Circadian Changes in Electrolyte Concentration in Plasma and Erythrocytes in Two-week-old Calves

Abstract

Skotnicka E., W. F. Skrzypczak, M. Ozgo: Circadian Changes in Electrolyte Concentrations in Plasma and Erythrocytes of Two-week-old Calves. Acta vet. Brno 1997, 66: 141-146.The aim of this study was to determine changes in the content of Na, K, CI, Ca, Mg and P in the plasma and erythrocytes of 14-day-old calves with simultaneously performed evaluation of the occurrence and analysis of the rhythms.The studies were carried out on a group of 10 Black and White, clinically healthy female calves aged 14 days. Seven days before and during the experiment, the animals were kept in uniform environmental conditions (LD = 16:8).Blood samples were collected seven times over a period of 24 hours, spaced by 4 hour intervals, i. e., at 9:00, 13:00, 17:00, 21 :00, 1:00,5:00 and 9:00. Wbole blood and plasma were used in the analysis. In the whole blood, hematocrit and concentrations of Na, K, CI, Ca, Mg and P were determined. The plasma osmolality was also determined. The concentrations of sodium, potassium and chlorides in the erythrocytes were estimated using an indirect method.In the present study, the circadian variations in plasma concentrations of sodium. potassium and chlorides and in its osmolality were found in two-week-old female calves. Changes in plasma content of sodium and potassium within twenty-four hours were significant (P<O.Ol). On the other hand, calcium, magnesium and phosphorus concentrations in plasma and concentrations of sodium, potassium and chlOlides in erythrocytes were stable over the twenty-four hour period under study.

A review of the renal system and diurnal variations of renal activity in livestock

“…Muszczynski [35] and Skotnicka [48], in goats, and Skotnicka et al [44], in calves, did not observe diurnal changes in plasma osmolality. However Skotnicka [48], in a study on pregnant goats, observed significant plasma osmolality differences between maximum (in the evening) and minimum (in the morning).…”

Diurnal Variation of Plasma Arginine-vasopressin in Pregnant and Non-pregnant Goats (Capra hircus)

“…Skotnicka et al (1997) in calves, did not observed diurnally changes of plasma osmolality. However, Kanervo et al (1991) and Windle et al (1992) observed circadian changes of plasma osmolality in rats, with the highest values around midnight, in the middle of animals' activity period.…”

“…In the present study, plasma Cl -concentration in nonpregnant and pregnant goats remained relatively stable. Similarly Muszczyƒski et al (1996) in pregnant goats and Skotnicka et al (1997) in calves did not find any diurnal variations in Cl -plasma concentrations.…”

“…In the present study, plasma Na + concentration in the non-pregnant and pregnant goats was relatively stable during the 24 h period similar to results by Muszczyƒski et al (1996) in pregnant goats. Yet Kokkonen et al (2001) in anoestrous goats, Skotnicka et al (1997) in calves and Kanabrocki et al (1973) and Sothern et al (1996) in man observed circadian variations in plasma sodium concentration. In the present study, no diurnal variation in plasma K + concentration in both groups of goats was found, similar to that by Kokkonen et al (2001) in anoestrous goats.…”

“…In the present study, no diurnal variation in plasma K + concentration in both groups of goats was found, similar to that by Kokkonen et al (2001) in anoestrous goats. However, diurnal variations in the plasma K + concentration were observed in calves (Skotnicka et al 1997), in pregnant goats (Muszczyƒski et al 1996) and in humans (Kanabrocki et al 1973). M oreover, Solomon et al (1991) observed an increase in the plasma K + concentration during the day, with the maximum around noon.…”