Çin Tibet Koyununda Myogenin Geninin Genetik Polimorfizmi ve Büyüme Özellikleriyle Olan İlişkisi

Abstract

Myogenin gene encodes for skeletal muscle-specific transcription factors with highly conserved basic helix-loop-helix domain and play key role in growth and muscle development. In this study, the polymorphisms of myogenin gene were investigated to check whether they are associated with growth traits in Chinese-Tibetan sheep. In three sheep breeds with a total number of 632 individuals, three SNPs, including two in exon 1 (C109A and C183T) and one in intron 2 (A1403C) were found by DNA sequencing. Among those, C109A is a missense mutation (Gly37Arg). The single marker association analysis showed that the three mutations were significantly associated with growth traits (at P<0.01 or P<0.05). In conclusion, our results suggest that myogenin gene variation may be considered molecular markers for growth traits in Chinese-Tibetansheep. Keywords: Chinese Tibetan sheep, Myogenin gene, SNP, Growth traits Çin Tibet Koyununda Myogenin Geninin Genetik Polimorfizmi ve Büyüme Özellikleriyle Olan İlişkisi ÖzetMyogenin geni oldukça korunaklı olan temel heliks-ilmek-heliks domain yapısında olup iskelet kas-spesifik transkripsiyon faktörlerini kodlar ve büyüme ile kas gelişimde önemli rol oynar. Bu çalışmada, myogenin geninin polimorfizmi ve Çin-Tibet koyununda büyüme özellikleri ile ilgisinin olup olmadığı araştırıldı. Üç koyun ırkından oluşan toplam 632 hayvanda, DNA sekansı ile ikisi ekzon 1 (C109A ve C183T) ve biri intron 2 (A1403C)'de olmak üzere üç SNP bulundu. Bunlar arasında C109A missen mutasyonuydu (Gly37Arg). Tek markır asosiasyon analizi, üç mutasyonun anlamlı derecede büyüme özellikleri ile ilişkili olduğunu gösterdi (P<0.01 veya P<0.05). Sonuç olarak, elde edilen sonuçlar ışığında myogenin gen varyasyonunun Çin-Tibet koyununda büyüme özellikleri için moleküler belirteç olabileceği saptandı.

Polymorphisms in coding and non-coding regions of rabbit (Oryctolagus cuniculus) myogenin (MyoG) gene

“…Analyses of this gene were also performed within the Bovidea family, but with contradictory results. Some authors reported association with some important economically relevant body measurement (Bhuiyan et al, 2009, Xue et al, 2011, Sun and Han, 2017, while others reported lack of association between SNPs and growth traits (Abu El-Magd et al, 2015).…”

“…Reports have shown that SNPs within this gene in swine (Kapelański et al, 2005;Kim et al, 2009), cattle (Xue et al, 2011), sheep (Sun and Han, 2017) and chickens (Wei et al, 2016) influence growth traits. However most of the SNPs analysed were synonymous, intronic or 3' region.…”

“…However most of the SNPs analysed were synonymous, intronic or 3' region. Only Sun and Han (2017), in sheep, reported Gly37Arg substitution which is located outside bHLH. Association with meat weight in hind part and meat and bone weight in fore part of carcass SNP identified in 5' upstream sequence was found only in the NZW x BGG population, while for SNP within exon the hind part weight differed significantly only in the TER population, but it appears to be associated with dissectible bone weight, not dissectible meat weight (Table 3).…”

Tibet Koyunlarında Büyüme Hormonunu Salgılatıcı Hormon (GHRH) Genindeki Polimorfizmlerin Büyüme Özellikleri İle İlişkisi

“…Our previous work revealed that SNPs of candidate (i.e., GH and Myogenin) significantly influenced growth traits in Tibetan sheep [7,15] . Multi-marker interaction analysis suggested the influence of the mutations in three different genes were additive effects, which is not consistent with the the function of GHRH in regulating the GH secretion.…”

“…were designed by Primer Premier Software (Version 5.0). PCR amplification was performed following the method of Sun et al [7] Amplification were then sequenced using an ABI 3730 sequencer (ABI, Foster City, CA, USA).…”