Chronic Mouse Model of TMA-Induced Contact Hypersensitivity

Schneider, Claudia; Döcke, Wolf‐Dietrich; Zollner, Thomas M.; Röse, Lars

doi:10.1038/jid.2008.307

Cited by 54 publications

(74 citation statements)

References 29 publications

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“…Interestingly, in our studies, IFN gamma, a representative Th1 cytokine, remained unaffected by PFE (data not shown). A previous study that established the chronic TMA rodent model also revealed that prednisolone does not affect TMA-induced IgE production, even though it has a suppressive effect on TMA-induced dermatitis (Schneider et al, 2009). We found that oral administration of PFE strongly suppressed TMA-induced IL-4 and IL-5 production, as well as IL-4 mRNA expression in vivo.…”

Section: Discussionmentioning

confidence: 48%

“…However, to date, the fruit of Piper nigrum and its biological effects on ACD remain unclear. Multiple lines of evidence suggest that rodent models are optimal for the development of anti-ACD materials because rodent dermatitis induced by haptens shares common symptoms with human dermatitis (Schneider et al, 2009). Therefore, we adopted a TMA-induced rodent dermatitis model and prednisolone as a positive control to investigate the effects of PFE on TMA-induced dermatitis (Schneider et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…To investigate the effects of PFE on allergic contact dermatitis (ACD), we conducted a TMA-induced chronic ACD study (Schneider et al, 2009). Prednisolone (PS) was used as a positive control.…”

Section: Effects Of Bpe On Tma-induced Ear Swelling and Increases In mentioning

confidence: 99%

“…Induction of CHS was achieved by modifying a chronic CHS model (Schneider et al, 2009). Briefly, 6-week-old mice were divided into four groups: naive group (naive), sham group (sham), PFE 50 mg/kg group (PFE 50), PFE 200 mg/kg group (PFE 200), and a 10 mg/kg prednisolone group (PS 10).…”

Section: Induction Of Contact Hypersensitivity and Experimental Schedulementioning

confidence: 99%

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Piper nigrum Fruit Extract Prevents TMA-Induced Allergic Contact Dermatitis by Regulating Th2 Cytokine Production

Jung¹,

Choi²,

Jung³

et al. 2015

JAS

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Allergic contact dermatitis (ACD) remains a leading skin disease in many countries. In this study, we investigated the preventive effect of Piper nigrum fruit extract (PFE) on trimellitic anhydride (TMA)-induced dermatitis and its potential mechanism of action. Oral administration of PFE and prednisolone (PS) significantly suppressed TMA-induced increases in ear, epidermal thickness, and infiltration of CD4 + cells, while abnormal expression of IgE, mMCP-1, IL-1β and IL-1β mRNA was also significantly counteracted by oral administration of PFE. PFE also significantly suppresses TMA-induced IL-4 and IL-5 production and IL-4 mRNA expression in vivo, as well as OVA-induced IL-4, IL-5, and IL-13 production and GATA3 mRNA expression ex vivo, and IL-4 induced STAT6 phosphorylation in primary cultured splenocytes and HaCaT cells. Interestingly, the PFE component piperine significantly suppressed OVA-induced IL-4, IL-5, and IL-13 secretion ex vivo. Taken together, these results suggest that PFE could be useful in suppressing allergic contact dermatitis.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Effects Of Bpe On Tma-induced Ear Swelling and Increases In mentioning

confidence: 99%

Section: Induction Of Contact Hypersensitivity and Experimental Schedulementioning

confidence: 99%

See 2 more Smart Citations

Piper nigrum Fruit Extract Prevents TMA-Induced Allergic Contact Dermatitis by Regulating Th2 Cytokine Production

Jung¹,

Choi²,

Jung³

et al. 2015

JAS

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show abstract

“…In atopic diseases such as asthma and atopic dermatitis, not only dendritic cells, epithelial cells, eosinophils, and T cells but also mast cells are important. A number of studies reported that mast cells are activated and infiltrated in the skin lesion of the atopic dermatitis animal model, suggesting the contribution of mast cells in atopic dermatitis [4,9,25,30]. The cysteine protease caspase-1 is a member of the caspase family [6].…”

Section: Introductionmentioning

confidence: 99%

Down-regulation of thymic stromal lymphopoietin by curcumin

Moon

Jeong

Kim

2013

Pharmacological Reports

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Abstract:Background: Thymic stromal lymphopoietin (TSLP) is a cytokine implicated in the pathogenesis of allergic diseases such as asthma, atopic dermatitis and allergic rhinitis. Curcumin has various effects such as antidepressant, antioxidant, antihyperglycemic, antitumor and anti-inflammatory. However, the effect of curcumin on the production of TSLP has not been clarified. Thus, we investigated how curcumin inhibits the expression and production of TSLP in the human mast cell line, HMC-1 cells. Methods: We used enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and caspase-1 assay to investigate the effects of curcumin. Results:The results show that curcumin inhibited the production and mRNA expression of TSLP in HMC-1 cells: the maximal inhibition rate of TSLP production by curcumin (50 µM) was 59.16 ± 4.20%. In addition, curcumin suppressed the nuclear factor-kB luciferase activity induced by phorbol myristate acetate plus A23187. In the activated HMC-1 cells, caspase-1 activity was increased, whereas caspase-1 activity was decreased by pretreatment with curcumin. Conclusion: These results suggest that curcumin can be used to treat inflammatory and atopic diseases through the suppression of TSLP.

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Involvement of Fas/FasL pathway in the murine model of atopic dermatitis

et al. 2017

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Objective and designThe aim of this study was to elucidate the role of apoptosis mediated through Fas/FasL pathway using the mouse model of atopic dermatitis (AD).Materials and treatment AD was induced by epicutaneous application of ovalbumin (OVA) in wild-type C57BL/6, B6. MRL-Faslpr/J (Fas−) and B6Smn.C3-Faslgld/J (FasL−) mouse strains.MethodsSkin samples were subjected to staining for Fas/FasL expression, M30 epitope and assessment of inflammatory response via immunohistochemical staining. Cytokine and chemokine production was assessed by real-time PCR.ResultsIn comparison to wild-type mice, OVA sensitization of Fas- and FasL-deficient mice led to increased epidermal and dermal thickness, collagen deposition and local inflammation consisting of macrophages, neutrophils and CD4+ T cells. Fas- and FasL-deficient mice showed increased total counts of regulatory T cells (Tregs) and IgE levels in blood as well as increased expression of IL-1β, IL-4, IL-5, IL-13 and TGF-1β mRNA in comparison to wild-type mice. On the other hand, expression of CXCL9 and CXCL10, IL-17 mRNAs in the skin samples in Fas- and FasL-deficient mice was decreased.ConclusionsOur results show that lack of the Fas-induced apoptosis leads to exacerbation of AD characteristics such as Th2 inflammation and dermal thickening. Therefore, Fas receptor can play an important role in AD pathogenesis by controlling development of the local inflammation.

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Chronic Mouse Model of TMA-Induced Contact Hypersensitivity

Cited by 54 publications

References 29 publications

Piper nigrum Fruit Extract Prevents TMA-Induced Allergic Contact Dermatitis by Regulating Th2 Cytokine Production

Piper nigrum Fruit Extract Prevents TMA-Induced Allergic Contact Dermatitis by Regulating Th2 Cytokine Production

Down-regulation of thymic stromal lymphopoietin by curcumin

Involvement of Fas/FasL pathway in the murine model of atopic dermatitis

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