Circ007071 Inhibits Unsaturated Fatty Acid Synthesis by Interacting with miR-103-5p to Enhance <i>PPAR</i>γ Expression in the Dairy Goat Mammary Gland

Chen, Zhi; Lu, Qinyue; Zhang, Xinlong; Zhang, Zhiyue; Cao, Xiang; Wang, Kun; Lu, Xiaotan; Yang, Zhangping; Loor, Juan J.; Jiao, Peixin

doi:10.1021/acs.jafc.2c06174

Cited by 7 publications

(6 citation statements)

References 42 publications

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Section: Effect Of Cis-9 Trans-11 Cla On Milk Fat Synthesismentioning

confidence: 79%

“…This process is accompanied by an upregulation of transcription factors SREBP and PPARG, and downstream expression of genes related to milk fat synthesis. Taken together, cis-9, trans-11 CLA attenuates the lipopolysaccharideinduced inhibition of fatty acid synthesis by inhibiting oxidative stress and autophagy in bovine mammary epithelial cells [79]. teins LC3B, Beclin1, and ATG5 and increases in protein abundance and immunofluorescence intensity of p62, indicating the Nrf2 signaling pathway leading to a reduction in autophagy, thereby restoring cellular homeostasis (Figure 7).…”

Section: Effect Of Cis-9 Trans-11 Cla On Milk Fat Synthesismentioning

confidence: 91%

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Progress of Conjugated Linoleic Acid on Milk Fat Metabolism in Ruminants and Humans

Wang,

Xin,

Chen

et al. 2023

Animals

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As a valuable nutrient in milk, fat accounts for a significant proportion of the energy requirements of ruminants and is largely responsible for determining milk quality. Fatty acids (FAs) are a pivotal component of milk fat. Conjugated linoleic acid (CLA) is one of the naturally occurring FAs prevalent in ruminant dairy products and meat. Increasing attention has been given to CLA because of its anti-cancer, anti-inflammatory, immune regulation, and lipid metabolism regulation properties, and these benefits potentially contribute to the growth and health of infants. In breast milk, CLA is present in trace amounts, mainly in the form of cis-9, trans-11 CLA. Notably, cis-9, trans-11 CLA improves the milk fat rate while trans-10, cis-12 CLA inhibits it. Apart from having multiple physiological functions, CLA is also a pivotal factor in determining the milk quality of ruminants, especially milk fat rate. In response to growing interest in green and healthy functional foods, more and more researchers are exploring the potential of CLA to improve the production performance of animals and the nutritional value of livestock products. Taken together, it is novel and worthwhile to investigate how CLA regulates milk fat synthesis. It is the purpose of this review to clarify the necessity for studying CLA in ruminant milk fat and breast milk fat.

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Section: Effect Of Cis-9 Trans-11 Cla On Milk Fat Synthesismentioning

confidence: 79%

Section: Effect Of Cis-9 Trans-11 Cla On Milk Fat Synthesismentioning

confidence: 91%

Progress of Conjugated Linoleic Acid on Milk Fat Metabolism in Ruminants and Humans

Wang,

Xin,

Chen

et al. 2023

Animals

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“…In a different study, Chen et al (2022) [ 102 ] explored the role of circRNAs in fatty acid metabolism within the mammary glands of dairy goats. Using RNA-seq, they identified 211 DE circRNAs during early and peak lactation phases.…”

Section: Circular Rnas In Goat: Shaping Health and Productivitymentioning

confidence: 99%

Understanding Circular RNAs in Health, Welfare, and Productive Traits of Cattle, Goats, and Sheep

Kirgiafini,

Kyrgiafini,

Gournaris

et al. 2024

Animals

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Circular RNAs (circRNAs) are unique noncoding RNA molecules, notable for their covalent closed-loop structures, which play a crucial role in regulating gene expression across a variety of biological processes. This review comprehensively synthesizes the existing knowledge of circRNAs in three key livestock species: Bos taurus (cattle), Ovis aries (sheep), and Capra hircus (goats). It focuses on their functional importance and emerging potential as biomarkers for disease detection, stress response, and overall physiological health. Specifically, it delves into the expression and functionality of circRNAs in these species, paying special attention to traits critical to livestock productivity such as milk production, meat quality, muscle development, wool production, immune responses, etc. We also address the current challenges faced in circRNA research, including the need for standardized methodologies and broader studies. By providing insights into the molecular mechanisms regulated by circRNAs, this review underscores their scientific and economic relevance in the livestock industry. The potential of circRNAs to improve animal health management and the quality of animal-derived products aligns with growing consumer concerns for animal welfare and sustainability. Thus, this paper aims to guide future research directions while supporting the development of innovative strategies in livestock management and breeding.

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“…Triglyceride content was quantified using protein concentration per milligram. For specific methods, please refer to Chen et al [21,22].…”

Section: Triglycerides and Cholesterol Analysismentioning

confidence: 99%

The Molecular Mechanism of circRNA-11228/miR-103/INSIG1 Pathway Regulating Milk Fat Synthesis in Bovine Mammary Epithelial Cells

Li,

Wu,

Wang

et al. 2024

Agriculture

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Milk, known for its high content of short- and medium-chain fatty acids and unsaturated fatty acids, has attracted substantial attention due to its nutritional and health value. The regulation of fatty acid metabolism by non-coding RNAs has become a subject of growing attention, particularly in relation to fatty acid production at the transcriptional/epigenetic and post-transcriptional levels. This study established the circRNA-11228/miR-103/INSIG1 (insulin-inducible gene) regulatory network using methods such as qRT-PCR, dual luciferase reporting, and Western blot, with INSIG1 serving as the starting point. The experimental validation of circRNA-11228’s impact on cholesterol levels, lipid droplet secretion, and unsaturated fatty acid content was conducted using various assays, including triglycerides, cholesterol, oil red O, andEdU(5-ethynyl-2’-deoxyuridine) in bovine mammary epithelial cells (BMECs). Furthermore, the transfection of mimics and inhibitors synthesized from miR-103 into BMECs confirmed that miR-103 can promote cholesterol synthesis and lipid droplet secretion. Conversely, the INSIG1 gene was found to inhibit cholesterol synthesis and lipid droplet secretion. The “remediation” experiment validated the ability of miR-103 to alleviate the cellular effect of circRNA-11228. Taken together, our findings indicate that the binding of circRNA-11228 to miR-103 inhibits the expression of the target gene INSIG, thereby regulating milk fat production in BMECs. This study offers novel insights into producing high-quality milk and new ways to improve the dietary composition of residents.

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Circ007071 Inhibits Unsaturated Fatty Acid Synthesis by Interacting with miR-103-5p to Enhance PPARγ Expression in the Dairy Goat Mammary Gland

Cited by 7 publications

References 42 publications

Progress of Conjugated Linoleic Acid on Milk Fat Metabolism in Ruminants and Humans

Progress of Conjugated Linoleic Acid on Milk Fat Metabolism in Ruminants and Humans

Understanding Circular RNAs in Health, Welfare, and Productive Traits of Cattle, Goats, and Sheep

The Molecular Mechanism of circRNA-11228/miR-103/INSIG1 Pathway Regulating Milk Fat Synthesis in Bovine Mammary Epithelial Cells

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