Chromosome number and isoenzyme variation in Kentucky bluegrass cultivars and plants regenerated from tissue culture.

Wu, Lin; Jampates, Rachanee

doi:10.1508/cytologia.51.125

Cited by 23 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, a typical seed lot of the cultivar 'Baron' has been shown to contain 70 to 90% apomictically-derived seeds and 10 to 30% sexuallyderived seeds (Wu & Jampates, 1986). Kentucky bluegrass has a type of gametophytic apomixis called apospory, in which a sporophytic cell near the original embryo sac begins to divide to form a new embryo sac from a 2n nucleus (Akerberg, 1943;Muntzing, 1933;Tinney, 1940).…”

Section: Introductionmentioning

confidence: 99%

Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin

et al. 2006

View full text Add to dashboard Cite

Plants were regenerated from tissue cultures of embryos dissected from seeds that were harvested from a selfpollinated clonal selection of Kentucky bluegrass (Poa pratensis L.) 'Baron', an apomictic cultivar. Plants were regenerated from 35 embryo-derived callus cultures of the 3280 embryos that were plated. Flow-cytometric (FCM) and RAPD-marker analyses were performed to determine if regenerants were or were not apomictic in origin. Fifteen regenerants with a 3c DNA content were classified as arising from 2n + n aberrant embryos, which was a higher frequency than expected, based on a chi-square analysis. Of the remaining 20 regenerants with a 2c DNA content, a chi-square test showed that all could have arisen from n + n sexually-derived embryos, based on the observed segregation of n + n regenerants, which fit the expected 3:1 ratio of dominant:recessive RAPD-marker phenotypes. The apparent lack of regenerants of apomictic origin, and implications for genetic transformation and breeding of Kentucky bluegrass are discussed.Abbreviations: n + 0: haploid seedling, 2n + 0: apomictically-derived seedling, n + n: sexually-derived seedling, 2n + n: aberrant seedling derived from unreduced egg and reduced sperm, FCM: Flow cytometry, RAPD: Randomly amplified polymorphic DNA, 1c: haploid, 2c: diploid, 3c: triploid

show abstract

Section: Introductionmentioning

confidence: 99%

Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Over the past 20 years many culture systems for Kentucky bluegrass were reported using various tissues as explants, i.e. mature and immature zygotic embryos (Manton et al, 1982;McDonnell and Conger, 1984;Boyd and Dale, 1986), immature inflorescences ( Van der Valk et al, 1989), shoot tips (Wu and Jampates, 1986), whole mature seeds (Krans, 1981; Van der Valk et al, 1989Van Ark et al, 1991;Griffin and Dibble, 1995;Hodges et al, 1999), and coleoptile, leaf, and stem sections of seedlings . Suspension cultures of Kentucky bluegrass were also described .…”

Section: Introductionmentioning

confidence: 99%

Stable transformation of a recalcitrant kentucky bluegrass (Poa pratensis L.) cultivar using mature seed-derived highly regenerative tissues

Lemaux

Cho

2001

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

An efficient method to produce highly regenerative tissues from seeds of a previously recalcitrant cultivar of Kentucky bluegrass (Poa pratensis L. cv. Kenblue) was established under dim-light conditions (10±30 mE m 22 s 21 , 16-h light) using media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D; 4.5 or 9.0 mM), 6-benzylaminopurine (BA; 0.44 or 2.2 mM), and a high level of cupric sulfate (5.0 mM). The tissues were co-transformed with three plasmids containing the genes for hygromycin phosphotransferase (hpt), b-glucuronidase (uidA; gus), and a synthetic green fluorescent protein gene [sgfp(S65T)]. From 463 individual explants bombarded, 10 independent transgenic events (2.2%) were obtained after a 3±4-month selection period for hygromycin resistance using 30±100 mg l 21 hygromycin B; of the 10 independent events, seven (70%) were regenerable. Stable integration of the transgene(s) in transgenic plants was confirmed by polymerase chain reaction and DNA blot hybridization analyses. Coexpression frequency of all three genes was 20%; for two transgenes, either hpt/uidA or hpt/sgfp(S65T), coexpression frequency was 30±40%.

show abstract

“…Complete apomixis was reported in only one instance, for the cultivar Spaths by Pommer (1972), who also reported the lowest value for the cultivar Steinacher (44%) . The introduction of more discriminative techniques, such as isozyme progeny testing, did not produce consistent decrements in the estimated values of apomixis in cultivars (Wu & Jampates, 1986 ;den Nijs, 1990 ;Mazzucato, 1995a).…”

Section: Resultsmentioning

confidence: 88%

Evolution and adaptedness in a facultatively apomictic grass, Poa pratensis L.

Mazzucato¹,

Falcinelli²,

Veronesi³

1996

Euphytica

View full text Add to dashboard Cite

Facultative apomixis confers great adaptedness and evolutive potential on biotypes belonging to the Poa pratensis L. agamic complex . Pioneering and recent research have dealt with and sometimes elucidated different aspects of apomictic expression, such as cytological mechanisms, distribution patterns, genetic control and environmental effects, in this species . We carried out an investigation on the mode of reproduction expressed in Italian natural populations, cultivars and selected genotypes together with an extensive time (56 years) and space (six countries) review in order to obtain a comprehensive picture of apomixis expression in P. pratensis . Histograms of the estimations were prepared . They showed that, due to breeder and natural selection, both cultivars and natural populations very frequently expressed a high degree of apomixis . Variations in the degree of apomixis across generations, different pollen donors, environments and estimation levels were generally low . It would, therefore, seem that the tenet which holds that apomixis in P. pratensis is strongly influenced by external factors should be reconsidered . Despite the fact that little is known of the genetic, physiological and molecular control underlying apomixis, the overall picture that emerges from our study helps to explain how the balance between sexual and asexual reproduction confers extreme versatility, adaptedness and evolutive capacity on this remarkable grass .

show abstract

Chromosome number and isoenzyme variation in Kentucky bluegrass cultivars and plants regenerated from tissue culture.

Cited by 23 publications

References 18 publications

Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin

Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin

Stable transformation of a recalcitrant kentucky bluegrass (Poa pratensis L.) cultivar using mature seed-derived highly regenerative tissues

Evolution and adaptedness in a facultatively apomictic grass, Poa pratensis L.

Contact Info

Product

Resources

About