Chronic effects of clofibric acid in zebrafish (Danio rerio): A multigenerational study

Coimbra, Ana M.; Peixoto, Maria João; Coelho, Inês; Lacerda, Ricardo; Carvalho, António Paulo; Gesto, Manuel; Lyssimachou, Angeliki; Lima, Daniela Delwing-de; Soares, José; André, Ana; Capitão, Ana; Castro, L. Filipe C.; Santos, Miguel M.

doi:10.1016/j.aquatox.2015.01.013

Cited by 50 publications

(17 citation statements)

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“…Lpl gene expression was increased, but no alteration was observed in the ppars genes (Prindiville et al, 2011). These results seem to be in line with data from mammalian models, as fibrates are known PPARα agonists and this NR is associated with fatty acid uptake and oxidation (Coimbra et al, 2015). In contrast to most studies reported in this review, fibrates mostly led to the depletion of lipid tissue levels.…”

Section: Fibratessupporting

confidence: 88%

“…In male livers of the F0, the expression of pparα and acox1 increased in the animals exposed to the highest concentration, and in F1 although the expression of pparγ increased, the expression of apoa-1 and pparβ1 decreased. This study reveals an opposite pattern of response in the descendants when compared with the parent generation (Coimbra et al, 2015). pparα expression also increased in Cyprinus carpio exposed to 20 mg/L of CA during 4 days and acox1 expression increased not only in the animals exposed to 20 mg/L but also in the animals exposed at a lower concentration (4 μg/L).…”

Section: Fibratesmentioning

confidence: 63%

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Obesogens in the aquatic environment: an evolutionary and toxicological perspective

Capitão

Lyssimachou

Castro

et al. 2017

Environment International

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The rise of obesity in humans is a major health concern of our times, affecting an increasing proportion of the population worldwide. It is now evident that this phenomenon is not only associated with the lack of exercise and a balanced diet, but also due to environmental factors, such as exposure to environmental chemicals that interfere with lipid homeostasis. These chemicals, also known as obesogens, are present in a wide range of products of our daily life, such as cosmetics, paints, plastics, food cans and pesticide-treated food, among others. A growing body of evidences indicates that their action is not limited to mammals. Obesogens also end up in the aquatic environment, potentially affecting its ecosystems. In fact, reports show that some environmental chemicals are able to alter lipid homeostasis, impacting weight, lipid profile, signaling pathways and/or protein activity, of several taxa of aquatic animals. Such perturbations may give rise to physiological disorders and disease. Although largely unexplored from a comparative perspective, the key molecular components implicated in lipid homeostasis have likely appeared early in animal evolution. Therefore, it is not surprising that the obesogen effects are found in other animal groups beyond mammals. Collectively, data indicates that suspected obesogens impact lipid metabolism across phyla that have diverged over 600 million years ago. Thus, a consistent link between environmental chemical exposure and the obesity epidemic has emerged. This review aims to summarize the available information on the effects of putative obesogens in aquatic organisms, considering the similarities and differences of lipid homeostasis pathways among metazoans, thus contributing to a better understanding of the etiology of obesity in human populations. Finally, we identify the knowledge gaps in this field and we set future research priorities.

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Section: Fibratessupporting

confidence: 88%

Section: Fibratesmentioning

confidence: 63%

Obesogens in the aquatic environment: an evolutionary and toxicological perspective

Capitão

Lyssimachou

Castro

et al. 2017

Environment International

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“…Another compound which is widely found in the aquatic environment in the ng/L range (Corcoran et al, 2015;Fent et al, 2006) is clofibric acid, the active metabolite of the antilipidemic agent clofibrate (Castillo et al, 2000;Lindberg et al, 2010). Clofibric acid is a PPAR-α receptor agonist leading to increased beta-oxidation and decreased triglyceride secretion but about its potential effect on aquatic organisms little is known (Coimbra et al, 2015;Corcoran et al, 2015;Fent et al, 2006).…”

mentioning

confidence: 99%

ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss)

Kropf

Segner

Fent

2016

Comparative Biochemistry and Physiology Part C: Toxicology &

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Embryos of oviparous fish, in contrast to (ovo) viviparous species, develop in the aquatic environment, and therefore need solute transport systems at their body surfaces for maintaining internal homeostasis and defending against potentially harmful substances. We hypothesized that solute transporters undergo changes in tissue distribution from the embryo to the larval stage. We therefore studied the mRNA profiles of eight ABC transporters (abcb1a, abcb1b, abcc1, abcc2, abcc3, abcc4, abcc5, abcg2) and three solute carriers (oatp1d, putative oatp2 putative, mate1) in different body regions (head, yolk sac epithelium, abdominal viscera, skin/muscles) of developing rainbow trout. Additionally, we investigated mRNA levels of phase I (cyp1a, cyp3a) and phase II (gstp, putative ugt1, putative ugt2) biotransformation enzymes. The study covered the developmental period from the eleuthero-embryo stage to the first-feeding larval stage (1-20days post-hatch, dph). At 1dph, transcripts of abcc2, abcc4, abcg2, cyp3a, gstp, putative mate1, and putative oatp2 occurred primarily in the yolk sac epithelium, whereas at later stages expression of these genes was predominantly observed in the abdominal viscera. The functional activity of ABC transporters in fish early life stages was assessed by rhodamine B accumulation assays. Finally, we investigated the potential impact of xenobiotics (clotrimazole, clofibric acid) on the ABC and biotransformation systems of trout early life stages. While clofibric acid had no effect, clotrimazole lead to an increased rhodamine B accumulation. The results provide evidence that the transition from the eleuthero-embryo to the larval stage is accompanied by a major alteration in tissue expression of ABC transporters.

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“…It affects muscle through changing its composition. Chronic, lifetime exposure to CA induces in the F1 generation decreased growth and lower muscle triglyceride content and affects male gonad development [71]. …”

Section: Drugs Stimulants/depressants and Cosmeticsmentioning

confidence: 99%

Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function

Dubińska–Magiera

Daczewska

Lewicka

et al. 2016

IJMS

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The rapid progress in medicine, agriculture, and allied sciences has enabled the development of a large amount of potentially useful bioactive compounds, such as drugs and pesticides. However, there is another side of this phenomenon, which includes side effects and environmental pollution. To avoid or minimize the uncontrollable consequences of using the newly developed compounds, researchers seek a quick and effective means of their evaluation. In achieving this goal, the zebrafish (Danio rerio) has proven to be a highly useful tool, mostly because of its fast growth and development, as well as the ability to absorb the molecules diluted in water through its skin and gills. In this review, we focus on the reports concerning the application of zebrafish as a model for assessing the impact of toxicants on skeletal muscles, which share many structural and functional similarities among vertebrates, including zebrafish and humans.

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Chronic effects of clofibric acid in zebrafish (Danio rerio): A multigenerational study

Cited by 50 publications

References 50 publications

Obesogens in the aquatic environment: an evolutionary and toxicological perspective

Obesogens in the aquatic environment: an evolutionary and toxicological perspective

ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss)

Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function

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