Chryseomicrobium imtechense gen. nov., sp. nov., a new member of the family Planococcaceae

Abstract

A Gram-stain-positive, rod-shaped, yellow, non-motile, non-spore-forming, strictly aerobic bacterial strain, designated MW 10 T , was isolated from seawater of the Bay of Bengal, India, and was subjected to a polyphasic taxonomic study. Analysis of the 16S rRNA gene sequence revealed that strain MW 10 T showed highest similarity to the type strains of Psychrobacillus psychrodurans (96.15 %) and Psychrobacillus psychrotolerans (96.01 %) and showed less than 96 % similarity to members of the genera Paenisporosarcina, Planococcus, Sporosarcina and Planomicrobium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain MW 10 T formed a clade separate from members of closely related genera. The morphological, physiological and chemotaxonomic characteristics of strain MW 10 T differed from those of members of closely related genera. The major fatty acid in strain MW 10 T was iso-C 15 : 0 and the menaquinones were MK-7 (48.4 %), MK-8 (32.3 %), MK-7(H 2 ) (13.7 %) and MK-6 (5.6 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unknown phospholipid, an unknown lipid and an unknown glycolipid. The cell-wall peptidoglycan type was L-Lys-D-Asp. The genomic DNA G+C content (53.4 mol%) of strain MW 10 T was significantly different from those of members of closely related genera. On the basis of its morphological, physiological and chemotaxonomic characteristics as well as our phylogenetic analysis, we conclude that strain MW 10 T is a member of a novel genus and species, for which the name Chryseomicrobium imtechense gen. nov., sp. nov., is proposed. The type strain of Chryseomicrobium imtechense is

Chryseomicrobium palamuruense sp. nov., a haloalkalitolerant bacterium isolated from a sediment sample

“…confirms data already published (Arora et al, 2011;Raj et al, 2013;Deng et al, 2014), and differs from strain PU1 T which contains L-Orn (Fig. S4).…”

“…Phylogenetic examinations strongly suggest that the recently described genus Chryseomicrobium (Arora et al, 2011) comprises the fifteenth genus of this family. Members of the genus Chryseomicrobium are aerobic, Gram-positive, non-endosporeforming rods and, at the time of writing, are represented by only three species with validly published names, Chryseomicrobium imtechense, Chryseomicrobium amylolyticum, and Chryseomicrobium aureum (Arora et al, 2011;Raj et al, 2013 ;Deng et al, 2014). In this paper, we describe a Gramstain-positive, rod-shaped, motile, non-endospore-forming bacterium (strain PU1 T ) that shows 99 % 16S rRNA gene sequence similarity with the type strains of other species of the genus Chryseomicrobium.…”

Biotransformation of 4-chloro-2-nitrophenol into 5-chloro-2-methylbenzoxazole by a marine Bacillus sp. strain MW-1

“…Identification of a 4C2NP decolourizing strain Marine strain MW-1 was identified on the basis of 16S rRNA gene sequencing using the universal primers 27f and 1492r (Arora et al 2011). …”

Chryseomicrobium aureum sp. nov., a bacterium isolated from activated sludge

“…At the time of writing, the genus comprises two species with validly published names: Chryseomicrobium imtechense (Arora et al, 2011) and Chryseomicrobium amylolyticum (Raj et al, 2013). In this study, we report on a Chryseomicrobiumlike bacterial strain, designated BUT-2 T , which was isolated from activated sludge.…”