Cinematographic analysis of bovine embryo development in serum-free oviduct-conditioned medium

Grisart, Bernard; Massip, A.; Dessy, F.

doi:10.1530/jrf.0.1010257

Cited by 124 publications

(82 citation statements)

References 1 publication

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“…The aim of these experiments was to determine when exactly FCS exerts its accelerating effect. The kinetics of development in unsupplemented mSOF recorded in Expts 2 and 3 was similar to that previously recorded in serum-free bovine oviduct conditioned medium (BOEC CM) (Grisart et al, 1994) (Gordon, 1994;Hasler et al, 1995;Massip et al, 1995 (Hackett et al, 1993), and the kinetics may differ if embryos originate from superovulated (Barnes and Eyestone, 1990) or from unstimulated cows (Hamilton and Laing, 1946;Thibault, 1966) as pointed out by Betteridge and Fléchon (1988).…”

Section: Time-lapse Cinematographic Equipmentsupporting

confidence: 84%

“…The cinematographic chamber, gas and thermoregulating systems were as described by Grisart et al (1994) (Dagnelie, 1986 (Bavister et al, 1992;Lim et al, 1994;Pinyopummintr and Bavister, 1994;Rorie et al, 1994;Carolan et al, 1995 (Walker et al, 1992;Bavister, 1995;Carolan et al, 1995). This faster development might explain why blastocysts at day 8 produced in mSOF containing serum contained more cells than did those obtained under serum-free culture conditions.…”

Section: Time-lapse Cinematographic Equipmentmentioning

confidence: 99%

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Effects of supplementation with fetal calf serum on development of bovine embryos in synthetic oviduct fluid medium

Langendonckt¹,

Donnay²,

Schuurbiers³

et al. 1997

Reproduction

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103

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Section: Time-lapse Cinematographic Equipmentsupporting

confidence: 84%

Section: Time-lapse Cinematographic Equipmentmentioning

confidence: 99%

Effects of supplementation with fetal calf serum on development of bovine embryos in synthetic oviduct fluid medium

Langendonckt¹,

Donnay²,

Schuurbiers³

et al. 1997

Reproduction

Self Cite

103

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show abstract

“…Time-lapse monitoring has been used for decades to study embryonic development in animals [8,9,[19][20][21]. The introduction of incubators designed for clinical use enables continuous monitoring of human embryos in assisted reproduction, which allows for evaluation of all the morphological parameters already implemented in clinical practice at the corresponding points of time.…”

Section: Discussionmentioning

confidence: 99%

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Kirkegaard

Hindkjær

Grøndahl

et al. 2012

J Assist Reprod Genet

128

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Purpose Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing timelapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator. Methods In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer. Results No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR TLI/COI : 0.81 (0.65; 1.02)) or PP (RR TLI/COI : 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR TLI/COI : 0.96 (0.73; 1.26)); PP (RR TLI/COI : 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR TLI/COI : 1.09 (0.84; 1.41)); PP (RR TLI/COI : 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate. Conclusion Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.

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“…Although previous studies in bovine have linked oocyte diameter and early developmental kinetics with blastocyst formation and viability (Van Soom et al 1992, Grisart et al 1994, Fair et al 1995, Otoi et al 1997, their relation with the incidence of apoptosis during embryo development remains unexplored. Suboptimal culture conditions undoubtedly contribute to embryonic apoptosis, but the intrinsic quality of the oocyte and the embryo may also be a causative factor.…”

Section: Introductionmentioning

confidence: 99%

Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity

Vandaele

Mateusen²,

Maes³

et al. 2007

Reproduction

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Embryo quality is most frequently evaluated at the blastocyst stage, although quality parameters further back along the developmental axis, such as early developmental kinetics or oocyte quality, can be equally valuable. Despite the fact that previous studies in bovine have linked oocyte diameter and early developmental kinetics with blastocyst formation and viability, their relation with the incidence of apoptosis during embryo development remains relatively unexplored. Therefore, we related noninvasive parameters of oocyte and embryo quality, such as embryo kinetics, embryo morphology, and oocyte diameter, to the incidence of apoptosis throughout embryo development using fluorescent detection of active caspase-3 and -7. First, bovine in vitro embryos were selected according to developmental kinetics and morphology at four set times during culture and subjected to fluorescent detection of active caspase-3 and -7. Caspase activity was significantly higher in slow developing embryos in comparison with fast cleavers (P!0.05), but was not related to embryo morphology. Second, bovine oocytes were divided into three groups on the basis of oocyte diameter and the resulting embryos were used for staining at the same four set times. Caspase activity was significantly higher in embryos derived from growing oocytes compared with those of fully grown oocytes at 45, 80, and 117 hours post-insemination (hpi; P!0.05), but not at 168 hpi. Reproduction (2007) 133 709-718

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Cinematographic analysis of bovine embryo development in serum-free oviduct-conditioned medium

Cited by 124 publications

References 1 publication

Effects of supplementation with fetal calf serum on development of bovine embryos in synthetic oviduct fluid medium

Effects of supplementation with fetal calf serum on development of bovine embryos in synthetic oviduct fluid medium

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity

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