmentioning

confidence: 91%

Effects of pulsed electromagnetic fields on lipid peroxidation and antioxidant levels in blood and liver of diabetic rats

Gozen¹,

Demirel²,

Akan³

et al. 2018

Eur J Ther

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Objective: The present study investigated the protective effects of a pulsed electromagnetic field (PEMF) in a rat model of diabetes by analyzing oxidative/nitrosative stress parameters. Methods: Rats were randomly divided into four groups of eight each: a control group, a sham PEMF group, a diabetes group, and a diabetes+PEMF group. Diabetes was induced in the sham PEMF, diabetes, and diabetes+PEMF groups by treatment with 50 mg/ kg streptozotocin (STZ). Rats in the sham PEMF group were treated identically, without running the instrument. Following the development of diabetes, rats in the diabetes+PEMF group were treated with PEMF for 60 min/day for 4 weeks. Results: Levels of oxidants, such as malondialdehyde (MDA), nitric oxide (NO), and myeloperoxidase (MPO), and antioxidants, such as superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT), were measured in blood and liver tissue samples. MPO, MDA, and NO levels were significantly higher, and SOD levels significantly lower, in the sham PEMF and diabetes groups than in the control group (p<0.05 each), whereas the levels of all these four (i.e.: MPO, MDA, NO, and SOD) in the diabetes+PEMF group were close to those in the control group. GSH levels were significantly lower in the sham PEMF, diabetes, and diabetes+PEMF groups than in the control group (p<0.05 each), whereas CAT levels were similar in all the four groups. Conclusion: Results indicate that PEMF affects MDA, NO, MPO, SOD, and GSH levels and regulates diabetes-associated damage by reducing oxidative stress and increasing the levels of antioxidants. PEMF may be a non-invasive treatment option for diabetes and associated complications.

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Section: Discussionmentioning

confidence: 91%

Effects of pulsed electromagnetic fields on lipid peroxidation and antioxidant levels in blood and liver of diabetic rats

Gozen¹,

Demirel²,

Akan³

et al. 2018

Eur J Ther

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show abstract

“…Bassett et al for the first time found that PEMF treatment, a more accessible and affordable non-contact modality, was able to dramatically accelerate fracture healing in patients [12]. Numerous studies have further proved that PEMF could promote potently osteogenesis and enhance bone mineralization both in vivo and in vitro [15], [16], [47]. Clinical investigations revealed that PEMF produced satisfying therapeutic effects on fresh and nonunion fractures and osteoporosis [14], [17].…”

Section: Discussionmentioning

confidence: 99%

“…Basically, the three most common EMF application modalities well documented thus far include PEMF, static magnetic fields (SMF) and rotating magnetic fields (RMF). A large body of evidence has shown that PEMF displayed strong osteogenic potential both experimentally and clinically [15], [16], [17], [18], which was primarily associated with the induction of electric currents in tissues to initiate a battery of biological cascades. Several investigators also reported that SMF exposure was able to stimulate in vivo skeletal anabolic responses and increase BMD [19], [20].…”

Section: Introductionmentioning

confidence: 99%

Moderate-Intensity Rotating Magnetic Fields Do Not Affect Bone Quality and Bone Remodeling in Hindlimb Suspended Rats

