Chromosome studies in the genus Cucumis

Dane, Fenny; Tsuchiya, T.

doi:10.1007/bf00041569

Cited by 41 publications

(28 citation statements)

References 7 publications

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“…hardwickii using isozymes, restriction fragment length polymorphisms (RFLPs), and random amplified polymorphic DNAs (RAPDs) indicated that diversity in C. sativus var. sativus is relatively low (3-8%) when compared with other allogamous Cucumis species (10-25%) (Dane 1976(Dane , 1983EsquinasAlcazar 1977;Knerr et al 1989;Dijkhuizen et al 1996;Horejsi and Staub 1999). Levels of allelic polymorphism in C. sativus var.…”

Section: Cucumis Sativusmentioning

confidence: 97%

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Lebeda¹,

Widrlechner²,

Staub³

et al. 2006

Genetic Resources Chromosome Engineering &Amp; Crop Improvement

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Section: Cucumis Sativusmentioning

confidence: 97%

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Lebeda¹,

Widrlechner²,

Staub³

et al. 2006

Genetic Resources Chromosome Engineering &Amp; Crop Improvement

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“…Ouchterlony double diffusion was used to determine if the ETHYLENE INDUCTION AND DISTRIBUTION OF PEROXIDASE Ouchterlony double diffusion assay for antigenicity of pi 6 peroxidase to rabbit antibodies raised against pl 9 and pl 4 peroxidase. Clockwise from left to right, the outer wells contain either purified pl 9 or pl 4 peroxidase, or the third column fraction from the Sephadex Mono Q column containing the pi 6 peroxidases.…”

Section: Isoelectric Focusingmentioning

confidence: 99%

“…We observed that while ethylene also induced peroxidases with pIs of 4, 6, and 9 from Cucurbita radicans, these proteins did not react with cucumber antibodies in the-RID assay. IEF data (not shown) from other (6). According to Jeffrey (10) (1 3).…”

Section: Isoelectric Focusingmentioning

confidence: 99%

Hormonal Regulation and Distribution of Peroxidase Isoenzymes in the Cucurbitaceae

Abeles

Biles²,

Dunn³

1989

Plant Physiol.

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Ethylene enhanced the levels of peroxidases in the roots, stems, leaves, and cotyledons of 2-week-old cucumber Cucumis sativus cv Poinsett 76 seedlings. Antibodies to the isoelectric point (pi) 9 and pi 4 isoenzymes were used in a radial immunodiffusion assay to demonstrate that ethylene induced similar peroxidases in other cultivars of C. sativus, other species of Cucumis and other genera of Cucurbitaceae. Examination of ethylene-induced peroxidases, using isoelectric focusing gels, demonstrated the presence of a series of other peroxidases, mostly slightly acidic, whose isoelectric focusing pH was approximately 6. These pi 6 peroxidases were partially purified on a cation exchange column. Ouchterlony double diffusion gels indicated that these proteins cross-reacted with antibodies to both the pi 9 and pi 4 peroxidase. The data presented here suggest that the induction of peroxidase isoenzymes during ethyleneinduced senescence is a common response in this family of plants. In addition, antibody and isoelectric focusing studies indicate that both acidic and basic peroxidase are highly conserved in members of this family.

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“…The most standard references demonstrated that Cucumis sativus had the chromosome number of 2n=14, which was different from that of the other species of Cucumis (Dane and Tsuchiya 1976;Dane et al 1980).The basic karyotype was analyzed by the conventional method (Trivedi and Roy 1970;Sing and Roy 1974;Dane and Tsuchiya 1976;Ramachandran and Seshadri 1986;Hoshi et al 1998) and differential staining method such as C-banding method (Ramachandran and Seshadri 1986;Chen et al 1998;Hoshi et al 1998;Koo et al 2002), chromomycin A 3 (CMA) method and 4',6-diamidino-2-phenylindole (DAPI) staining method Hoshi et al 1999;Hoshi et al 2008). According to the previous studies, it was demonstrated that the chromosomes of respective cucumber were possible to distinguish by using banding pattern.…”

mentioning

confidence: 92%

Cytogenetic comparison among three cultivars of cucumber (Cucumis sativus L.) by using post-heated DAPI band, 45S and 5S rDNA sites

et al. 2009

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ABSTRACT. The positions of heterochromatic bands, 45S and 5S rDNA regions were detected on the chromosomes in three cultivars of cucumber (Cucumis sativus L.). The post-heated DAPI bands, which revealed heterochromatic region, were observed on the both terminal and pericentromeric regions of most of the chromosomes in three cultivars. However, these band patterns were different between European and Eastern Asian cultivars. The major 45S rDNA sites were found around the centoromeric region of the chromosomes 1, 2 and 5, and the minor 45S rDNA sites were found around centoromeric region of the chromosomes 3 and 7. The positional variation of this region was not found among three cultivars, but size difference was observed in the chromosome 5. The 5S rDNA site was observed on the interstitial region of the chromosome 6 in all of the cultivars studied. These results indicated that the variation of chromosome structure among cucumber cultivars occurred in the heterochromoatic region and suggested that the genetic variation in cucumber cultivars would be revealed by strict analysis and comparison for the position and size of heterochromatic region.

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Chromosome studies in the genus Cucumis

Cited by 41 publications

References 7 publications

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Hormonal Regulation and Distribution of Peroxidase Isoenzymes in the Cucurbitaceae

Cytogenetic comparison among three cultivars of cucumber (Cucumis sativus L.) by using post-heated DAPI band, 45S and 5S rDNA sites

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