Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants

Liu, Jiankang; Yeo, Helen; Övervik-Douki, Eva; Hagen, Tory M.; Doniger, Stephanie J.; Chu, Daniel Wai‐Sing; Brooks, George A.; Ames, Bruce N.

doi:10.1152/jappl.2000.89.1.21

Cited by 371 publications

(341 citation statements)

References 41 publications

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“…Although we did not attempt to measure any biomarkers of oxidative stress, the increase in antioxidative gene expression found in the whole-genome-expression analysis (data not shown) suggests that our exercise protocol could have induced oxidative stress in the liver. Moreover, increased generation of reactive oxygen species in the liver of rats after acute exercise has been reported [32,33]. Therefore, we evaluated the effect of an antioxidant-rich diet on the effects of the single bout of exercise.…”

Section: Microarray Expression Analysismentioning

confidence: 98%

Activation of the mitogen-activated protein kinase (MAPK) signalling pathway in the liver of mice is related to plasma glucose levels after acute exercise

et al. 2010

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Aims/hypothesis We aimed to identify, in the liver of mice, signal transduction pathways that show a pronounced regulation by acute exercise. We also aimed to elucidate the role of metabolic stress in this response. Methods C57Bl6 mice performed a 60 min run on a treadmill under non-exhaustive conditions. Hepatic RNA and protein lysates were prepared immediately after running and used for whole-genome-expression analysis, quantitative real-time PCR and immunoblotting. A subset of mice recovered for 3 h after the treadmill run. A further group of mice performed the treadmill run after having received a vitamin C-and vitamin E-enriched diet over 4 weeks. Results The highest number of genes differentially regulated by exercise in the liver was found in the mitogenactivated protein kinase (MAPK) signalling pathway, with a pronounced and transient upregulation of the transcription factors encoded by c-Fos (also known as Fos), c-Jun (also known as Jun), FosB (also known as Fosb) and JunB (also known as Junb) and phosphorylation of hepatic MAPK. Acute exercise also activated the p53 signalling pathway. A major role for oxidative stress is unlikely since the antioxidant-enriched diet did not prevent the activation of the MAPK pathway. In contrast, lower plasma glucose levels after running were related to enhanced levels of MAPK signalling proteins, similar to the upregulation of Igfbp1 and Pgc-1α (also known as Ppargc1a). In the working muscle the activation of the MAPK pathway was weak and not related to plasma glucose concentrations. Conclusions/interpretation Metabolic stress evidenced as low plasma glucose levels appears to be an important determinant for the activation of the MAPK signalling pathway and the transcriptional response of the liver to acute exercise.

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Section: Microarray Expression Analysismentioning

confidence: 98%

Activation of the mitogen-activated protein kinase (MAPK) signalling pathway in the liver of mice is related to plasma glucose levels after acute exercise

et al. 2010

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“…Leeuwenburgh et al (37) and Radák et al (38) found increased levels of oxidized amino acids in muscle mitochondria but not in cytosolic proteins, suggesting that exercise is a physiological source of oxidative stress. It has also been suggested that oxidative injury might cause irreversible cell damage through the loss of homeostasis and the loss of mitochondrial function (39). Given the high oxygen consumption during exercise, mitochondria would be requested for energy supplying and might simultaneously produce ROS.…”

Section: Complexesmentioning

confidence: 99%

Evaluation by blue native polyacrylamide electrophoresis colorimetric staining of the effects of physical exercise on the activities of mitochondrial complexes in rat muscle

Molnár

Alves

Pereira-Da-Silva

et al. 2004

Braz J Med Biol Res

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Blue native polyacrylamide electrophoresis (BN-PAGE) is a technique developed for the analysis of membrane complexes. Combined with histochemical staining, it permits the analysis and quantification of the activities of mitochondrial oxidative phosphorylation enzymes using whole muscle homogenates, without the need to isolate muscle mitochondria. Mitochondrial complex activities were measured by emerging gels in a solution containing all specific substrates for NADH dehydrogenase and cytochrome c oxidase enzymes (complexes I and IV, respectively) and the colored bands obtained were measured by optique densitometry. The objective of the present study was the application of BN-PAGE colorimetric staining for enzymatic characterization of mitochondrial complexes I and IV in rat muscles with different morphological and biochemical properties. We also investigated these activities at different times after acute exercise of rat soleus muscle. Although having fewer mitochondria than oxidative muscles, white gastrocnemius muscle presented a significantly higher activity (26.7 ± 9.5) in terms of complex I/V ratio compared to the red gastrocnemius (3.8 ± 0.65, P < 0.05) and soleus (9.8 ± 0.9, P < 0.001) muscles. Furthermore, the complex IV/V ratio of white gastrocnemius muscle was always significantly higher when compared to the other muscles. Ninety-five minutes of exhaustive physical exercise induced a decrease in complex I/V and complex IV/V ratios after all resting times (0, 3 and 6 h) compared to control (P < 0.05), probably reflecting the oxidative damage due to increasing free radical production in mitochondria. These results demonstrate the possible and useful application of BN-PAGE-histochemical staining to physical exercise studies. Correspondence

