Effects of supplementation with fetal calf serum on development of bovine embryos in synthetic oviduct fluid medium

Langendonckt, Anne Van; Donnay, Isabelle; Schuurbiers, N; Auquier, Pascal; Carolan, C.; Massip, A.; Dessy, F.

doi:10.1530/jrf.0.1090087

Cited by 103 publications

(65 citation statements)

References 9 publications

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“…Co-culture systems for mammalian embryos normally contain serum, as much for the benefit of the coculture cells as for possible benefits to the embryos. Addition of serum to the culture medium increases the speed of development manifested in a lack of normal morula compaction and an earlier appearance of the blastocyst [8,23]. This fact is well stated in our results which showed higher Day 6 blastocysts rates in embryos developed with FCS (Groups Vero and FCS), than in the group SOF-BSA.…”

Section: Discussionsupporting

confidence: 86%

“…The percentage of early embryos that developed to blastocysts is similar for a variety of cells used in co-culture [17,22,23]. Also, it has been shown that when either B2 [38] or TCM199 [17,22,23] were combined with somatic cells in a co-culture system, both media supported a higher rate of development to blastocysts than did control media without cells. Hasler [39], showed that embryos cultured in a B2-BRL coculture exhibited faster development, with a higher total number of blastocysts and more cells than B2 medium without co-culture.…”

Section: Discussionmentioning

confidence: 87%

“…Although the role of serum is not completely known (for review, see [21]) it has been demonstrated that serum has a biphasic effect inhibiting early cleavage divisions and accelerating the development of morula and blastocysts [22][23][24]. It is also expected that serum provides energy substrates, amino-acids, vitamins, growth factors and heavy-metal chelators at concentrations that vary among batches [25].…”

Section: Introductionmentioning

confidence: 99%

“…It is also expected that serum provides energy substrates, amino-acids, vitamins, growth factors and heavy-metal chelators at concentrations that vary among batches [25]. A higher development rate to the blastocyst stage is obtained from media supported with serum, but the serum effectiveness for the vitro embryo production might change considerably from one batch to another [23]. Furthermore, embryos produced with serum show alterations in their ultrastructure, impaired compaction, abnormal blastulation, aberrant mRNA expression profiles and large calf syndrome with greater incidences of stillbirths and deaths after birth [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

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Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

et al. 2008

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The aim of this study was to examine the effects of co-culture with Vero cells during the in vitro maturation (IVM) and three culture media, B2 + 5% fetal calf serum (FCS) on Vero cells, synthetic oviduct fluid (SOF) + 5% FCS, and SOF + 20 g L À1 bovine serum albumin (BSA), on the developmental competence of the embryos and their ability to survive vitrification/warming. We also tested the effect of morphological quality and the age of the embryo on its sensitivity to vitrification. The IVM system neither affects the embryo development up to Day 7 nor survival rates after vitrification. The culture of embryos in SOF + FCS and in Vero cells + B2 allowed obtaining more Day 6 and Day 7 blastocysts, and a higher % of Day 7 blastocysts vitrified than culture in SOF + BSA. Contrarily, on Day 8, more blastocysts were vitrified in SOF + BSA than in SOF + FCS. Blastocysts quality affected development after vitrification/warming, and Day 7 embryos showed higher survival rates than their Day 8 counterparts. Day 7 blastocysts produced in Vero cells or in SOF + BSA survived at higher rates than those produced in SOF + FCS at 24 and 48 h after warming. Embryo culture with BSA allows obtaining hatching rates after vitrification/warming higher than those obtained after coculture with Vero cells in B2 and FCS. Moreover, this system provides hatching rates from Day 8 blastocysts comparable to those obtained on Day 7 in Vero cells. Further studies, including embryo transfer to recipients, are needed to clarify factors affecting the freezability of in vitro produced bovine embryos. #

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

et al. 2008

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“…Although culture conditions can in¯uence the kinetics of early development (Pinyopummintr and Bavister, 1994;Van Langendonckt et al, 1997), it is likely that the main factors controlling this parameter are intrinsic to the oocyte , the sperm (Eid et al, 1994;Comizzoli et al, 2000;present study) or both. In fact, in mice, a gene controlling the rate of preimplantation cleavage division and subsequent embryo survival (Ped: preimplantation embryo development) has been identi®ed (for review see, Warner et al, 1998).…”

