Chromosome number and ploidy level of some South American species of Schizachyrium (Poaceae, Andropogoneae)

Peichoto, Myriam Carolina; Galdeano, Florencia; Arbo, María Mercedes; Neffa, Viviana G. Solís

doi:10.1007/s00606-010-0403-1

Cited by 7 publications

(12 citation statements)

References 35 publications

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“…One group of S. sanguineum plastomes clustered with American Schizachyrium (S. scoparium and S. spicatum) and the other with Asian species (S. exile and S. brevifolium). All S. sanguineum samples were collected in Thailand, suggesting either a deep coalescence of chloroplast haplotypes within S. sanguineum that predate the geographical disjunction of other Schizachyrium species or admixture of haplotypes through allopolyploidy as discussed below (Peichoto et al 2011;Estep et al 2014).…”

Section: Systematic Botanymentioning

confidence: 99%

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Phylogenomics of Andropogoneae (Panicoideae: Poaceae) of Mainland Southeast Asia

et al. 2017

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Section: Systematic Botanymentioning

confidence: 99%

“…This species has been reported to have a great range of allopolyploid levels based on cytology and analysis of low-copy loci (Peichoto et al 2011;Estep et al 2014). The two independent S. sanguineum clades could represent distinct allopolyploidy events.…”

Section: Systematic Botanymentioning

confidence: 99%

Phylogenomics of Andropogoneae (Panicoideae: Poaceae) of Mainland Southeast Asia

et al. 2017

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“…Based on the scores of the four pollen grain parameters, we performed separate non-metric multidimensional scalings (NMDSs) both for the C3/C4 group and diploid/polyploid groups [using the meta-multidimensional scaling (MDS) function available in the vegan package of R; Oksanen et al 2013]. The NMDS scores of the four pollen grain parameters were plotted for C3/C4 species and diploid/polyploid species separately.…”

Section: Non-metric Multidimensional Scaling (Nmds)mentioning

confidence: 99%

Trends of pollen grain size variation in C3 and C4 Poaceae species using pollen morphology for future assessment of grassland ecosystem dynamics

Jan

Schüler

Behling

2014

Grana

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This pilot study aims to find whether C3 and C4 Poaceae species can be separated by their pollen grain size. One hundred and sixty Poaceae species were prepared for pollen grain size analyses using standard methods. The whole dataset included 70 species from northern Pakistan, 35 species from Tanzania, 25 from South America and 30 from Germany. We measured four pollen grain parameters, i.e. pollen grain length, pollen grain width, pore diameter and annulus width. Results of the statistical tests and multivariate analyses performed on our specific dataset confirmed that C3 polyploid and C4 polyploid species show trends of larger grain size than the respective diploid species. Results of our fresh Poaceae grains should be applied on fossil pollen embedded in environmental archives for reconstructing past climate, for detecting shifts between their C3/C4 Poaceae compositions and assessing the dynamics of grassland ecosystems.

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“…Ploidy variation within Schizachyrium has been seen from 2n = 2x = 20 to 2n = 10x = 100 (Peichoto et al, 2011). Schizachyrium scoparium has been found to be 2n = 4x = 40 (Carman and Hatch, 1982); S. condensatum has been found to be 2n = 2x = 20 and 2n = 4x = 40 (Peichoto et al, 2011); and S. sanguineum has been found to be 2n = 2x = 20, 2n = 3x = 30, 2n = 4x = 40, 2n = 5x = 50, 2n = 6x = 60, 2n = 7x = 70, 2n = 8x = 80, and 2n = 10x = 100 (Peichoto et al, 2011). Ploidy variation was determined by flow cytometry on the 75 S. scoparium accessions obtained from the USDA and all plants had a ploidy level the same as the assumed tetraploid (2n = 4x = 40).…”

Section: Methodsmentioning

confidence: 99%

Development and Characterization of Microsatellite Markers for a Little Bluestem Collection

Harris‐Shultz

Harrison²,

Wadl

et al. 2015

J. Amer. Soc. Hort. Sci.

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Little bluestem (Schizachyrium scoparium) is a perennial bunchgrass that is native to North American prairies and woodlands from southern Canada to northern Mexico. Originally used as a forage grass, little bluestem is now listed as a major U.S. native, ornamental grass. With the widespread planting of only a few cultivars, we aimed to assess the ploidy level and genetic diversity among some popular cultivars and accessions in the U.S. Department of Agriculture National Plant Germplasm System collection. Ten microsatellite markers, with successful amplification, were developed by using sequences available in Genbank and additional simple sequence repeat (SSR) markers were generated by using ion torrent sequencing of a genomic library created from the cultivar The Blues. A total of 2812 primer sets was designed from high-throughput sequencing, 100 primer pairs were selected, and 82 of these primers successfully amplified DNA from the Schizachyrium accessions. Only 35 primer pairs, generating 102 scored fragments, were polymorphic among S. scoparium accessions. Twenty-two primer pairs generated more than four fragments per accession. The use of a repetitive sequence identifier found that of 117 examined sequences, only nine sequences did not have similarity to DNA transposons, retrotransposons, viruses, or satellite sequences. The most frequently identified fragments were the long terminal repeat retrotransposons Gypsy (177 fragments) and Copia (98 fragments) and the DNA transposon EnSpm (60 fragments). Using the software program Structure, cluster analysis of the SSR data for S. scoparium revealed four groups. The lowest genetic similarity between little bluestem samples was 86%, which was surprising as a high degree of morphological variation is seen in this species. Furthermore, no variation in ploidy level was seen among little bluestem samples. These microsatellite markers are the first sequence-specific markers designed for little bluestem and can serve as a resource for future genetic studies.

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Chromosome number and ploidy level of some South American species of Schizachyrium (Poaceae, Andropogoneae)

Cited by 7 publications

References 35 publications

Phylogenomics of Andropogoneae (Panicoideae: Poaceae) of Mainland Southeast Asia

Phylogenomics of Andropogoneae (Panicoideae: Poaceae) of Mainland Southeast Asia

Trends of pollen grain size variation in C3 and C4 Poaceae species using pollen morphology for future assessment of grassland ecosystem dynamics

Development and Characterization of Microsatellite Markers for a Little Bluestem Collection

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