Jing

Cai

et al. 2014

PLoS ONE

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Abundant evidence has substantiated the positive effects of pulsed electromagnetic fields (PEMF) and static magnetic fields (SMF) on inhibiting osteopenia and promoting fracture healing. However, the osteogenic potential of rotating magnetic fields (RMF), another common electromagnetic application modality, remains poorly characterized thus far, although numerous commercial RMF treatment devices have been available on the market. Herein the impacts of RMF on osteoporotic bone microarchitecture, bone strength and bone metabolism were systematically investigated in hindlimb-unloaded (HU) rats. Thirty two 3-month-old male Sprague-Dawley rats were randomly assigned to the Control (n = 10), HU (n = 10) and HU with RMF exposure (HU+RMF, n = 12) groups. Rats in the HU+RMF group were subjected to daily 2-hour exposure to moderate-intensity RMF (ranging from 0.60 T to 0.38 T) at 7 Hz for 4 weeks. HU caused significant decreases in body mass and soleus muscle mass of rats, which were not obviously altered by RMF. Three-point bending test showed that the mechanical properties of femurs in HU rats, including maximum load, stiffness, energy absorption and elastic modulus were not markedly affected by RMF. µCT analysis demonstrated that 4-week RMF did not significantly prevent HU-induced deterioration of femoral trabecular and cortical bone microarchitecture. Serum biochemical analysis showed that RMF did not significantly change HU-induced decrease in serum bone formation markers and increase in bone resorption markers. Bone histomorphometric analysis further confirmed that RMF showed no impacts on bone remodeling in HU rats, as evidenced by unchanged mineral apposition rate, bone formation rate, osteoblast numbers and osteoclast numbers in cancellous bone. Together, our findings reveal that RMF do not significantly affect bone microstructure, bone mechanical strength and bone remodeling in HU-induced disuse osteoporotic rats. Our study indicates potentially obvious waveform-dependent effects of electromagnetic fields-stimulated osteogenesis, suggesting that RMF, at least in the present form, might not be an optimal modality for inhibiting disuse osteopenia/osteoporosis.

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“…It has reported that the cellular functions of bone marrow stromal cell [13], osteoblast [14,15], and osteoclast [16,17] are abnormal under diabetic conditions. Recent study has shown that PEMFs can partially prevent deterioration of bone strength and architecture in streptozotocin (STZ)-induced diabetic rats [18]. However, the precise mechanisms accounting for these favorable biological responses are unclear.…”

Section: Introductionmentioning

confidence: 98%

Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

Zhou

Liao

et al. 2014

Endocrine

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Evidences have shown that pulsed electromagnetic fields (PEMFs) can partially prevent bone loss in streptozotocin (STZ)-induced diabetic rats. However, the precise mechanisms accounting for these favorable effects are unclear. This study aimed to investigate the effects of PEMFs on bone mass and receptor activator of nuclear factor κB ligand (RANKL)/osteoprotegerin (OPG) and Wnt/β-catenin signaling pathway in STZ rats. Thirty 3-month-old Sprague Dawley rats were randomly divided into the following three groups (n = 10): control group (injection of saline vehicle), DM group (injection of STZ), and PEMFs group (injection of STZ + PEMFs exposure). One week following injection of STZ, rats in the PEMFs group were subject to PEMFs stimulus for 40 min/day, 5 days/week, and lasted for 12 weeks. After 12 week intervention, the results showed that PEMFs increased serum bone-specific alkaline phosphatase level and bone mineral density, and inhibited deterioration of bone microarchitecture and strength in STZ rats. Furthermore, PEMFs up-regulated the mRNA expressions of low-density lipoprotein receptor-related protein 5, β-catenin and runt-related gene 2 (Runx2), and down-regulated dickkopf1 in STZ rats. However, mRNA expressions of RANKL and OPG were not affected by PEMFs. PEMFs can prevent the diabetes-induced bone loss and reverse the deterioration of bone microarchitecture and strength by restoring Runx2 expression through regulation of Wnt/β-catenin signaling, regardless of its no glucose lowering effect.

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The preventive effects of pulsed electromagnetic fields on diabetic bone loss in streptozotocin-treated rats

Abstract: The results demonstrated that PEMF could partially prevent DM-induced bone strength and architecture deterioration and improve the impaired bone formation. PEMF might become a potential additive method for inhibiting diabetic osteoporosis.

Cited by 63 publications

References 43 publications

Effects of pulsed electromagnetic fields on lipid peroxidation and antioxidant levels in blood and liver of diabetic rats

Effects of pulsed electromagnetic fields on lipid peroxidation and antioxidant levels in blood and liver of diabetic rats

Moderate-Intensity Rotating Magnetic Fields Do Not Affect Bone Quality and Bone Remodeling in Hindlimb Suspended Rats

Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

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