Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis

Abstract

Background Circular RNAs (circRNAs) play vital roles in non‐small cell lung cancer (NSCLC) progression. Our research analyzed the role of circ_0110498 on the cisplatin (DDP) resistance of NSCLC. Methods Cell glycolysis was analyzed by measuring glucose consumption and lactate production. Protein expression was determined by western blot analysis. The expression of circ_0110498, microRNA (miR)‐1287‐5p and RBBP4 was detected by RT‐qPCR assay. Cell counting kit‐8, colony formation and transwell assays, together with flow cytometry were conducted to analyze cell DDP resistance, proliferation, metastasis and apoptosis. Results Circ_0110498 expression was elevated in DDP‐resistant NSCLC tissues and cells. Circ_0110498 silencing not only suppressed the DDP resistance of NSCLC cells by inhibiting cell growth, metastasis and glycolysis, but also enhanced the DDP sensitivity of NSCLC tumors. MiR‐1287‐5p was sponged by circ_0110498, and its inhibitor also reversed the effect of circ_0110498 silencing on the DDP resistance of NSCLC cells. MiR‐1287‐5p interacted with RBBP4, and RBBP4 overexpression partly reversed the inhibitory effect of miR‐1287‐5p on the DDP resistance of NSCLC cells. Conclusion Circ_0110498 facilitated DDP resistance partly through mediating the miR‐1287‐5p/RBBP4 signaling in NSCLC.

Interpreting the molecular mechanisms of RBBP4/7 and their roles in human diseases (Review)

“…Elevated RBBP4 expression in non-small cell LC (NSCLC) tissues amplifies cell proliferation and invasion, and is concomitantly linked to an adverse clinical outcome ( 13 , 84 ). RBBP4 is also increased in cisplatin-resistant NSCLC, affecting drug resistance ( 85 ), and is associated with enhanced DNA damage sensitivity and repair pathway activity ( 86 ). Additionally, in lung adenocarcinoma (LUAD) cells, RBBP4 interacts with chromobox homolog 3, which is found to be upregulated in current smokers with LUAD, thereby promoting LUAD progression ( 87 ).…”

“…For example, increased expression of RBBP4 has been linked to mitigating lead-induced neuronal apoptosis, suggesting a potential role in alleviating lead poisoning and related neurological disorders ( 178 ). Additionally, the interaction of RBBP4 with the efficacy of multiple drugs has been extensively studied, including its role in enhancing the sensitivity of GBM cells to TMZ ( 56 , 95 ), suppression of LC cell malignancy via ropivacaine by downregulating RBBP4 ( 179 ), and the identification of the circ-0110498/miR-1287-5p/RBBP4 axis as a novel target for overcoming cisplatin resistance in NSCLC ( 85 ). RBBP4 is also considered a potential target for treating CRC with PPD ( 119 ).…”

Non-Coding RNAs as Key Regulators in Lung Cancer

“…miR-1287-5p was identified to interact with RBBP4 , and overexpression of RBBP4 partially reversed the inhibitory effect of miR-1287-5p on DDP resistance in NSCLC cells. Their findings suggest that circ_0110498 contributes to DDP resistance in NSCLC, at least in part through mediating the miR-1287-5p/ RBBP4 signaling pathway [ 205 ].…”