Chryseobacterium mulctrae sp. nov., isolated from raw cow’s milk

Yoon, Sung-Hee; Lee, Jueun; Han, Rongkui; Kwon, Mirae; Kim, Geun‐Bae

doi:10.1099/ijsem.0.003647

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…Chryseobacterium species (Weeksellaceae) were significantly more abundant in the tank water samples where the control diet (A) was administered. These chemoorganotrophic bacteria can be recovered from different environments (soil, freshwater, drinking water, lactic acid beverages, marine sediment and permafrost) and are associated with a multitude of animals (midgut of mosquitoes, cockroach guts, millipede feces, penguin guano, gut homogenates of freshwater copepods, bird feathers, cow's milk, raw meats, and chicken) [43][44][45][46]. Chryseobacterium spp.…”

Section: A Focus On Water Ecosystem and The Aquaculture Built Environ...mentioning

confidence: 99%

Aquaculture ecosystem microbiome at the water-fish interface: the case-study of rainbow trout fed with Tenebrio molitor novel diets

Bruno,

Sandionigi,

Panio

et al. 2023

BMC Microbiol

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Background Sustainable aquaculture relies on multiple factors, including water quality, fish diets, and farmed fish. Replacing fishmeal (FM) with alternative protein sources is key for improving sustainability in aquaculture and promoting fish health. Indeed, great research efforts have been made to evaluate novel feed formulations, focusing especially on the effects on the fish gut microbiome. Few studies have explored host-environment interactions. In the present study, we evaluated the influence of novel insect-based (Tenebrio molitor) fish diets on the microbiome at the water-fish interface in an engineered rainbow trout (Oncorhynchus mykiss) farming ecosystem. Using 16S rRNA gene metabarcoding, we comprehensively analyzed the microbiomes of water, tank biofilm, fish intestinal mucus, fish cutis, and feed samples. Results Core microbiome analysis revealed the presence of a highly reduced core shared by all sample sources, constituted by Aeromonas spp., in both the control and novel feed test groups. Network analysis showed that samples were clustered based on the sample source, with no significant differences related to the feed formulation tested. Thus, the different diets did not seem to affect the environment (water and tank biofilm) and fish (cutis and intestinal mucus) microbiomes. To disentangle the contribution of feed at a finer scale, we performed a differential abundance analysis and observed differential enrichment/impoverishment in specific taxa, comparing the samples belonging to the control diet group and the insect-based diet group. Conclusions Omic exploration of the water-fish interface exposes patterns that are otherwise undetected. These data demonstrate a link between the environment and fish and show that subtle but significant differences are caused by feed composition. Thus, the research presented here is a step towards positively influencing the aquaculture environment and its microbiome.

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Section: A Focus On Water Ecosystem and The Aquaculture Built Environ...mentioning

confidence: 99%

Aquaculture ecosystem microbiome at the water-fish interface: the case-study of rainbow trout fed with Tenebrio molitor novel diets

Bruno,

Sandionigi,

Panio

et al. 2023

BMC Microbiol

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“…de/ genus/ chryseobacterium), and most species could form yellow colonies on solid medium. Species of Chryseobacterium were isolated from varied environments, such as terrestrial and aquatic environments [2][3][4][5], even including organisms and food [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant

Zhang

Guo

Kahaer

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, yellow-pigmented bacterium, designated as L7T, was isolated from seeds of Alhagi sparsifolia Shap., a leguminous plant that grows in northwest PR China. Strain L7T was found to be non-flagellated, non-spore forming rods which can grow at 10–37 °C, pH 6.0–8.5 and in 0–3 % (v/w) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain L7T belongs to the genus Chryseobacterium with sequence similarities to Chryseobacterium vietnamense GIMN1.005T (98.1%), C. bernardetii NCCTC13530T (98.0%), C. vrystaatense LMG 22846T (97.9%), C. nakagawai NCTC13529T (97.7%), C. shigense DSM 17126T (97.6%) and C. rhizosphaerae RSB3-1T (97.5%). The average nucleotide identity of strain L7T to 31 reference strains were 78.6–85.6 %, lower than the species delineation threshold of 95 %. MK-6 was the only respiratory quinone of L7T and major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 1 ω7c and/or C16 : 1 ω6c, isoC17 : 1 ω9c and/or C16 : 0 10-methyl. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, two unidentified aminolipids, three unidentified glycolipids and two unidentified lipids. The G+C content of the genome was 38.58 mol%. On the basis of polyphasic taxonomy analyses in this study, strain L7T is considered to represent a novel species in the genus Chryseobacterium , for which the name Chryseobacterium endalhagicum sp. nov. is proposed. The type strain is L7T (=MCCC 1K05687T=JCM 34506T)

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“…The genus is considered a part of the normal environmental microflora and has been associated with human infections [1,2]. Recently, several novel species have been isolated from raw milk samples, including C. mulctrae KACC 21234 T [3][4][5][6]. And although Chryseobacterium is not part of the normal microflora of milk, contamination can occur during the milking process (i.e., from cow udder, human handling, and immediate environment) or transport and processing [7].…”

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confidence: 99%

“…C. mulctrae KACC 21234 T was isolated based on its proteolytic activity on skim milk agar plate (SMA; 5% skim milk), incubated at 10℃ for 10 days. Strain KACC 21234 T was routinely cultured in tryptic soy agar (TSA, BD Difco, USA) at 30℃ [6]. The genomic DNA was extracted using QIAamp PowerFecal DNA kit (Qiagen, Germany) and sent to ChunLab (Korea) for sequencing using PacBio RSII Single Molecule Real-Time (SMRT) platform with 20 kb SMRTbell TM template library.…”

mentioning

confidence: 99%