Chronic Stress Disturbs Metabolome of Blood Plasma and Urine in Diabetic Rats

Abstract

About 30% of diabetes patients suffer from varying degrees of depression. Diabetes itself is associated with abnormal carbohydrate and energy metabolism. Whether chronic stress-induced depression-like behavior impacts the metabolome of blood plasma and urine in diabetes is not clear. This study aimed to investigate the effect of chronic stress on metabolome of plasma and urine in spontaneously diabetic Goto-Kakizaki (GK) rats. The GK rats were subjected to 8 weeks' chronic unpredictable mild stress (CUMS) to induce depression-like behavior. Metabolome analysis of blood plasma and urine using liquid chromatography mass spectrometry (LC/MS) was performed. Multivariate data analysis was used to evaluate the data. Behavior and biochemical assay confirmed the successful establishment of CUMS-induced depression-like behavior model in rats. Disturbance of 20 plasma metabolites and 16 urine metabolites were altered in CUMS-induced depression GK rats as compared to control ones. These disturbed metabolites were involved in fatty acid metabolism, sphingolipid metabolism, phenylalanine metabolism, citrate cycle, glycolysis, glutathione metabolism, and nicotinate and nicotinamide metabolism. This study suggest that chronic stress-induced depression-like behavior may further disturb diabetes-itself energy metabolome. The plasma and urine lipid metabolites monitoring may be useful for early detection of depression in patients with diabetes mellitus.

Jujuboside B Reverse CUMS-Promoted Tumor Progression via Blocking PI3K/Akt and MAPK/ERK and Dephosphorylating CREB Signaling

“…Depression is a common comorbidity in cancer patients. In our previous work, we noted that about 30% of cancer patients occurred mild to major depression [ 3 , 4 ]. Many factors lead to comorbid depression in cancer patients.…”

Antitumor Effect of Fluoxetine on Chronic Stress-Promoted Lung Cancer Growth via Suppressing Kynurenine Pathway and Enhancing Cellular Immunity

“…Fluoxetine, a selective serotonin reuptake inhibitor, is a first choice for patients with depression. After fluoxetine treatment, symptoms of depression and the quality of life are significantly improved ( Lieb, 2008 ; Ren et al, 2018 ). Fortunately, fluoxetine inhibits cancer cells growth including oral, glioma, rectal, and hepatocellular carcinoma cancer cells ( Peer et al, 2004 ; Kannen et al, 2015 ; Liu et al, 2015 ; Chen et al, 2019 ).…”

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Anxiety disturbs the blood plasma metabolome in acute coronary syndrome patients

“…UPLC-QTOF-MS analyzer (Impact II; Bruker) was used to analyze the samples. The chromatographic separation conditions and quality control (QC) procedures were based on the experimental methods of Ren et al 28 . Briefly, each sample (10 µL) was injected into an Acclaim TM 120 C 18 column (Thermo Fisher, USA, 100 × 2.1 mm, 2.2 µm) at 4 °C, and the following mobile phases were used at a flow rate of 0.2 mL/min at 30 °C: H 2 O with 0.1% methane acid (A) and acetonitrile with 0.1% methane acid (B).…”