Section: Discussionmentioning

confidence: 97%

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Ward

Rizos

Corridan

et al. 2001

Molecular Reproduction Devel

127

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The objectives of this study were: (1) to evaluate the effect of sire on the time from insemination to first cleavage following insemination in vitro and the relationship of this parameter to field fertility and (2) to establish the relationship between the kinetics of cleavage in vitro and oocyte developmental competence for bulls of known field fertility. Frozen semen from six bulls with 150-day non-return rates ranging from 57±78% was used. In experiment 1, after insemination with semen from one of the six bulls, presumptive zygotes were transferred to IVC in droplets of synthetic oviduct fluid. Droplets were examined at 24, 27, 30, 33, 36, 42, and 48 hr after insemination and the number of cleaved oocytes was recorded. Blastocyst yield was recorded on Days 6-, 7-, and 8-post insemination. In experiment 2, culture droplets were examined at 30, 36, and 48 hr after insemination. At each time point, the number of cleaved embryos was recorded and these embryos were transferred into new droplets and were cultured separately for the duration of the experiment. The proportion of embryos developing to the blastocyst stage was recorded for each of the groups for each bull. The best predictor of field fertility was a model containing 33-hpi-cleavage percentage only (r 0.689, P`0.0001). There was also a significant correlation between blastocyst yield and non-return rate, with Day 7 blastocyst yield having the highest correlation (r 0.356), although this was relatively low in comparison. In experiment 2, irrespective of sire, a significantly higher proportion of those early-cleaving oocytes (before 30 hpi) developed to blastocysts than those cleaving later. In most cases, a higher proportion of blastocysts derived from early-cleaving oocytes hatched from the zona pellucida suggesting that such blastocysts are of superior quality to those derived from late-cleaving oocytes. In conclusion these data confirm our earlier observations that earliest cleaving zygotes are more competent in terms of development to the blastocyst stage than those that cleave later. This phenomenon is independent of the sire used. However, we have demonstrated that the kinetics of early embryonic development as measured by the timing of the first cleavage division post insemination vary between different bulls and that these differences can be used to discriminate between bulls of high and low bull field fertility. Mol. Reprod. Dev. 60: 47±55,

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Glucose metabolism during bovine preimplantation development: Analysis of gene expression in single oocytes and embryos

et al. 1997

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Glucose metabolism of the bovine embryo is low during the first cleavages and increases sharply after the major resumption of the genome (8–16 cells). The mRNA level for genes involved in glucose metabolism was tested by RT‐PCR on individual oocytes and embryos at different stages of development. These genes were: glucose transport GLUT‐1, hexokinase (HK), glucose‐6‐phosphate‐dehydrogenase (G6PDH), and glucose‐phosphate‐isomerase (GPI); actin was used as a reference transcript. RT‐PCR results revealed three types of oocytes or embryos: positive with a PCR signal for each transcript considered, nul with no signal for any transcript, and heterogeneous with a PCR signal for some transcripts and none for others. The number of nul and heterogeneous samples was higher for slow than for fast‐cleaving embryos (81% vs. 36%), and the proportion of positive embryos increased significantly at the 16‐cell and morula stages (P < 0.002), suggesting a correlation between mRNA content and developmental capacity. In positive embryos, GLUT‐1 mRNA level was reduced by half during maturation and fertilization. Actin and hexokinase mRNA levels decreased during the first cleavages, but significantly increased at the 16‐cell and morula stages, respectively. GPI transcript remained stable throughout development, whereas there was a significant rise for G6PDH at the 4‐cell stage, perhaps due to a polyadenylation process. Finally, the absence or decrease in intensity of several transcripts at the blastocyst stage suggests suboptimal culture conditions. Mol. Reprod. Dev. 48:216–226, 1997. © 1997 Wiley‐Liss, Inc.

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Effects of supplementation with fetal calf serum on development of bovine embryos in synthetic oviduct fluid medium

Abstract: The effects of fetal calf serum (FCS)

Cited by 103 publications

References 9 publications

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo

Glucose metabolism during bovine preimplantation development: Analysis of gene expression in single oocytes and embryos

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