Hormonal Regulation and Distribution of Peroxidase Isoenzymes in the Cucurbitaceae

Abeles, Frederick B.; Biles, Charles L.; Dunn, Linda J.

doi:10.1104/pp.91.4.1609

Cited by 22 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with earlier work showing that ethylene increased the levels of pI 4 and pI 9 peroxidase in cotyledonary tissue ( 1). Plants have a number of peroxidase isozymes.…”

Section: Discussionsupporting

confidence: 93%

Xylem Sap Proteins

Biles¹,

Abeles²

1991

Plant Physiol.

Self Cite

117

View full text Add to dashboard Cite

Xylem sap from apple (Malus domestica Borkh), peach (Prunus persica Batsch), and pear (Pyrus communis L.) twigs was collected by means of pressure extrusion. This sap contained a number of acidic peroxidases and other proteins. Two other sources of xylem sap used in this study were stem exudates and guttation fluid. Similar peroxidases were also found in stem exudates and guttation fluids of strawberry (Fragaria x ananassa Duch.), tomato (Lycopersicum esculentum L.), and cucumber (Cucumis sativus L.). lsoelectnc focusing activity gels showed that two peroxidases (isoelectric point [pi] Peroxidase isozymes can be used to test the idea that xylem sap contains a unique set of proteins. The presence of a peroxidase isozyme in xylem sap which is not found in the surrounding leaftissue can be used as evidence that specialized proteins are secreted into the vascular system. Since peroxidases catalyze polymerization reactions, experiments were conducted to test the idea that xylem sap peroxidase plays a role in wound plugging. MATERIALS AND METHODS Plant MaterialTerminal stems of apple (Malus domestica Borkh), peach (Prunus persica Batsch), and pear (Pyrus communis L.) were collected from the field. Sections of 20 cm by approximately 1 cm were excised from 1-year-old wood. The bark from a 1-cm section of the lower end of the stem was ringed and removed. A plastic hose attached to a syringe was placed over the exposed xylem tissue. Plastic hose clamps were used to minimize leakage. Xylem sap was expressed by forcing a solution of bromophenol blue (A600 = 0.3) through the tissue. This dye has a negative charge at pH 6. Positively charged dyes such as methyl green adhered to the negatively charged xylem. About I mL of exudate was collected by applying pressure to the syringe. Dilution ofxylem sap by bromophenol blue forcing solution was calculated by measuring the optical density of the solution collected at 600 nin. Using this technique the respective amount of forcing solution in peach, pear, and apple exudate was 90, 32, and 34%. The SE of these measurements was 5.3%. The difference in xylem sap dilution with forcing solution may be due to the difference in the size of xylem elements. Peach xylem has a larger range of cell diameters than apple and pear. This lack of uniformity would cause most of the forcing solution to bypass smaller cells because large cells are the path of least resistance. Xylem sap was filtered through a 0.22 ,um nylon filter prior to concentration on an Amicon Centricon-30 microconcentrator (W. R. Grace, Danvers, MA).Guttation fluid or stem exudates were collected by means of a handheld pipetter. Guttation fluid was collected from field-grown strawberry (Fragaria x ananassa Duch.) plants. Guttation fluid was also collected from greenhouse grown tomato plants (Lycopersicum esculentum L.). Guttation fluid from tomato was collected after the plants were stored in 100% relative humidity chambers for 18 h. Care was taken

show abstract

“…This is consistent with earlier work showing that ethylene increased the levels of pI 4 and pI 9 peroxidase in cotyledonary tissue ( 1). Plants have a number of peroxidase isozymes.…”

Section: Discussionsupporting

confidence: 93%

Xylem Sap Proteins

Biles¹,

Abeles²

1991

Plant Physiol.

