This investigation confirms the existence of a high degree of incompatibility between species of Trifolium . Two techniques were developed in an attempt to overcome the barriers to hybridization . The first deals with the use of grafting as a screening technique for compatible genotypes, and the second is an embryo culture method for salvaging hybrid embryos which would otherwise abort . The value of these methods is discussed, and the results are compared with those from hybridizations of non-grafted material and of seed set without artificial embryo culture . Hybrids were obtained from three species combinations at different levels of ploidy . SPECIES HYBRIDIZATION IN TRIFOLIUM 1 6 5