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“…On the other hand, Liu et al 30 verified, after eight weeks of training on a conveyor-belt, higher levels of MDA in the cardiac and vatus lateralis muscles, after 48 hours of the last training session in female mice. However, the same result was not observed after exhaustive exercise.…”

Section: Discussionmentioning

confidence: 98%

Evaluation of oxidative stress in mice subjected to aerobic exercise

Lima¹,

Marks²,

Silva³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To evaluate the influence of aerobic exercise on oxidative stress in mice. METHODS:The study included twenty female mice Mus musculus-Swiss divided into two groups: sedentary control (GA) and exercise (GB), each containing ten animals. All animals underwent an adaptation period of seven days isolated in individual boxes. After this period, the animals in the exercise group (GB) were trained in angled running wheel with circumference of 25 cm assembled on an articulated axle during five minutes for three consecutive days. On the fourth day, they underwent an exercise program of one session lasting 45 minutes. The evaluation of oxidative stress was performed by determining the levels of malondialhyde derived of lipid peroxidation by the TBA method. The samples were read in a spectrophotometer at 535 nm. RESULTS: No significant difference was observed in the intergroup comparison of MDA levels in the tissues evaluated. A significant difference was observed in the intragroup comparison of MDA levels in the control group (p = 0.0201).The Tukeys' post hoc test indicated significantly lower values of MDA in the smooth muscle in relation to plasma. In the analysis of variance in the exercise group, a significant difference between tissues (p = 0.0009), with significantly lower values in the smooth muscle in relation to plasma (p<0.001) and higher in striated muscle in relation to smooth muscle (p<0.05) was observed. CONCLUSION: There was no change in the analysis of oxidative stress in mice which were undergone a single session of aerobic exercise. Key words: Oxidative Stress. Exercise. Lipids. Mice. RESUMO OBJETIVO:Avaliar a influência do exercício físico aeróbico sobre o estresse oxidativo em camundongos. MÉTODOS: Participaram do estudo 20 camundongos (Swiss), distribuídos em dois grupos: controle-sedentário (GA) e exercício (GB) cada um contendo dez animais. Todos os animais passaram por um período de adaptação de sete dias. Após os animais do grupo (GB) receberam treinamento em roda giratória angulada montada sobre eixo articulado por cinco minutos durante três dias consecutivos. No quarto dia foram submetidos à única sessão de exercício por 45 minutos. A avaliação do estresse oxidativo foi realizada por meio dos níveis de malondiadeído pelo método do TBA. As amostras foram lidas em espectrofotômetro a 535nm. RESULTADOS: Não houve diferença significativa na comparação intergrupos nos tecidos avaliados. Diferença significativa foi observada na comparação intragrupo para o GA (p=0,0201). O post hoc test de Tukey apontou valores significantemente inferiores no músculo liso em relação ao plasma. A análise de variância do GB apontou diferença significativa entre os tecidos (p=0,0009), com valores menores no músculo liso em relação ao plasma (p<0,001), e maiores no músculo estriado em relação ao músculo liso (p<0,05). CONCLUSÃO: Não houve alteração nas análises de malondialdeído tecidual entre os grupos avaliados. Descritores: Estresse Oxidativo. Exercício. Lipídeos. Camundongos. Evaluation of oxidative s...

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Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants

Cited by 371 publications

References 41 publications

Activation of the mitogen-activated protein kinase (MAPK) signalling pathway in the liver of mice is related to plasma glucose levels after acute exercise

Activation of the mitogen-activated protein kinase (MAPK) signalling pathway in the liver of mice is related to plasma glucose levels after acute exercise

Evaluation by blue native polyacrylamide electrophoresis colorimetric staining of the effects of physical exercise on the activities of mitochondrial complexes in rat muscle

Evaluation of oxidative stress in mice subjected to aerobic exercise

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