Chronic shisha exposure alters phosphoproteome of oral keratinocytes

Patil, Shankargouda; Rajagopalan, Padmavathy; Patel, Krishna; Subbannayya, Tejaswini; Babu, Niraj; Mohan, Sonali V.; Advani, Jayshree; Sathe, Gajanan; Bhandi, Shilpa; Solanki, Hitendra S.; Sidransky, David; Chatterjee, Aditi; Gowda, Harsha; Ferrari, Marco

doi:10.1007/s12079-019-00528-4

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…After screen reading and excluding paper unrelated to our search, 38 remained. Reports from the same authors/co-authors or same study center were excluded [14][15][16][17][18][19] as such reviews, comments, letters, hypotheses, and expert opinions 3,[20][21][22][23][24][25][26] . Two exclusive microbiological studies were removed 27,28 , same to animal studies 29,30 .…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic and Genotoxic Effects of Waterpipe on Oral Health Status

Grillo

Khemiss

Silva

2023

Sultan Qaboos Univ Med J

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A worldwide increase in waterpipe consumption can be observed. The present systematic review aims to assess cytotoxic and genotoxic impacts on oral health related to waterpipe smoking. We searched MEDLINE, Cochrane Library, and Dimensions evaluating if waterpipe smokers (P) have any cytotoxic or genotoxic effects on oral cells (I) compared to non-smokers (C) regarding mouth neoplasms (O). PRISMA guidelines were adopted for the current systematic review. Review Manager was utilized for statistical analysis (p < 0.05). A risk of bias and summary were performed to assess the grade of the 20 included articles. With some of the articles included, a forest plot was created in different levels. Waterpipe smoking is harmful to oral health, causing cytotoxic and genotoxic effects on oral cells with a Risk Difference of 0.16. It causes a series of detrimental cellular and genetic modifications such as acanthosis, epithelial dysplasia, and hyperparakeratosis. Changes in DNA methylation and p53 expression were assessed among others. In addition, waterpipe has a bunch of carcinogenic compounds. Even with few publications on the subject, articles are very devastating in confirming the carcinogenicity of waterpipe smoking. Waterpipe smoke is cytotoxic and genotoxic. Due to the release of many organic compounds, it increases the incidence of oral cancer.Keywords: Mouth Neoplasms; Oral Health; Smoking Water Pipes; Tobacco Use; Toxicity Measure.

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Section: Resultsmentioning

confidence: 99%

Cytotoxic and Genotoxic Effects of Waterpipe on Oral Health Status

Grillo

Khemiss

Silva

2023

Sultan Qaboos Univ Med J

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“…Recently, a series of multiomic studies carried out by our group provided substantial evidence to indicate that chronic exposure to WS leads to oncogenic transformation in normal human oral keratinocytes. [5][6][7][8] The main component used in these studies is 'shisha'-a processed tobacco variant blended with flavors and additives.…”

Section: Introductionmentioning

confidence: 99%

Signaling alterations in oral keratinocytes in response to shisha and crude tobacco extract

Babu

Patil

Mohan

et al. 2021

J Oral Pathology Medicine

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Background: Tobacco consumption in smoking and non-smoking forms has been consequential in the rise of oral cancer cases. Among different forms, epidemiological studies from Middle Eastern countries and rural parts of northern India have reported increasing association of oral cancer with waterpipe (hookah) smoking. However, molecular mechanisms and role played by waterpipe smoking in the onset of oral carcinogenesis remains unexplored. Methods: In this study, immortalized normal human oral keratinocytes were chronically treated with extracts of two varieties of waterpipe tobacco-crude tobacco and processed shisha. Phenotypic changes and molecular aberrations were examined using cell culture-based assays and mass spectrometry-based quantitative proteomic analysis, respectively. Bioinformatics analysis was utilized to analyze proteomics data and identify dysregulated pathways. Results: Our data indicate that chronic treatment with waterpipe tobacco extracts increased proliferation, invasion, migration, and significant dysregulation of protein expression in oral keratinocytes. Altered expression of proteins involved in interferon signaling pathway were observed with both varieties of tobacco. Overexpression of cholesterol metabolism and vesicle-mediated transport proteins were identified exclusively in cells treated with crude tobacco extract. Bioinformatics analyses revealed different oncogenic response in oral cells based on the type of waterpipe tobacco used.Conclusions: This study may serve as a useful resource in understanding the early onset of oral cancer attributed to waterpipe smoking.

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