Ciprofloxacin Resistance in Domestic Wastewater Treatment Plants

Manaia, Célia M.; Novo, Ana; Coelho, Bruno; Nunes, Olga C.

doi:10.1007/s11270-009-0171-0

Cited by 56 publications

(23 citation statements)

References 28 publications

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“…Inevitably, these bacteria harbour antibiotic resistance genes, frequently associated with mobile genetic elements, which means that these genes have a high potential to be propagated amongst the bacterial community Vaz-Moreira et al, 2014). In part this situation can be attributed to the fact that although conventional secondary waste-water treatment processes can reduce the cultivable bacterial loads around 2 logarithmic cycles, it fails to reduce the prevalence of antibiotic resistance and, sometimes, can even contribute for its increase (Ferreira da Silva et al, 2006;Luczkiewicz et al, 2010;Manaia et al, 2010;Novo et al, 2013;Rizzo et al, 2013;Zhang et al, 2009). This means that treated wastewater will contain high doses of antibiotic resistant bacteria.…”

Section: Risks and Precautions Associated With Antibiotic Resistancementioning

confidence: 99%

Wastewater reuse in irrigation: A microbiological perspective on implications in soil fertility and human and environmental health

Becerra-Castro

Lopes

Vaz‐Moreira

et al. 2015

Environment International

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396

211

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The reuse of treated wastewater, in particular for irrigation, is an increasingly common practice, encouraged by governments and official entities worldwide. Irrigation with wastewater may have implications at two different levels: alter the physicochemical and microbiological properties of the soil and/or introduce and contribute to the accumulation of chemical and biological contaminants in soil. The first may affect soil productivity and fertility; the second may pose serious risks to the human and environmental health. The sustainable wastewater reuse in agriculture should prevent both types of effects, requiring a holistic and integrated risk assessment. In this article we critically review possible effects of irrigation with treated wastewater, with special emphasis on soil microbiota. The maintenance of a rich and diversified autochthonous soil microbiota and the use of treated wastewater with minimal levels of potential soil contaminants are proposed as sine qua non conditions to achieve a sustainable wastewater reuse for irrigation.

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Section: Risks and Precautions Associated With Antibiotic Resistancementioning

confidence: 99%

Wastewater reuse in irrigation: A microbiological perspective on implications in soil fertility and human and environmental health

Becerra-Castro

Lopes

Vaz‐Moreira

et al. 2015

Environment International

Self Cite

396

211

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“…A domestic wastewater treatment plant ( WWTP ) discharges about 1 billion (10 9 ) ciprofloxacin resistant coliforms per minute. Total and ciprofloxacin resistant coliforms ( CFU per day) discharged by WWTP in different countries [ WWTP 1‐ WWTP 5, Portugal ( PT ); WWTP 6, Poland ( PL ); WWTP 7, Ireland ( IE )], with different sizes (average day flow of 20 000, 32 500, 900, 890, 200, 96 000 and 49 000 m 3 , respectively) and treatment processes [activated sludge ( WWTP 1 and WWTP 6), trickling filter ( WWTP 2), submerged aerated filter ( WWTP 3), aeration lagoon ( WWTP 4), anaerobic lagoon ( WWTP 5), unknown secondary treatment ( WWTP 7), with bacterial removal rates above of 1.5–4 log ( CFU ; Galvin et al ., ; Łuczkiewicz et al ., ; Manaia et al ., ; Novo & Manaia, ).…”

Section: Bacterial Diversity In Water Habitatsmentioning

confidence: 99%

“…A domestic wastewater treatment plant (WWTP) discharges about 1 billion (10 9 ) ciprofloxacin resistant coliforms per minute. Total and ciprofloxacin resistant coliforms (CFU per day) discharged by WWTP in different countries [WWTP1-WWTP5, Portugal (PT); WWTP6, Poland (PL); WWTP7, Ireland (IE)], with different sizes (average day flow of 20 000, 32 500, 900, 890, 200, 96 000 and 49 000 m 3 , respectively) and treatment processes [activated sludge (WWTP1 and WWTP6), trickling filter (WWTP2), submerged aerated filter (WWTP3), aeration lagoon (WWTP4), anaerobic lagoon (WWTP5), unknown secondary treatment (WWTP7), with bacterial removal rates above of 1.5-4 log (CFU; Galvin et al, 2010;Łuczkiewicz et al, 2010;Manaia et al, 2010;. negative impacts of this procedure have been demonstrated and include the persistence of antimicrobial residues in water and fish and the selection and spread of resistance genes, with the consequent contamination of the environment and the human foodchain (Sørum, 1998;Cabello, 2006;Taylor et al, 2011;Tamminen et al, 2011).…”

