Chromosomes of gaur cross domestic cattle hybrids

Bongso, T. A.; Hilmi, M.; Sopian, Muhammad Ali; Zulkifli, Syaizwan Zahmir

doi:10.1016/s0034-5288(18)30850-6

Cited by 11 publications

(7 citation statements)

References 9 publications

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“…A comparison between the GTG-banded karyotype of Thai gaur with a domestic cattle standard showed the 2 pairs of submetacentric autosomes of Thai gaur occurred from the centric fusion of 1;29 and 2;28 acrocentric autosomes, respectively. Moreover, this is also consistent with Bongso et al (1988), who reported the same diploid number (2nϭ56) in Malayan gaur as in Thai gaur. The Malayan gaur Oryx spp., and Antilope spp.…”

Section: Resultssupporting

confidence: 90%

Robertsonian Translocation [56,XX and 57,XY,rob(1;29)] in Captive Thai Gaur (Bos gaurus readei) by Conventional, GTG-Banding, CBG-Banding and Ag-NOR Banding Techniques

et al. 2011

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SummaryRobertsonian translocation, or centric fusion, which is known to be the most common mechanism in karyotype evolution of bovid species, was observed and described in captive Thai gaur (Bos gaurus readei). Blood samples were taken from 2 male and 2 female captive Thai gaur. After the standard whole blood lymphocyte culture in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional staining, GTG-banding, CBGbanding and Ag-NOR banding techniques were applied to stain the chromosomes. The number of diploid chromosomes (2n) were 57 in 2 males and 56 in 2 females instead of the normal 2nϭ58. The fundamental number (NF) was 62 in both the 2 males and 2 females. The autosome consists of 3 submetacentric and 52 telocentric chromosomes in males, and 4 submetacentric and 50 telocentric chromosomes in females. The X chromosome was a large submetacentric chromosome and the Y chromosome was the smallest metacentric chromosome. Eight telocentric chromosomes and 2 submetacentric chromosomes (5 autosome pairs) were identified as satellite chromosomes which showed clearly observable nucleolar organizer regions (NORs). The presence of an extra submetacentric chromosome and the loss of 2 telocentric chromosomes [57,XY,rob(1;29)] were observed in both males. Also, the presence of 2 extra submetacentric chromosomes and the loss of 4 telocentric chromosomes [56,XX,rob(1;29)] were observed in both females. Results from the GTGbanding and CBG-banding techniques analyses confirmed that the 2 autosomes of male (2nϭ57) and 4 autosomes of female (2nϭ56) involved in the translocation are the chromosome pairs 1 and 29, which are common in translocation of the bovid species.

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Section: Resultssupporting

confidence: 90%

Robertsonian Translocation [56,XX and 57,XY,rob(1;29)] in Captive Thai Gaur (Bos gaurus readei) by Conventional, GTG-Banding, CBG-Banding and Ag-NOR Banding Techniques

et al. 2011

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“…1a), whereas the gaur is unique with a chromosome complement of 58 ( Fig. 1b; Riggs et al, 1997;Bongso and Hilmi, 1988). Cytogenetic analysis on the cloned cell strains revealed a normal karyotype with a modal chromosome number of 58 (Fig.…”

Section: Resultsmentioning

confidence: 97%

Cloning of an Endangered Species (Bos gaurus) Using Interspecies Nuclear Transfer

Lanza

Cibelli

Díaz

et al. 2000

Cloning

327

205

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Approximately 100 species become extinct a day. Despite increasing interest in using cloning to rescue endangered species, successful interspecies nuclear transfer has not been previously described, and only a few reports of in vitro embryo formation exist. Here we show that interspecies nuclear transfer can be used to clone an endangered species with normal karyotypic and phenotypic development through implantation and the late stages of fetal growth. Somatic cells from a gaur bull (Bos gaurus), a large wild ox on the verge of extinction, (Species Survival Plan Ͻ 100 animals) were electrofused with enucleated oocytes from domestic cows. Twelve percent of the reconstructed oocytes developed to the blastocyst stage, and 18% of these embryos developed to the fetal stage when transferred to surrogate mothers. Three of the fetuses were electively removed at days 46 to 54 of gestation, and two continued gestation longer than 180 (ongoing) and 200 days, respectively. Microsatellite marker and cytogenetic analyses confirmed that the nuclear genome of the cloned animals was gaurus in origin. The gaur nuclei were shown to direct normal fetal development, with differentiation into complex tissue and organs, even though the mitochondrial DNA (mtDNA) within all the tissue types evaluated was derived exclusively from the recipient bovine oocytes. These results suggest that somatic cell cloning methods could be used to restore endangered, or even extinct, species and populations. 79

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“…In Africa, introgression of Indian zebu bulls (Bos indicus) in taurine herds occurs and has improved the tolerance of the cattle (Bos taurus) to hot and dry environments (Epstein, 1971;Bradley et al, 1994Bradley et al, , 1996Loftus et al, 1994b;Frisch et al, 1997;Hanotte et al, 2000). Bongso et al (1988) described a spontaneous hybridization of wild gaur and zebu.…”

Section: Introductionmentioning

confidence: 99%

Hybridization of banteng (Bos javanicus) and zebu (Bos indicus) revealed by mitochondrial DNA, satellite DNA, AFLP and microsatellites

et al. 2003

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Hybridization between wild and domestic bovine species occurs worldwide either spontaneously or by organized crossing. We have analysed hybridization of banteng (Bos javanicus) and zebu (Bos indicus) in south-east Asian cattle using mitochondrial DNA (PCR-RFLP and sequencing), AFLP, satellite fragment length polymorphisms (SFLP or PCR-RFLP of satellite DNA) and microsatellite genotyping. The Indonesian Madura zebu breed is reputed to be of hybrid zebu-banteng origin, but this has never been documented and Bali cattle are considered to be a domesticated form of banteng. The banteng mitochondrial type was found in all animals sampled on the isle of Bali, Indonesia, but only in 35% of the animals from a Malaysian Bali-cattle population.The Madura animals also carried mitochondrial DNA of either zebu and banteng origin. In both populations, zebu introgression was confirmed by AFLP and SFLP. Microsatellite analysis of the Malaysian Bali population revealed for 12 out of 15 loci screened, Bali-cattle-specific alleles, several of which were also found in wild banteng animals. The tools we have described are suitable for the detection of species in introgression studies, which are essential for the genetic description of local breeds and the preservation of their economic and cultural value.

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Chromosomes of gaur cross domestic cattle hybrids

Cited by 11 publications

References 9 publications

Robertsonian Translocation [56,XX and 57,XY,rob(1;29)] in Captive Thai Gaur (Bos gaurus readei) by Conventional, GTG-Banding, CBG-Banding and Ag-NOR Banding Techniques

Robertsonian Translocation [56,XX and 57,XY,rob(1;29)] in Captive Thai Gaur (Bos gaurus readei) by Conventional, GTG-Banding, CBG-Banding and Ag-NOR Banding Techniques

Cloning of an Endangered Species (Bos gaurus) Using Interspecies Nuclear Transfer

Hybridization of banteng (Bos javanicus) and zebu (Bos indicus) revealed by mitochondrial DNA, satellite DNA, AFLP and microsatellites

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