Chrysin Sensitizes Human Lung Cancer Cells to Tumour Necrosis Factor Related Apoptosis-Inducing Ligand (TRAIL) Mediated Apoptosis

Mehdi, Syed Hassan; Zafaryab,; Nafees, Sana; Khan, Asad; Ahmad, Irfan; Hafeez, Zubair Bin; Rizvi, Moshahid Alam

doi:10.31557/apjcb.2019.4.2.27-33

Cited by 13 publications

(8 citation statements)

References 21 publications

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“…This reduction in cell viability induced by IL-2, TRAIL and MIX has also been demonstrated in MB49 tumor cells [20]. In the neutral red dye assay, a study showed that TRAIL decreased cell viability in lung cancer model (L-132) [49].…”

Section: Trail Induces Cell Death By Apoptosis When It Binds To Its R...mentioning

confidence: 56%

Anti-tumor effect of IL-2 and TRAIL expressed by recombinant Salmonella: an immunotherapeutic proposal against bladder cancer by iNOS, Caspases activation and modulation of the immune system

Fragelli

Rodolpho

Godoy

et al. 2022

Preprint

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IL-2 and TRAIL are therapeutic agents known for their antitumor role, but they can be cleared quickly by the body or be cytotoxic, compromising their function. To reverse this impasse, the synthesis of these agents by live bacteria directly in the tumor is a viable approach, due to bacteria’s preference to colonize tumor tissue. It happens because of the high nutrient grid in this microenvironment and possible escape from the immune system since solid tumors are ischemic and have regions of hypoxia. In this context, attenuated Salmonella represents a promising live vector for delivery of antitumor molecules. The present study investigated the effects of IL-2, TRAIL and mixture of proteins expressed by recombinant Salmonella, strain SL3261, with unique and innovative construction with IL-2 and TRAIL proteins, on bladder cancer cells and in mice od C57BL/6. Human bladder cancer cell line RT4 was exposed for 24 and 48 hours to these proteins and were analyzed with flow cytometry, ELISA, dyes and fluorescent antibodies. In the animal model, tumor induction was performed with MB49 cells, followed by treatment with bacterial strains and collection of tissues and organs for analysis. The results demonstrated that both agents are cytotoxic to the tumor cells, as they cause a decrease in cell viability, modification of its proper morphology and induction of apoptosis. This effect is caused by the activation of the iNOS enzyme by IL-2, which leads to the production of nitric oxide with consequent activation of genes that lead to DNA degradation and by the activation of the Caspase family by TRAIL, causing apoptosis. In mice, there was an important regression of tumor progression and modulation of the immune system. Therefore, IL-2, TRAIL and MIX synthesized by recombinant Salmonella show promising potential in bladder cancer therapy.

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Section: Trail Induces Cell Death By Apoptosis When It Binds To Its R...mentioning

confidence: 56%

Anti-tumor effect of IL-2 and TRAIL expressed by recombinant Salmonella: an immunotherapeutic proposal against bladder cancer by iNOS, Caspases activation and modulation of the immune system

Fragelli

Rodolpho

Godoy

et al. 2022

Preprint

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show abstract

“…The synergistic effect of TRAIL when combined with an anticancer agent is a promising strategy to overcome the resistance of cancerous cells toward TRAIL. For instance, the combinatorial treatment of chrysin and TRAIL considerably augments apoptosis in lung cancer cells (Mehdi et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Combination Therapy of TRAIL and Thymoquinone Induce Breast Cancer Cell Cytotoxicity-Mediated Apoptosis and Cell Cycle Arrest

Salam¹,

Abdrabou

Sharada

et al. 2021

Asian Pac J Cancer Prev

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Objective: Cancer is one of the leading causes of mortality in both developed and developing nations. The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is characterized by its ability to selectively trigger apoptosis in cancer cells. TRAIL-based interventions have led to the development of recombinant human (rhTRAIL) as a promising therapy for different types of human cancer. Thymoquinone (TQ) has been shown to exert anticancer effect. The aim of the current study is to investigate the anticancer effect of the combinatorial therapy of TRAIL+TQ against human breast cancer cells. Methods: To achieve this hypothesis, cytotoxicity using MTT assay, as well as apoptosis and cell cycle using flow cytometric technique were preceded against breast cancer MCF-7 and MDA-MB-231 cancerous cell lines. Results: The current study showed that TRAIL induced cell cycle arrest and apoptosis. Moreover, it inhibited proliferation of MDA-MB-231 cells more than MCF-7 cells. Adding TQ to TRAIL increased the chemo-sensitivity of MDA-MB-231, while overcame the MCF-7 resistance to TRAIL. Conclusion: In conclusion, there is a synergistic effect between TRAIL and TQ playing a therapeutic role in killing resistant breast cancer cells.

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“…This was allocated to enhanced expression of caspase‐3, 8, 9, Bax and alleviated expression of Bcl‐2 (Figure 5). Speaking concisely, Chrysin escalates TRAIL induced apoptosis by modulating caspases and cell survival protein Bcl‐2 (Mehdi et al, 2019). Chrysin in combination with cyclophosphamide provided chemoprotection and mitigated toxic effects (Lakshmi et al, 2019).…”

Section: Chrysin As Magic Bullet In Anticancer Therapymentioning

confidence: 99%

Plant flavone Chrysin as an emerging histone deacetylase inhibitor for prosperous epigenetic‐based anticancer therapy

Ganai

Sheikh

Baba

2020

Phytotherapy Research

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Aberrations in epigenetic mechanisms provide a fertile platform for tumour initiation and progression. Thus, agents capable of modulating the epigenetic environment of neoplasms will be a valuable addition to the anticancer therapeutics. Flavones are emerging as befitting anticancer agents due to their inherent antioxidant activity and the ability to restrain epi‐targets namely histone deacetylases (HDACs). HDACs have broader implications in pathogenesis of various cancers. Chrysin, a flavone possessing the ability to inhibit HDACs could prove as a potential anticancer drug. Thus, in this article we focussed on Chrysin and its distinct antineoplastic effect against bellicose malignancies including lung, colorectal, cervical, gastric, melanoma, hepatocellular carcinoma and breast cancer. The underlying signalling cascades triggered by Chrysin for inducing cytotoxic effect in these cancer models are discussed. Importantly, approaches towards combinatorial treatments by Chrysin and commercial anticancer agents are taken into account. The downstream molecular mechanism aroused by combined therapy for abrogating onerous cancer chemoresistance is delineated as well. Moreover, the nano‐combinatorial approach involving co‐encapsulation of Chrysin with other herbal and non‐herbal agents for clinical excellence is elucidated.

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Chrysin Sensitizes Human Lung Cancer Cells to Tumour Necrosis Factor Related Apoptosis-Inducing Ligand (TRAIL) Mediated Apoptosis

Cited by 13 publications

References 21 publications

Anti-tumor effect of IL-2 and TRAIL expressed by recombinant Salmonella: an immunotherapeutic proposal against bladder cancer by iNOS, Caspases activation and modulation of the immune system

Anti-tumor effect of IL-2 and TRAIL expressed by recombinant Salmonella: an immunotherapeutic proposal against bladder cancer by iNOS, Caspases activation and modulation of the immune system

Combination Therapy of TRAIL and Thymoquinone Induce Breast Cancer Cell Cytotoxicity-Mediated Apoptosis and Cell Cycle Arrest

Plant flavone Chrysin as an emerging histone deacetylase inhibitor for prosperous epigenetic‐based anticancer therapy

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