Ciprofloxacin-induced DNA damage in primary culture of rat astrocytes and protection by Vitamin E

Gürbay, Aylin; Gonthier, B.; Signorini-Allibe, N.; Barret, L.; Favier, A.; Hıncal, Filiz

doi:10.1016/j.neuro.2005.05.007

Cited by 58 publications

(36 citation statements)

References 27 publications

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“…Moreover, vitamin E has been shown to protect cartilage tissues from ciprofloxacininduced lesions [23]. In astrocytes cultures, ciprofloxacininduced DNA-damage was restored by vitamin E application suggesting that DNA-damage is mediated by oxidative stress [12]. Furthermore, a-tocopherol acetate supplementation reduced enrofloxacin residues in turkey meat, which indicate a possible interaction between both molecules that reduces the amount of enrofloxacin in tissues [4,25].…”

Section: Discussionmentioning

confidence: 98%

“…Furthermore, a-tocopherol acetate supplementation reduced enrofloxacin residues in turkey meat, which indicate a possible interaction between both molecules that reduces the amount of enrofloxacin in tissues [4,25]. Studies on phototoxic actions of fluoroquinolones revealed that these toxic effects are related to induction of reactive oxygen species [12,23,[27][28][29][30].…”

Section: Discussionmentioning

confidence: 98%

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Ciprofloxacin-Induced Antibacterial Activity is Reversed by Vitamin E and Vitamin C

et al. 2012

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In the present study, we investigated the possible involvement of oxidative stress in ciprofloxacin-induced cytotoxicity against several reference bacteria including Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, and clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA). Oxidative stress was assessed by measurement of hydrogen peroxide generation using a FACScan flow cytometer. The antibacterial activity of ciprofloxacin was assessed using the disk diffusion method and by measuring the minimum inhibitory concentration (MIC). Ciprofloxacin induced a dose-dependent antibacterial activity against all bacteria where the highest tested concentration was 100 ug/ml. Results revealed that E. coli cells were highly sensitive to ciprofloxacin (MIC = 0.21 μg/mL ± 0.087), P. aeruginosa and S. aureus cells were intermediately sensitive (MIC = 5.40 μg/mL ± 0.14; MIC = 3.42 μg/mL ± 0.377, respectively), and MRSA cells were highly resistant (MIC = 16.76 μg/mL ± 2.1). Pretreatment of E. coli cells with either vitamin E or vitamin C has significantly protected cells against ciprofloxacin-induced cytotoxicity. These results indicate the possible antagonistic properties for vitamins C or E when they are used concurrently with ciprofloxacin.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Ciprofloxacin-Induced Antibacterial Activity is Reversed by Vitamin E and Vitamin C

et al. 2012

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“…The drug generally binds to the A subunit of the bacterial enzyme DNA gyrase and blocks DNA replication, which leads to rapid killing of bacteria through additional processes. Several side effects of Cipro as observed in humans include DNA damage [3], severe liver damage [4], and hematuria [5]. Also, Cipro is much less absorbed by the gastrointestinal tract in bioactive form than other fluoroquinolones [6].…”

Section: Introductionmentioning

confidence: 99%

Detection of Ciprofloxacin in Urine through Sensitized Lanthanide Luminescence

Singha

Ahn

2016

Sensors

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Ciprofloxacin, a fluoroquinolone antibiotic, is widely used for the treatment of bacterial infection in humans due to its broad antibacterial spectrum. An excessive use or overdose of ciprofloxacin on the other hand can cause several adverse effects not only to humans but also to microorganisms. Unabsorbed ciprofloxacin in the body is mostly excreted through urine and finally goes to the environment, providing a drug resistance pressure on bacteria. Hence a simple and efficient detection method of ciprofloxacin is necessary, which, for example, can be used to analyze ciprofloxacin content in urine. Although ciprofloxacin itself shows inherent fluorescence, direct fluorescent detection of ciprofloxacin in raw urine sample is difficult due to autofluorescence of urine by other components. Herein we report that a Tb(III) complex of DO3A (1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid) can be efficiently sensitized by ciprofloxacin to emit luminescence separately from the urine autofluorescence wavelength region. Tb-DO3A shows excellent sensitivity with a detection limit of three parts per billion in aqueous buffer solution. Further, Tb-DO3A is used to detect ciprofloxacin with high sensitivity and selectivity in a raw urine sample without any purification or separation procedures in the concentrations ranging from 1 µg·mL−1 to 50 µg·mL−1. The direct measurement of ciprofloxacin excreted in urine may be used to control overdose of the drug.

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“…In rats given very high doses of h-tocopheryl acetate by gavages ≥125 mg/kg BW/day, TSH levels were elevated by 30-100%. At a very high dose of about 500 mg/kg BW/day biochemical indices of hepatotoxicity (serum alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were elevated (Gü rbay et al 2006). Trained rats had a significantly lower concentration of insulin in blood plasma.…”

Section: Discussionmentioning

confidence: 96%

The influence of α-tocopherol supplementation on plasma concentration of this vitamin and insulin in sedentary or physically trained rats

Debski

Gralak²,

Gronowska-Senger³

et al. 2011

Polish Journal of Veterinary Sciences

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Male young growing rats of Wistar strain (n=54) were fed a vitamin E deficient diet for 3 days of adaptation period and then 10 days of experimental period. After adaptation half of the rats ran, once per a day, on a treadmill with a rate of 2.0 m/s for 15 minutes for the following 10 days. Animals were given orally 0, 0.5, 1.0 or 4.0 mg/d/rat of α-tocopherol. The aim of the study was to evaluate the influence of physical training on tocopherol and insulin concentrations in the blood plasma of rats treated with different doses of vitamin E. The concentrations of α-tocopherol (HPLC-UV method used) and insulin (rat insulin specific RIA method used) were estimated in the blood plasma. Lower concentration of vitamin E in physically trained animals was observed, which may suggest increased needs of organisms for antioxidants, as a result of increased free radical production. In trained rats a lower concentration of plasma insulin was also observed. This effect is probably related to improvement in insulin action observed in organisms under physical exercise. The most significant impact on plasma α-tocopherol was due to its supplementation level. The treatment of rats with different doses of α-tocopherol was found to be not related to insulin concentration in plasma. A significant increase in feed conversion factor was noted in vitamin E treated animals. The obtained results suggest that the increased requirement of trained rats for antioxidants might be covered by α-tocopherol supplementation.

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Ciprofloxacin-induced DNA damage in primary culture of rat astrocytes and protection by Vitamin E

Cited by 58 publications

References 27 publications

Ciprofloxacin-Induced Antibacterial Activity is Reversed by Vitamin E and Vitamin C

Ciprofloxacin-Induced Antibacterial Activity is Reversed by Vitamin E and Vitamin C

Detection of Ciprofloxacin in Urine through Sensitized Lanthanide Luminescence

The influence of α-tocopherol supplementation on plasma concentration of this vitamin and insulin in sedentary or physically trained rats

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