Chronically implantable LED arrays for behavioral optogenetics in primates

Rajalingham, Rishi; Sorenson, Michael; Azadi, Reza; Bohn, Simon; DiCarlo, James J.; Afraz, Arash

doi:10.1038/s41592-021-01238-9

Cited by 57 publications

(57 citation statements)

References 28 publications

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“…The micro technique used in this study was less invasive and had less effect on the natural behavior of mice. Now, optogenetics not only controls the behavior of rodents but also primates ( Rajalingham et al, 2021 ). These studies demonstrates the broad application of optogenetics in human social behavior circuits.…”

Section: Optogenetic Research Methodsmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

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Optogenetic is a technique that combines optics and genetics to control specific neurons. This technique usually uses adenoviruses that encode photosensitive protein. The adenovirus may concentrate in a specific neural region. By shining light on the target nerve region, the photosensitive protein encoded by the adenovirus is controlled. Photosensitive proteins controlled by light can selectively allow ions inside and outside the cell membrane to pass through, resulting in inhibition or activation effects. Due to the high precision and minimally invasive, optogenetics has achieved good results in many fields, especially in the field of neuron functions and neural circuits. Significant advances have also been made in the study of many clinical diseases. This review focuses on the research of optogenetics in the field of neurobiology. These include how to use optogenetics to control nerve cells, study neural circuits, and treat diseases by changing the state of neurons. We hoped that this review will give a comprehensive understanding of the progress of optogenetics in the field of neurobiology.

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Section: Optogenetic Research Methodsmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

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“…Taken together, the methods presented here serve as an accessible and inexpensive protocol to plan the optimized spread of infusions bench-side and validate the spread and accuracy in vivo, significantly reducing the number of unknowns that hinder confidence during circuit-manipulation experiments. Our presented methods contribute to a body of work supporting large-scale optogenetics in NHPs [ 8 , 9 , 16 , 17 , 18 , 19 , 22 , 23 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates

Griggs

Garcia

et al. 2022

Pharmaceutics

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Non-human primates (NHPs) are precious resources for cutting-edge neuroscientific research, including large-scale viral vector-based experimentation such as optogenetics. We propose to improve surgical outcomes by enhancing the surgical preparation practices of convection-enhanced delivery (CED), which is an efficient viral vector infusion technique for large brains such as NHPs’. Here, we present both real-time and next-day MRI data of CED in the brains of ten NHPs, and we present a quantitative, inexpensive, and practical bench-side model of the in vivo CED data. Our bench-side model is composed of food coloring infused into a transparent agar phantom, and the spread of infusion is optically monitored over time. Our proposed method approximates CED infusions into the cortex, thalamus, medial temporal lobe, and caudate nucleus of NHPs, confirmed by MRI data acquired with either gadolinium-based or manganese-based contrast agents co-infused with optogenetic viral vectors. These methods and data serve to guide researchers and surgical team members in key surgical preparations for intracranial viral delivery using CED in NHPs, and thus improve expression targeting and efficacy and, as a result, reduce surgical risks.

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“…In these studies, LFPs were simultaneously recorded using microelectrocorticograms (μECoG) with improved transparency for compatibility with optical stimulation. An implantable subdural LED array capable of light stimulation over a wide range of cortical areas has also been developed and used successfully to manipulate neural activity and confirm behavioral changes in the macaque visual cortex ( Rajalingham et al, 2021 ), whereas the delivery of light stimuli to deep cortical layers remains a challenge. These innovations may be particularly useful for less densely connected neural pathways, such as dopaminergic projections from the midbrain to the PFC ( Ott and Nieder, 2019 ).…”

Section: Strategies For Efficient Light and Ligand Delivery In The Ma...mentioning

confidence: 99%

Dissecting the Prefrontal Network With Pathway-Selective Manipulation in the Macaque Brain—A Review

Oguchi

Sakagami

2022

Front. Neurosci.

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Macaque monkeys are prime animal models for studying the neural mechanisms of decision-making because of their close kinship with humans. Manipulation of neural activity during decision-making tasks is essential for approaching the causal relationship between the brain and its functions. Conventional manipulation methods used in macaque studies are coarse-grained, and have worked indiscriminately on mutually intertwined neural pathways. To systematically dissect neural circuits responsible for a variety of functions, it is essential to analyze changes in behavior and neural activity through interventions in specific neural pathways. In recent years, an increasing number of studies have applied optogenetics and chemogenetics to achieve fine-grained pathway-selective manipulation in the macaque brain. Here, we review the developments in macaque studies involving pathway-selective operations, with a particular focus on applications to the prefrontal network. Pathway selectivity can be achieved using single viral vector transduction combined with local light stimulation or ligand administration directly into the brain or double-viral vector transduction combined with systemic drug administration. We discuss the advantages and disadvantages of these methods. We also highlight recent technological developments in viral vectors that can effectively infect the macaque brain, as well as the development of methods to deliver photostimulation or ligand drugs to a wide area to effectively manipulate behavior. The development and dissemination of such pathway-selective manipulations of macaque prefrontal networks will enable us to efficiently dissect the neural mechanisms of decision-making and innovate novel treatments for decision-related psychiatric disorders.

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Chronically implantable LED arrays for behavioral optogenetics in primates

Cited by 57 publications

References 28 publications

The Roles of Optogenetics and Technology in Neurobiology: A Review

The Roles of Optogenetics and Technology in Neurobiology: A Review

Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates

Dissecting the Prefrontal Network With Pathway-Selective Manipulation in the Macaque Brain—A Review

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