Chronological in vivo imaging reveals endothelial inflammation prior to neutrophils accumulation and lipid deposition in HCD-fed zebrafish

Luo, Hui; Li, Qi-qi; Wu, Na; Shen, Yuge; Liao, Wei‐Ting; Yang, Yun; Dong, Edward; Zhang, Guimin; Liu, Binrui; Yue, Xiaozhu; Tang, Xiaoqiang; Yang, Huiling

doi:10.1016/j.atherosclerosis.2019.09.017

Cited by 19 publications

(14 citation statements)

References 53 publications

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“…They were able to show a co-localization of vascular lipid deposits with macrophages in apoc2 −/− mpeg1-eGFP 14 dpf as well as IK17 co-localization in selected areas of the larval fatty streaks, which is observed in early atherosclerotic lesions in human and mice (28,100). In concordance other studies demonstrated a significant increase of LDL concentrations at vascular bifurcations (LDL concentration polarization) in vivo in larvae 52 hpf after fluorescent DiI-LDLinjection 48 hpf (5) and after feeding larvae fluorescence-labeled HCD short term (10 day) and long term (<10 weeks) (53).…”

Section: Zebrafishsupporting

confidence: 76%

“…Use of transgenic zebrafish lines that can track macrophages and neutrophils in vivo has recently provided new insights into the early development of atherosclerosis. Chronological in vivo imaging over a 10-day period revealed, that neutrophils rather than macrophages may accumulate in the sub-endothelial space before LDL concentration polarization (53). These results illustrate the importance of appropriate in vivo models for studying the pathophysiology of complex diseases such as atherosclerosis.…”

Section: Discussionmentioning

confidence: 68%

“…Studies of the atheroprotective characteristics of oxLDL antibodies, which were demonstrated in mice and rabbits, could also be performed in zebrafish, followed by immunization studies (53,119). Furthermore, functional analysis of chemokines in growing plaques and regulation of their promoters in zebrafish could further drive atherosclerosis research.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Dare to Compare. Development of Atherosclerotic Lesions in Human, Mouse, and Zebrafish

Vedder

Aherrahrou

Erdmann

2020

Front. Cardiovasc. Med.

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Cardiovascular diseases, such as atherosclerosis, are the leading cause of death worldwide. Although mice are currently the most commonly used model for atherosclerosis, zebrafish are emerging as an alternative, especially for inflammatory and lipid metabolism studies. Here, we review the history of in vivo atherosclerosis models and highlight the potential for future studies on inflammatory responses in lipid deposits in zebrafish, based on known immune reactions in humans and mice, in anticipation of new zebrafish models with more advanced atherosclerotic plaques.

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Section: Zebrafishsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dare to Compare. Development of Atherosclerotic Lesions in Human, Mouse, and Zebrafish

Vedder

Aherrahrou

Erdmann

2020

Front. Cardiovasc. Med.

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“…Mammalian models, such as mice and rabbits, and human biopsy samples, can provide only endpoint results (Lee et al, 2017a,b), while zebrafish can be used to monitor the development of AS dynamically using in vivo live imaging because of their embryonic transparence and an abundance of fluorescent reporter lines (Fang and Miller, 2012;Gut et al, 2017). Luo et al (2019) fed Tg(lysc:EGFP), Tg(mpx:EGFP), and Tg(mpeg1:EGFP) transgenic zebrafish lines HCD to dynamically monitor myeloid cells/neutrophils in vivo during the initial stage of atherosclerosis. They also enriched endothelial cells with green fluorescence from HCD fed Tg(flk1:EGFP) fish by flow cytometry to detect gene expression (Luo et al, 2019).…”

Section: Dynamic Monitoring Of As Progressionmentioning

confidence: 99%

Recent Application of Zebrafish Models in Atherosclerosis Research

Tang

Geng

et al. 2021

Front. Cell Dev. Biol.

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Atherosclerotic cardiovascular disease is one of the leading causes of death worldwide. Establishing animal models of atherosclerosis is of great benefit for studying its complicated pathogenesis and screening and evaluating related drugs. Although researchers have generated a variety of models for atherosclerosis study in rabbits, mice and rats, the limitations of these models make it difficult to monitor the development of atherosclerosis, and these models are unsuitable for large scale screening of potential therapeutic targets. On the contrast, zebrafish can fulfill these purposes thanks to their fecundity, rapid development ex utero, embryonic transparency, and conserved lipid metabolism process. Thus, zebrafish have become a popular alternative animal model for atherosclerosis research. In this mini review, we summarize different zebrafish models used to study atherosclerosis, focusing on the latest applications of these models to the dynamic monitoring of atherosclerosis progression, mechanistic study of therapeutic intervention and drug screening, and assessment of the impacts of other risk factors.

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“…HCD induces endothelial inflammation along with peroxisome proliferator-activated receptor-gamma ( pparγ ) downregulation prior to lipid accumulation and atherosclerosis. Treatment with an agonist to PPARγ ameliorated the phenotype [ 126 ]. A study using berbamine identified its anti-hypercholesterolemic and hepatoprotective effect [ 127 ].…”

Section: Lipoprotein Metabolismmentioning

confidence: 99%

A Great Catch for Investigating Inborn Errors of Metabolism—Insights Obtained from Zebrafish

Breuer

Patten

2020

Biomolecules

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Inborn errors of metabolism cause abnormal synthesis, recycling, or breakdown of amino acids, neurotransmitters, and other various metabolites. This aberrant homeostasis commonly causes the accumulation of toxic compounds or depletion of vital metabolites, which has detrimental consequences for the patients. Efficient and rapid intervention is often key to survival. Therefore, it requires useful animal models to understand the pathomechanisms and identify promising therapeutic drug targets. Zebrafish are an effective tool to investigate developmental mechanisms and understanding the pathophysiology of disorders. In the past decades, zebrafish have proven their efficiency for studying genetic disorders owing to the high degree of conservation between human and zebrafish genes. Subsequently, several rare inherited metabolic disorders have been successfully investigated in zebrafish revealing underlying mechanisms and identifying novel therapeutic targets, including methylmalonic acidemia, Gaucher’s disease, maple urine disorder, hyperammonemia, TRAPPC11-CDGs, and others. This review summarizes the recent impact zebrafish have made in the field of inborn errors of metabolism.

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Chronological in vivo imaging reveals endothelial inflammation prior to neutrophils accumulation and lipid deposition in HCD-fed zebrafish

Cited by 19 publications

References 53 publications

Dare to Compare. Development of Atherosclerotic Lesions in Human, Mouse, and Zebrafish

Dare to Compare. Development of Atherosclerotic Lesions in Human, Mouse, and Zebrafish

Recent Application of Zebrafish Models in Atherosclerosis Research

A Great Catch for Investigating Inborn Errors of Metabolism—Insights Obtained from Zebrafish

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