Circ-SFMBT2 promotes the proliferation of gastric cancer cells through sponging miR-182-5p to enhance CREB1 expression

Abstract

BackgroundCircular RNAs(circRNAs) have been reported as a diverse class of endogenous RNA that regulate gene expression in eukaryotes. Recent evidence suggested that many circular RNAs can act as oncogenes or tumor suppressors through sponging microRNAs. However, the function of circular RNAs in gastric cancer remains largely unknown.Materials and methodsThe circRNA levels in gastric carcinoma tissues and plasmas were detected by real-time quantitative reverse transcription-polymerase chain reaction. The correlation between the expression of circRNA and clinic pathological features was analyzed. Rate of inhibiting of proliferation was measured using a CCK-8 cell proliferation assay. Clone formation ability was assessed with a clone formation inhibition test. We used the bioinformatics software to predict circRNA-miRNA and miRNA-mRNA interactions. Relative gene expression was assessed using quantitative real-time polymerase chain reaction and relative protein expression levels were determined with western blotting. CircRNA and miRNA interaction was confirmed by dual-luciferase reporter assays.ResultsWe characterized that one circRNA named circ-SFMBT2 showed an increased expression level in gastric cancer tissues compared to adjacent non-cancerous tissues and was associated with higher tumor stages of gastric cancer. Silencing of circ-SFMBT2 inhibited the proliferation of gastric cancer cells significantly. Importantly, we demonstrated that circ-SFMBT2 could act as a sponge of miR-182-5p to regulate the expression of CREB1 mRNA, named as cAMP response element binding protein 1, and further promote the proliferation of gastric cancer cells.ConclusionOur study reveals that circ-SFMBT2 participates in progression of gastric cancer by competitively sharing miR-182-5p with CREB1, providing a novel target to improve the treatment of gastric cancer. mutation-analysis-of-beta-thalassemia-in-east-western-indian-populatio-peer-reviewed-article-TACG for an example.

RETRACTED ARTICLE: Circ_0008035 contributes to cell proliferation and inhibits apoptosis and ferroptosis in gastric cancer via miR-599/EIF4A1 axis

“…For example, circ_104916 played a suppressive role in GC development via inhibiting cell growth and metastasis [6]. Circ-SFMBT2 was increased in GC and the increase of circ-SFMBT2 facilitated GC cell progression [7]. The level of circ_0000190 was associated with lymphatic metastasis, distal metastasis and tumor diameter [8].…”

Identification and Comparison of Novel Circular RNAs With Associated Co-expression and Competing Endogenous Rna Networks in Postmenopausal Osteoporosis

“…CircNHSL1 was also reported to intervene gastric cancer progression by sponging miR-1306-3p, and could serve as a novel biomarker for early diagnosis of gastric cancer [21]. Similarly, highly expressed circ-SFMBT2 was observed in gastric cancer tissues, and proved to participate in the pathogenesis of gastric cancer via sponging miR-182-5p [22]. To date, only a few pathogenic circRNAs were reported in osteoporosis, and RNA sequencing tools and bioinformatics analysis were not employed in these studies [23][24][25].…”

Progress of research into circular RNAs in urinary neoplasms

“…This is the primary mechanism involved in the circRNA regulation of gene expression. circ-SFMBT2 can serve as a sponge for miR-182-5p to regulate CREB1 mRNA expression and promote the proliferation of gastric cancer cells (Sun et al, 2018). circ-0000523 inhibits colorectal cancer, and its overexpression attenuates the proliferation of cancer cells and induces apoptosis.…”