Section: Antibiotic Resistance In Aquaculture Environmentsmentioning

confidence: 99%

Bacterial diversity and antibiotic resistance in water habitats: searching the links with the human microbiome

2014

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Water is one of the most important bacterial habitats on Earth. As such, water represents also a major way of dissemination of bacteria between different environmental compartments. Human activities led to the creation of the so-called urban water cycle, comprising different sectors (waste, surface, drinking water), among which bacteria can hypothetically be exchanged. Therefore, bacteria can be mobilized between unclean water habitats (e.g. wastewater) and clean or pristine water environments (e.g. disinfected and spring drinking water) and eventually reach humans. In addition, bacteria can also transfer mobile genetic elements between different water types, other environments (e.g. soil) and humans. These processes may involve antibiotic resistant bacteria and antibiotic resistance genes. In this review, the hypothesis that some bacteria may share different water compartments and be also hosted by humans is discussed based on the comparison of the bacterial diversity in different types of water and with the human-associated microbiome. The role of such bacteria as potential disseminators of antibiotic resistance and the inference that currently only a small fraction of the clinically relevant antibiotic resistome may be known is discussed.

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“…The proportions of ciprofloxacin resistant faecal coliforms were 31.42% in WWTP A effluent and 26.47% in WWTP B. This resistance rate is higher in comparison to reported levels of ciprofloxacin resistance in the E. coli isolated from other WWTPs (59, 62, 63). In general, differences in antibiotic resistance percentages were observed between the two WWTPs, particularly for colistin, trimethoprim and kanamycin (Figure 1).…”

Section: Discussionmentioning

confidence: 64%

Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

Smyth

O’Flaherty

Walsh

et al. 2019

Preprint

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26Wastewater treatment plants (WWTPs) provide optimal conditions for the maintenance and 27 spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this 28 work we describe the occurrence of antibiotic resistant faecal coliforms and their mechanisms 29 of antibiotic resistance in the effluent of two urban WWTPs in Ireland. Effluent samples were 30 collected from two WWTPs in Spring and Autumn of 2015 and 2016. The bacterial 31 susceptibility patterns to 13 antibiotics were determined. The phenotypic tests were carried out 32 to identify AmpC or extended-spectrum β-lactamase (ESBL) producers. The presence of ESBL 33 genes were detected by PCR. Plasmids carrying ESBL genes were transformed into 34Escherichia coli DH5α recipient and underwent plasmid replicon typing to identify 35 incompatibility groups. More than 90% of isolated faecal coliforms were resistant to 36 amoxicillin and ampicillin, followed by tetracycline (up to 39.82%), ciprofloxacin (up to 37 31.42%) and trimethoprim (up to 37.61%). Faecal coliforms resistant to colistin and imipenem 38 were detected in all effluent samples. Up to 53.98% of isolated faecal coliforms expressed a 39 multi-drug resistance (MRD) phenotype. AmpC production was confirmed in 5.22% of 40 isolates. The ESBL genes were confirmed for 11.84% of isolates (9.2% of isolates carried 41 blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15). Plasmids extracted from 52 ESBL 42 isolates were successfully transformed into recipient E. coli. The detected plasmid 43 incompatibility groups included the IncF group, IncI1, IncHI1/2 and IncA/C. These results 44 provide evidence that treated wastewater is polluted with ARB and MDR faecal coliforms and 45 are sources of ESBL-producing, carbapenem and colistin resistant Enterobacteriaceae. 46 47 48 49 Keywords 50 Antibiotic resistance, multidrug resistance, AmpC, extended-spectrum β-lactamase (ESBL), 51 plasmids 52 53 54 55 56 3

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Ciprofloxacin Resistance in Domestic Wastewater Treatment Plants

Cited by 56 publications

References 28 publications

Wastewater reuse in irrigation: A microbiological perspective on implications in soil fertility and human and environmental health

Wastewater reuse in irrigation: A microbiological perspective on implications in soil fertility and human and environmental health

Bacterial diversity and antibiotic resistance in water habitats: searching the links with the human microbiome

Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

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