Chronic infusion of berberine into the hypothalamic paraventricular nucleus attenuates hypertension and sympathoexcitation via the ROS/Erk1/2/iNOS pathway

Tian, Hua; Kang, Yu-Ming; Gao, Hong-Li; Shi, Xiao-Lian; Fu, Li-Yan; Liu, Ying; Jia, Xiuyue; Liu, Kaili; Qi, Jie; Li, Hong-Bao; Chen, Yanmei; Chen, Wensheng; Cui, Wei; Zhu, Guo‐Qing; Yu, Xiao-Jing

doi:10.1016/j.phymed.2018.09.206

Cited by 31 publications

(21 citation statements)

References 32 publications

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“…Transverse sections with thickness of 14∼18 μm were obtained from the region approximately 1.80 mm from the bregma of the brains. Immunohistochemistry and immunofluorescence studies were performed in floating sections as described previously (Tian et al, 2019). The primary antibodies for Fra-Li (sc-253, 1:100 dilution), TLR4 (sc-293072, 1:50 dilution), MyD88 (sc-74532, 1:50 dilution), and IL-1β (sc-52012, 1:50 dilution) were purchased from Santa Cruz Biotechnology, and TNF-α (ab6671, 1:20 dilution) was purchased from Abcam.…”

Section: Methodsmentioning

confidence: 99%

Exercise Training Attenuates Hypertension Through TLR4/MyD88/NF-κB Signaling in the Hypothalamic Paraventricular Nucleus

et al. 2019

Front. Neurosci.

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Exercise training (ExT) is beneficial for cardiovascular health, yet the central mechanism by which aerobic ExT attenuates the hypertensive responses remains unclear. Activation of pro-inflammatory cytokines (PICs) in the hypothalamic paraventricular nucleus (PVN) is important for the sympathoexcitation and hypertensive response. We thus hypothesized that aerobic ExT can decrease the blood pressure of hypertensive rats by reducing the levels of PICs through TLR4/MyD88/NF-κB signaling within the PVN. To examine this hypothesis, two-kidney-one-clip (2K1C) renovascular hypertensive rats were assigned to two groups: sedentary or exercise training and examined for 8 weeks. At the same time, bilateral PVN infusion of vehicle or TAK242, a TLR4 inhibitor, was performed on both groups. As a result, the systolic blood pressure (SBP), renal sympathetic nerve activity (RSNA) and plasma levels of norepinephrine (NE), epinephrine (EPI) were found significantly increased in 2K1C hypertensive rats. These rats also had higher levels of Fra-like activity, NF-κB p65 activity, TLR4, MyD88, IL-1β and TNF-α in the PVN than SHAM rats. Eight weeks of ExT attenuated the RSNA and SBP, repressed the NF-κB p65 activity, and reduced the increase of plasma levels of NE, EPI, and the expression of Fra-like, TLR4, MyD88, IL-1β and TNF-α in the PVN of 2K1C rats. These findings are highly similar to the results in 2K1C rats with bilateral PVN infusions of TLR4 inhibitor (TAK242). This suggests that 8 weeks of aerobic ExT may decrease blood pressure in hypertensive rats by reducing the PICs activation through TLR4/MyD88/NF-κB signaling within the PVN, and thus delays the progression of 2K1C renovascular hypertension.

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Section: Methodsmentioning

confidence: 99%

Exercise Training Attenuates Hypertension Through TLR4/MyD88/NF-κB Signaling in the Hypothalamic Paraventricular Nucleus

et al. 2019

Front. Neurosci.

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“…The antihypertensive activity of berberine is due to inhibiting the activity of cholinesterase, thus activating the M-receptor on vascular endothelial cells and promoting the release of the vasodilator NO from endothelial cells, which results in peripheral vascular smooth muscle relaxation. This mechanism may also contribute to the antioxidant effect of berberine [ 110 , 111 ]. In addition, it has been reported that berberine can regulate AMPK signaling and inhibit the overexpression of p-mTOR.…”

Section: Application Of Antioxidant Natural Drugs For Cardiovasculmentioning

confidence: 99%

Natural Drugs as a Treatment Strategy for Cardiovascular Disease through the Regulation of Oxidative Stress

Chang

Zhang

et al. 2020

Oxidative Medicine and Cellular Longevity

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Oxidative stress (OS) refers to the physiological imbalance between oxidative and antioxidative processes leading to increased oxidation, which then results in the inflammatory infiltration of neutrophils, increased protease secretion, and the production of a large number of oxidative intermediates. Oxidative stress is considered an important factor in the pathogenesis of cardiovascular disease (CVD). At present, active components of Chinese herbal medicines (CHMs) have been widely used for the treatment of CVD, including coronary heart disease and hypertension. Since the discovery of artemisinin for the treatment of malaria by Nobel laureate Youyou Tu, the therapeutic effects of active components of CHM on various diseases have been widely investigated by the medical community. It has been found that various active CHM components can regulate oxidative stress and the circulatory system, including ginsenoside, astragaloside, and resveratrol. This paper reviews advances in the use of active CHM components that modulate oxidative stress, suggesting potential drugs for the treatment of various CVDs.

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“…Specifically, the rapid clearance of BBR from plasma as compared to the hippocampus indicates that BBR may have an important effect on hippocampal neurons 31. Moreover, infusion of BBR (2 μg/h, 28d) by bilateral hypothalamic paraventricular nucleus (PVN) through an osmotic minipump can reduce hypertension and sympathoexcitation in two-kidney, one-clip (2K1C) renovascular hypertensive rats by ROS/ERK1/2 (extracellular-signal regulated kinase 1/2)/ inducible nitric oxide (iNOS) pathway 33.…”

Section: Bioavailability and Metabolism Of Bbrmentioning

confidence: 99%

Berberine in Cardiovascular and Metabolic Diseases: From Mechanisms to Therapeutics

et al. 2019

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Cardiovascular and metabolic diseases (CVMD) are the leading causes of death worldwide, underscoring the urgent necessity to develop new pharmacotherapies. Berberine (BBR) is an eminent component of traditional Chinese and Ayurvedic medicine for more than 2000 years. Recently, BBR has attracted much interest for its pharmacological actions in treating and/or managing CVMD. Recent discoveries of basic, translational and clinical studies have identified many novel molecular targets of BBR (such as AMPK, SIRT1, LDLR, PCSK9, and PTP1B) and provided novel evidences supporting the promising therapeutic potential of BBR to combat CVMD. Thus, this review provides a timely overview of the pharmacological properties and therapeutic application of BBR in CVMD, and underlines recent pharmacological advances which validate BBR as a promising lead drug against CVMD.

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Chronic infusion of berberine into the hypothalamic paraventricular nucleus attenuates hypertension and sympathoexcitation via the ROS/Erk1/2/iNOS pathway

Cited by 31 publications

References 32 publications

Exercise Training Attenuates Hypertension Through TLR4/MyD88/NF-κB Signaling in the Hypothalamic Paraventricular Nucleus

Exercise Training Attenuates Hypertension Through TLR4/MyD88/NF-κB Signaling in the Hypothalamic Paraventricular Nucleus

Natural Drugs as a Treatment Strategy for Cardiovascular Disease through the Regulation of Oxidative Stress

Berberine in Cardiovascular and Metabolic Diseases: From Mechanisms to Therapeutics

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