Self Cite

117

View full text Add to dashboard Cite

show abstract

“…Now it can be easily detected that the case of stress POD is very similar to that of stress ethylene. More interestingly, there are publications reporting that both ethylene and POD can stimulate each other (Abeles et al, 1989;Gasper et al, 1985;Osswald et al, 1989).…”

Section: Soluble Pod Activity (%) 120mentioning

confidence: 99%

Ethylene production in rice bronzing leaves induced by ferrous iron

Peng

Yamauchi

1993

Plant Soil

View full text Add to dashboard Cite

Bronzing, a nutritional disorder of rice plants which is widely distributed in tropical lowlands, was induced by dipping the cut end of rice leaves into FeSO 4 solution (pH 3.5). Ethylene production; the activities of peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase; and the effects of Co 2*, aminoethoxyvinylglycine, Ag +, cycloheximide, and 1-aminocyclopropane-l-carboxylate, were investigated in the course of bronzing development. It was found that ethylene production could be stimulated up to about 20 times that of the control by Fe 2÷, and a peak could be reached at about 24 h after incubation. The Fe2+-treated leaves also had 10-fold higher peroxidase activity than the control, whereas in vitro enzyme activity was inhibited by Fe 2*. Cycloheximide retarded in vivo stimulation of peroxidase, indicating that in vivo stimulation resulted from inducing de novo synthesis of the enzyme. No changes in the activities of phenylalanine ammonia-lyase and polyphenol oxidase were observed. The results, obtained from the incubation of leaves with Co 2÷, aminoethoxyvinylglycine, Ag+, cycloheximide, or 1-aminocyclopropane-l-carboxylate, showed that ethylene production was the effect of Fe 2+ stress and that it was not involved in the process of bronzing development, which is probably an acclimation process to enable plants to cope with stress. The accelerated peroxidase activity may be associated with bronzing development. Abbreviations: ACC -1-aminocyclopropane-l-carboxylic acid, AVG -aminoethoxyvinylglycine, EFEethylene forming enzyme, P A L -phenylalanine ammonia-lyase, P O D -peroxidase, P P O -polyphenol oxidase, S E -standard error

show abstract

“…These phenomena are often accompanied by an increase POD activity (Kim et al 2000;De and De 2003). Ethylene has been shown to increase POD activity in sweet potato, cucumber, pea, tomato, and tobacco and also to affect mRNA expression of POD in suspension cultured cells of peanut (Abeles et al 1989;Kim et al 2000). Kar and Mishra (1976) have indicated that in rice leaves, POD and PPO activity were increased with the progress of senescence while CAT activity decreased.…”

Section: Resultsmentioning

confidence: 96%

“…Detoxification of H 2 O 2 is mediated by catalase localized in peroxisomes (Weckx and Clisters 1997;Nandwal et al 2000). It has been reported that ethylene (100 ll/l) enhanced the activity of acidic, neutral and basic peroxidases in Cucumis sativus seedlings (Abeles et al 1989). Stimulation of ethylene synthesis has been reported resulting in the generation of reactive oxygen species (Wang et al 2002).…”

Section: Introductionmentioning

confidence: 98%

In vivo and in vitro effects of ethephon on some oxidative enzymes in spinach leaves

2007

View full text Add to dashboard Cite

In vivo and in vitro effects of ethephon, an ethylene-generating compound, on some oxidative enzymes; polyphenol oxidase (PPO, 1.10.3.1), peroxidase (POD, 1.11.1.7) and catalase (CAT, 1.11.1.6) in spinach leaves were investigated. The plants were grown under controlled conditions in sand culture in 60 days. Ethephon was applied as two doses at 0.1 and 1 mM. The leaves were harvested in the first and fifth days after ethephon treatment. In order to determine the effect of ethephon on the enzymes in vitro, the enzymes from leaves in control group were partially purified. In vivo, PPO activities were increased significantly at both concentrations whereas significant enhancement of POD activity was determined only at 1 mM. CAT activity was significantly reduced by ethephon through time. In vitro, POD and CAT activities increased slightly at 0.033 and 0.33 mM ethephon concentrations, as PPO activity did not increase at the same concentrations. But these activities of enzymes decreased significantly at 33.3 mM of ethephon concentration for POD and PPO and 26.6 mM for CAT. As a result, the increase of activity for PPO and POD or decrease of activity for CAT in vivo may be caused by not direct inhibition or activation of enzymes but a mechanism at transcriptional level induced by ethylene.

show abstract

Hormonal Regulation and Distribution of Peroxidase Isoenzymes in the Cucurbitaceae

Cited by 22 publications

References 17 publications

Xylem Sap Proteins

Xylem Sap Proteins

Ethylene production in rice bronzing leaves induced by ferrous iron

In vivo and in vitro effects of ethephon on some oxidative enzymes in spinach leaves

Contact Info

Product

Resources

About