Chryseobacterium angstadtii sp. nov., isolated from a newt tank

Abstract

As part of an undergraduate microbiology course, a yellow-orange-pigmented, Gram-staining negative, rod-shaped, non-motile bacterial strain was isolated from a glass tank housing several red-spotted newts (Notophthalmus viridescens). The sequence of the 16S rRNA gene of this strain, designated KM T , was 97.4-98.0 % similar to those of the type strains of Chryseobacterium luteum, C. shigense and C. vrystaatense, while the similarity levels for protein-coding genes were less than 94.7 % for rpoB, less than 92.1 % for groEL and less than 87.1 % for gyrB. These values are lower than for many other established distinct species. Polyphasic characterization and comparison to these relatives revealed that strain KM T was similar to other Chryseobacterium strains in that it contained MK-6 as its major respiratory quinone and phosphatidylethanolamine as the most abundant polar lipid, produced flexirubin-type pigments, oxidase and catalase and primarily contained the fatty acids iso-C 15 : 0 , iso-C 17 : 1 v9c, iso-C 17 : 0 3-OH and summed feature 3 (comprising C 16 : 1 v6c and/or C 16 : 1 v7c). Based on the results of this study, strain KM T represents a novel species, for which the name Chryseobacterium angstadtii sp. nov. is proposed. The type strain is KM T (5ATCC

Chryseobacterium frigidum sp. nov., isolated from high-Arctic tundra soil, and emended descriptions of Chryseobacterium bernardetii and Chryseobacterium taklimakanense

“…Members of the genus Chryseobacterium, described by Vandamme et al (1994), have been isolated from a wide variety of samples, such as the plant rhizosphere (Park et al, 2006;Kämpfer et al, 2014), compost (Kämpfer et al, 2010), soil (Shen et al, 2005;Weon et al, 2008;Hoang et al, 2013), meat (de Beer et al, 2005;Charimba et al, 2013), a newt tank (Kirk et al, 2013), desert soil (Peng et al, 2009), milk (Hantsis-Zacharov et al, 2008), diseased fish (Bernardet et al, 2005;Ilardi et al, 2009), a beer-bottling plant (Herzog et al, 2008), fresh water (Kim et al, 2005, a dairy environment (Hugo et al, 2003), wastewater (Kämpfer et al, 2003) and clinical samples (Vaneechoutte et al, 2007;Kämpfer et al, 2009). The members of the genus Chryseobacterium are Gramnegative and aerobic.…”

Chryseobacterium arachidiradicis sp. nov., isolated from the geocarposphere (soil around the peanut) of very immature peanuts (Arachis hypogaea)

“…Within the last few years, several novel species of the genus Chryseobacterium have been proposed (e.g. Hugo et al , 2003; Kämpfer et al , 2003; 2009, 2010a, b, 2011; Li et al , 2003; de Beer et al , 2005; Kim et al , 2005, 2008; Shen et al , 2005; Shimomura et al , 2005; Young et al , 2005; Park et al , 2006; Tai et al , 2006; Behrendt et al , 2007, 2008; Hantsis-Zacharov et al , 2008; Herzog et al , 2008; Szoboszlay et al , 2008; Weon et al , 2008; Cho et al 2010), with more than 20 species proposed since 2013 (Bajerski et al , 2013; Charimba et al , 2013; Hoang et al , 2013; Holmes et al , 2013; Kirk et al , 2013; Montero-Calasanz et al , 2013, 2014; Nguyen et al , 2013; Park et al , 2013; Sang et al , 2013; Wu et al , 2013; Kämpfer et al , 2014a, b, 2015; Kook et al , 2014; Loch & Faisal, 2014; Venil et al , 2014; Chen et al , 2015; Zhao et al , 2015). Among these species, several were found in close association with plants (Nguyen et al , 2013; Sang et al , 2013; Chen et al , 2014; Kämpfer et al , 2014a, 2015; Montero-Calasanz et al , 2014; Venil et al , 2014; Zhao et al , 2015).…”

Chryseobacterium solani sp. nov., isolated from field-grown eggplant rhizosphere soil

“…The genus Chryseobacterium , which belongs to the family Flavobacteriaceae , was first established by Vandamme et al (1994). Subsequently, the genus expanded rapidly to encompass more than 85 species, isolated from various environments such as soils (Venil et al , 2014), clinical samples (Holmes et al , 2013), fresh water (Kirk et al , 2013), sewage and wastewater (Kämpfer et al , 2003), plants (Sang et al , 2013), fish (Loch & Faisal, 2014) and meat (de Beer et al, 2005). The typical characteristics of the genus Chryseobacterium include aerobic type of metabolism, production of flexirubin-type pigments, the presence of branched-chain fatty acids (iso-C 15 : 0 and iso-C 17 : 0 3-OH) as the major fatty acids, phosphatidylethanolamine as a major polar lipid, and menaquinone-6 (MK-6) as the characteristic respiratory quinone (Bernardet et al , 2006, 2011; Im et al 2011; Kämpfer et al , 2009; Park et al , 2006; Vandamme et al , 1994; Wu et al , 2013).…”