Chronic ethanol intake modifies renin–angiotensin system-regulating aminopeptidase activities in mouse cerebellum

Mayas, Marı́a Dolores; Ramírez-Expósito, María Jesús; García, María Jesús García; Carrera, Marı́a Pilar; Cobo, Manuela; Camacho, Bernardo; Martínez-Martos, José Manuel

doi:10.1016/j.npep.2004.10.003

Cited by 5 publications

(1 citation statement)

References 42 publications

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“…Chronic ethanol intake has been reported to decrease APA and APN activities in mouse cerebellum [22], supporting previous findings where the increased Ang II levels were found after ethanol intake [6, 23]. Moreover, time-dependent reduction of lysosomal APN activity in mouse kidney was demonstrated 14 days after 10 and 20% ethanol administration [24].…”

Section: Discussionsupporting

confidence: 83%

Ethanol Inhibitory Effect on Rat Kidney Brush Border Aminopeptidases

Vlahović¹,

Cvetković²,

Nikolić³

et al. 2007

Nephron Exp Nephrol

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Background/Aim: Confusing data have been reported about the effect of ethanol or its metabolic products on blood pressure. The pressor agent, angiotensin II (Ang II), is found to be susceptible to degradation by different enzymes known as angiotensinases. We have studied the effects of ethanol and L-NAME, an inhibitor of nitric oxide synthase, consumption on rat serum and kidney ectoenzymes: aminopeptidase N (APN) and aminopeptidase A (APA). Methods: Enzymatic activity of both enzymes was determined spectrophotometrically in serum and 10% homogenates of the rat kidney cortex using appropriate chromogenic substrates. Results: After 2 weeks of treatment with ethanol and L-NAME, blood urea and creatinine levels were significantly increased. The activities of APN (EC 3.4.11.2) and APA (EC 3.4.11.7) were reduced in serum as well as in kidney tissue during this period. L-NAME significantly attenuated activities of both enyzmes. Ethanol and L-NAME given simultaneously did not have an additional effect on the activity of investigated enzymes. Conclusion: Hypertension caused by chronic ethanol treatment as well as L-NAME administration could be associated with the reduction of APN and APA activity. Possible ethanol- and L-NAME-mediated inhibition of angiotensins degrading aminopeptidases could potentiate their effects on blood pressure.

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