Circ_0000285 regulates proliferation, migration, invasion and apoptosis of osteosarcoma by miR-409-3p/IGFBP3 axis

Abstract

Background Circular RNAs (circRNAs) are important regulators in the pathogenesis of diseases and affects the occurrence and development of diseases. However, the role of circRNAs in osteosarcoma (OS) has not been fully elucidated. Methods The expression of circ_0000285, miR-409-3p and insulin-like growth factor binding protein 3 (IGFBP3) was detected using quantitative real-time PCR (qRT-PCR). The protein level of IGFBP3 was measured using western blot. CCK-8 and colony formation assays were used to determine cell proliferation. Flow cytometry was applied to measure cell cycle and cell apoptosis. Transwell assay was used to assess cell invasion and migration. Dual-luciferase reporter assay and RNA Binding Protein Immunoprecipitation (RIP) assay were performed to determine the relationship among circ_0000285, miR-409-3p and IGFBP3. The animal experiments were performed to determine the function of circ_0000285 in vivo. Results In this study, we found that the expression of circ_0000285 was significantly increased in OS tissues and cells and was enriched in the cytoplasm. Knockdown of circ_0000285 inhibited OS growth in vitro and in vivo. Moreover, miR-409-3p was a target miRNA of circ_0000285 and miR-409-3p targets to IGFBP3 in OS. Besides, circ_0000285 could promote proliferation, migration, invasion and inhibit apoptosis of osteosarcoma by miR-409-3p/IGFBP3 axis. Conclusion In this study, circ_0000285 regulated proliferation, migration, invasion and apoptosis of OS cells by miR-409-3p/IGFBP3 axis, implying that circ_0000285 was a potential target for OS therapy.

Inhibition of circular RNA_0000285 prevents cell proliferation and induces apoptosis in thyroid cancer by sponging microRNA‑654‑3p

“…For example, Zhang et al ( 28 ) confirmed that circ_0067934 depletion accelerates cell apoptosis and represses cell proliferation, migration and invasion in thyroid cancer by sponging miR-1304 and regulating C-X-CR motif chemokine receptor 1 expression. hsa_circ_0000285 is a novel circRNA that is dysregulated in various cancers and disease types, including osteosarcoma ( 29 ), diabetic nephropathy ( 30 ) and laryngocarcinoma ( 31 ). However, the specific role and molecular mechanism of circ_0000285 in thyroid cancer have not yet been fully investigated.…”

The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

“…After being isolated from LN229 and T98G cells, total RNA was incubated with RNase R or the mock treatment for 30 min (37°C). Then, the expression of circ_0001367 and GAPDH was determined by qRT-PCR ( 24 ).…”

“…Actinomycin D (2 mg/ml, APExBIO, USA) was added to culture medium to culture LN229 and T98G cells for 24 h. Next, the cells were harvested, and their expression of circ_0001367 and GAPDH was determined by qRT-PCR ( 24 ).…”

Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the key pathways and central genes of osteosarcoma in vivo

“…Most patients with OS will eventually die of lung metastases [4] . Research results have proven that the occurrence and evolution of OS are closely related to gene mutations and expression levels [5][6][7] .…”