Chronic exposure to glyphosate induces transcriptional changes in honey bee larva: A toxicogenomic study

Vázquez, Diego E.; Latorre-Estivalis, José Manuel; Ons, Sheila; Farina, Walter M.

doi:10.1016/j.envpol.2020.114148

Cited by 44 publications

(40 citation statements)

References 70 publications

(92 reference statements)

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“…Studies in both vertebrates and invertebrates proved that GLY induces signs of metabolic stress 27,[40][41][42] . In honey bees, different studies have suggested that chronic exposures to this herbicide can trigger oxidative stress www.nature.com/scientificreports/ and detoxifying pathways in adults as well as in brood 27,[43][44][45][46] . Oxidative stress is associated with increased production of free radicals during catabolism and can trigger apoptosis and energy depletion 46,47 .…”

Section: Discussionmentioning

confidence: 99%

“…One of the widest agrochemicals used worldwide is the herbicide glyphosate (GLY) 25 . GLY is considered as a low toxicity pesticide for honey bees, however, research has shown that it affects their behaviour and physiology 26,27 . As we mentioned, sleep plays an essential role in honey bee memory consolidation 8,18,28 and there is evidence that GLY in chronic exposures impairs the associative learning and cognitive abilities of these pollinators 26 .…”

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confidence: 99%

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Sleep in honey bees is affected by the herbicide glyphosate

Vázquez

Balbuena

Chaves

et al. 2020

Sci Rep

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Sleep plays an essential role in both neural and energetic homeostasis of animals. Honey bees (Apis mellifera) manifest the sleep state as a reduction in muscle tone and antennal movements, which is susceptible to physical or chemical disturbances. This social insect is one of the most important pollinators in agricultural ecosystems, being exposed to a great variety of agrochemicals, which might affect its sleep behaviour. The intake of glyphosate (GLY), the herbicide most widely used worldwide, impairs learning, gustatory responsiveness and navigation in honey bees. In general, these cognitive abilities are linked with the amount and quality of sleep. Furthermore, it has been reported that animals exposed to sleep disturbances show impairments in both metabolism and memory consolidation. Consequently, we assessed the sleep pattern of bees fed with a sugar solution containing GLY (0, 25, 50 and 100 ng) by quantifying their antennal activity during the scotophase. We found that the ingestion of 50 ng of GLY decreased both antennal activity and sleep bout frequency. This sleep deepening after GLY intake could be explained as a consequence of the regenerative function of sleep and the metabolic stress induced by the herbicide.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Sleep in honey bees is affected by the herbicide glyphosate

Vázquez

Balbuena

Chaves

et al. 2020

Sci Rep

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“…Realistic field exposures are complex to carry out experimentally because there are many concomitant factors during the pesticide administration, as the effect of the combination of different substances (a mixture of agrochemical formulations) and low concentrations of residues mainly detectable close to the administration day [9,35]. Hence, measurements of the internal physiological state of animals with biomarkers of response are recommended in open field assessments to reveal signs of stress without conspicuous symptoms [36]. At the molecular level, exposure to pesticides can activate detoxification pathways in honeybees [36][37][38][39][40][41] and modulate the expression of genes involved in immunity and behavioral maturation [40,42].…”

Section: Introductionmentioning

confidence: 99%

“…Hence, measurements of the internal physiological state of animals with biomarkers of response are recommended in open field assessments to reveal signs of stress without conspicuous symptoms [36]. At the molecular level, exposure to pesticides can activate detoxification pathways in honeybees [36][37][38][39][40][41] and modulate the expression of genes involved in immunity and behavioral maturation [40,42]. The herbicides GLY and atrazine promoted lipid peroxidation [43] and altered acetylcholinesterase activity [44] and the carotenoid-retinoid system in honeybees [45].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings

Macri

Vázquez

Pagano

et al. 2021

Insects

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The honeybee Apis mellifera is exposed to agricultural intensification, which leads to an improved reliance upon pesticide use and the reduction of floral diversity. In the present study, we assess the changes in the colony activity and the expression profile of genes involved in xenobiotic detoxification in larvae and adult honeybees from three apiaries located in agricultural environments that differ in their proportion of the crop/wild flora. We evaluated these variables before and after the administration of a mixture of three herbicides during the summer season. The expression of several cytochrome P450 monooxygenases decreased significantly in larvae after post-emergence weed control and showed significant differences between apiaries in the case of honeybee workers. Principal component analysis (PCA) revealed that colonies located in the plot near to a wetland area exhibited a different relative gene expression profile after herbicide application compared with the other plots. Moreover, we found significant positive correlations between pollen collection and the pesticide detoxification genes that discriminated between plots in the PCA. Our results suggest that nutrition may modify herbicide impact on honeybees and that larvae are more harmed than adults in agroecosystems, a factor that will alter the colonies’ population growth at the end of the blooming period.

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“…Other sub-lethal effects on honeybee performance indicators such as reproduction [ 17 ] and larval development [ 25 ] have also been described when honeybees are exposed to pesticides. In the case of the herbicide glyphosate, topical contact impairs the ability of honeybees to return to their colonies [ 15 ], and affects the transcriptional levels of several genes [ 26 ]. Synthetic and natural acaricides, including thymol and formic acid usually used in organic practices, also affect the memory of honeybees [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Sub-lethal effects of the consumption of Eupatorium buniifolium essential oil in honeybees

et al. 2020

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When developing new products to be used in honeybee colonies, further than acute toxicity, it is imperative to perform an assessment of risks, including various sublethal effects. The long-term sublethal effects of xenobiotics on honeybees, more specifically of acaricides used in honeybee hives, have been scarcely studied, particularly so in the case of essential oils and their components. In this work, chronic effects of the ingestion of Eupatorium buniifolium (Asteraceae) essential oil were studied on nurse honeybees using laboratory assays. Survival, food consumption, and the effect on the composition of cuticular hydrocarbons (CHC) were assessed. CHC were chosen due to their key role as pheromones involved in honeybee social recognition. While food consumption and survival were not affected by the consumption of the essential oil, CHC amounts and profiles showed dose-dependent changes. All groups of CHC (linear and branched alkanes, alkenes and alkadienes) were altered when honeybees were fed with the highest essential oil dose tested (6000 ppm). The compounds that significantly varied include n-docosane, n-tricosane, n-tetracosane, n-triacontane, n-tritriacontane, 9-tricosene, 7-pentacosene, 9-pentacosene, 9-heptacosene, tritriacontene, pentacosadiene, hentriacontadiene, tritriacontadiene and all methyl alkanes. All of them but pentacosadiene were up-regulated. On the other hand, CHC profiles were similar in healthy and Nosema -infected honeybees when diets included the essential oil at 300 and 3000 ppm. Our results show that the ingestion of an essential oil can impact CHC and that the effect is dose-dependent. Changes in CHC could affect the signaling process mediated by these pheromonal compounds. To our knowledge this is the first report of changes in honeybee cuticular hydrocarbons as a result of essential oil ingestion.

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Chronic exposure to glyphosate induces transcriptional changes in honey bee larva: A toxicogenomic study

Cited by 44 publications

References 70 publications

Sleep in honey bees is affected by the herbicide glyphosate

Sleep in honey bees is affected by the herbicide glyphosate

Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings

Sub-lethal effects of the consumption of Eupatorium buniifolium essential oil in honeybees

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