Chronic Inflammation And Neutrophil Activation As Possible Causes Of Joint Diseases In Ballet Dancers

Abstract

Herein, we investigated the effects of a ballet class on the kinetic profiles of creatine kinase (CK) and lactate dehydrogenase (LDH) activities, cytokines, complement component 3 (C3), and the concentrations of immunoglobulin (Ig), IgA and IgM, in ballerinas. We also verified neutrophil death and ROS release. Blood samples were taken from 13 dancers before, immediately after, and 18 hours after a ballet class. The ballet class increased the plasma activities of CK-total (2.0-fold) immediately after class, while the activities of CK-cardiac muscle (1.0-fold) and LDH (3.0-fold) were observed to increase 18 hours after the class. Levels of the TNF-, IL-1 , IgG, and IgA were not affected under the study conditions. The exercise was found to induce neutrophil apoptosis (6.0-fold) 18 hours after the ballet class. Additionally, immediately after the ballet class, the neutrophils from the ballerinas were found to be less responsive to PMA stimulus. Conclusion. Ballet class was found to result in inflammation in dancers. The inflammation caused by the ballet class remained for 18 hours after the exercise. These findings are important in preventing the development of chronic lesions that are commonly observed in dancers, such as those with arthritis and synovitis.

Assessment of female ballet dancers’ ankles in theen pointeposition using high field strength magnetic resonance imaging

“…It appears to be somewhat joint dependent and, predictably, age dependent. Plasma inflammatory markers were present in dancers immediately through 18 h following a classical ballet dance class (16), suggesting that participation in ballet is sufficiently intense as to stimulate an immunological response that could adversely affect joint cartilage. Evidence of OA was found in several joints of the lower extremity following MRI of professional ballet dancers (17).…”

“…The lack of osteoarthritic changes on imaging in young dancers also may be a function of subclinical microtraumatic changes in joint cartilage that are not seen on early imaging, are compounded over time, and must progress sufficiently to eventually become apparent on imaging (22). Of additional concern is a postulated chronic inflammation with neutrophil activation in dancers that may adversely affect joint cartilage (16).…”

Moderate physical training attenuates perinatal low-protein-induced spleen lymphocyte apoptosis in endotoxemic adult offspring rats

“…Spleen lymphocytes (2 × 10 6 ) were incubated for 30 min in a dark room in the presence of a solution containing the fluorochrome rhodamine 123 (2.6 M) in PBS [31]. Rhodamine 123 is a cell permeant, cationic, fluorescent dye that is sequestered by active mitochondria without inducing cytotoxic effects [32]. Cells with fluorescent rhodamine were then evaluated by flow cytometry (511/534 nm).…”

Recovery of Diabetic Rats After Physical Exhaustion: Kinetic Alterations in Muscle Inflammation and Muscle-Signaling Proteins to Atrophy and Hypertrophy

“…The cytokine levels of CINC-2α/β, IL-1β, TNF-α, IL-6, and L-selectin) were evaluated by ELISA (Duo Set kit, R&D System) as described by previous studies ( Borges et al, 2014 , 2019 ).…”