Ciprofloxacin-resistant<i>Campylobacter</i>persists in raw retail chicken after the fluoroquinolone ban

Nannapaneni, Ramakrishna; Hanning, Irene; Wiggins, Keith C.; Story, Robert; Ricke, Steven C.; Johnson, Michael G.

doi:10.1080/02652030903013294

Cited by 23 publications

(17 citation statements)

References 14 publications

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“…; Nannapaneni et al . ) emphasize the persistence of ciprofloxacin‐resistant Campylobacter even without antimicrobial selection pressure. Moreover, tetracycline resistance is commonly detected in Camp.…”

Section: Discussionmentioning

confidence: 99%

Absence of tetracycline resistance inCampylobacter coliisolates from Finnish finishing pigs treated with chlortetracycline

Juntunen

Laurila²,

Heinonen³

et al. 2013

J Appl Microbiol

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Aims: To determine whether therapeutic treatment of pigs with chlortetracycline affects the susceptibility of their Campylobacter isolates for tetracycline, ciprofloxacin and erythromycin. Methods and Results: Minimum inhibitory concentrations (MICs) and presence of a tetracycline resistance gene tet(O) were studied in Campylobacter collected before, during and after chlortetracycline treatment. Tetracycline MICs and the presence of tet(O) for additional Campylobacter coli isolates collected previously from seven farrowing farms were also determined. Isolates with ciprofloxacin MICs above the epidemiological cut-off value (ECOFF) were subtyped by flaA restriction fragment length polymorphism (RFLP). Tetracycline MICs of 221 Camp. coli isolates remained under the ECOFF at all sampling stages as well as the MICs for 63 isolates from the other farms. The ciprofloxacin MIC was above the ECOFF for 22% of the isolates, and one Camp. coli isolate had an erythromycin MIC above the ECOFF. None of the studied 300 Campylobacter isolates from nine herds carried tet(O). flaA-RFLP typing revealed the heterogeneity of Camp. coli isolates with high ciprofloxacin MICs. Conclusion: Use of chlortetracycline did not increase the MIC values for the antimicrobials studied. Significance and Impact of the Study: This study demonstrated that susceptibility of Camp. coli isolates is not affected by chlortetracycline therapy if tet(O) is not present in Camp. coli population.

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Section: Discussionmentioning

confidence: 99%

Absence of tetracycline resistance inCampylobacter coliisolates from Finnish finishing pigs treated with chlortetracycline

Juntunen

Laurila²,

Heinonen³

et al. 2013

J Appl Microbiol

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“…For example, a clinical decline in ciprofloxacin resistance was observed after the 2005 ban of fluoroquinolones in poultry. 31 Despite the paucity of definitive data, a better argument for restricting antibiotics in agriculture may be to reduce the prevalence of resistant pathogens and, thereby, lower the chances for horizontal transfer of resistance genes.…”

Section: Clindamycin-resistant Group B Streptococcusmentioning

confidence: 99%

Overview: Global and Local Impact of Antibiotic Resistance

Watkins

Bonomo

2016

Infectious Disease Clinics of North America

193

187

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“…2009), studies following the enrofloxacin ban in the United States have shown persistence of fluoroquinolone resistance in campylobacters recovered from poultry products, a few years after cessation of the ban (Price et al. 2007; Nannapaneni et al. 2009).…”

Section: Discussionmentioning

confidence: 99%

Selecting for development of fluoroquinolone resistance in a Campylobacter jejuni strain 81116 in chickens using various enrofloxacin treatment protocols

Stapleton¹,

Cawthraw²,

Cooles³

et al. 2010

Journal of Applied Microbiology

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Aims: To determine the effect of various enrofloxacin dose regimes on the colonization and selection of resistance in Campylobacter jejuni strain 81116P in experimentally colonized chickens. Methods and Results: Two experiments were undertaken, in which 14‐day‐old chickens were colonized with 1 × 107–1 × 109 CFU g−1Camp. jejuni strain 81116P and then treated with enrofloxacin at 12–500 ppm in drinking water for various times. Caecal colonization levels were determined at various time‐points after start‐of‐treatment, and the susceptibility of recovered isolates to ciprofloxacin was monitored. Resistance was indicated by growth on agar containing 4 μg ml−1 ciprofloxacin, MICs of 16 μg ml−1 and the Thr86Ile mutation in gyrA. Enrofloxacin at doses of 12–250 ppm reduced Camp. jejuni colonization over the first 48–72 h after start‐of‐treatment. The degree of reduction in colonization was dose, but not treatment time, dependent. In all cases, maximal colonization was re‐established within 4–6 days. Fluoroquinolone‐resistant organisms were recoverable within 48 h of start‐of‐treatment; after a further 24 h all recovered isolates were resistant. In contrast, a dose of 500 ppm enrofloxacin reduced colonization to undetectable levels within 48 h, and the treated birds remained Campylobacter negative throughout the remaining experimental period. By high pressure liquid chromatography, for all doses, the maximum concentrations of enrofloxacin and ciprofloxacin in the caecal contents were detected at the point of treatment completion. Thereafter, levels declined to undetectable by 7 days post‐treatment withdrawal. Conclusions: In a model using chickens maximally colonized with Camp. jejuni 81116P, treatment with enrofloxacin, at doses of 12–250 ppm in drinking water, enables the selection, and clonal expansion, of fluoroquinolone‐resistant organisms. However, this is preventable by treatment with 500 ppm of enrofloxacin. Significance and impact of the study: Treatment of chickens with enrofloxacin selects for resistance in Camp. jejuni in highly pre‐colonized birds. However, a dose of 500 ppm enrofloxacin prevented the selection of resistant campylobacters.

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Ciprofloxacin-resistantCampylobacterpersists in raw retail chicken after the fluoroquinolone ban

Cited by 23 publications

References 14 publications

Absence of tetracycline resistance inCampylobacter coliisolates from Finnish finishing pigs treated with chlortetracycline

Absence of tetracycline resistance inCampylobacter coliisolates from Finnish finishing pigs treated with chlortetracycline

Overview: Global and Local Impact of Antibiotic Resistance

Selecting for development of fluoroquinolone resistance in a Campylobacter jejuni strain 81116 in chickens using various enrofloxacin treatment protocols

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