Stable transformation of a recalcitrant kentucky bluegrass (Poa pratensis L.) cultivar using mature seed-derived highly regenerative tissues

Ha, Chi; Lemaux, Peggy G.; Cho, Myeong‐Je

doi:10.1007/s11627-001-0002-5

Cited by 32 publications

(13 citation statements)

References 31 publications

(25 reference statements)

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“…Among reports of successful transformation of callus, the explants from which the callus was first derived include (1) immature tassels, immature ears, or anthers in maize (Cheng et al, 2004); (2) leaf bases from maize (Sidorov et al, 2006;Ahmadabadi et al, 2007); and (3) scutellum from mature seeds in rice (Chen et al, 1998;Dai et al, 2001). Alternatively, explants used to initiate proliferating meristem cultures for subsequent transformation have included maize apical or nodal meristems (Zhong et al, 1996;Zhang et al, 2002) or mature seeds in species such as rice (Cho et al, 2004), oat (Avena sativa; Cho et al, 1999), orchardgrass (Dactylis glomerata; Cho et al, 2000a), Kentucky bluegrass (Poa pratensis; Ha et al, 2000), and fescue (Festuca sp; Cho et al, 2000b). Regardless of the culture type, all of these reports have relied on the manipulation of exogenous hormones in the culture media to produce either embryogenic callus or multiple meristems for use as the transformation target.…”

Section: Introductionmentioning

confidence: 99%

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

et al. 2016

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While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. Here, we report a transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines. For example, the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium tumefaciens transformation. However, when Bbm and Wus2 were expressed, transgenic calli were recovered from over 40% of the starting explants, with most producing healthy, fertile plants. Another limitation for many monocots is the intensive labor and greenhouse space required to supply immature embryos for transformation. This problem could be alleviated using alternative target tissues that could be supplied consistently with automated preparation. As a major step toward this objective, we transformed Bbm and Wus2 directly into either embryo slices from mature seed or leaf segments from seedlings in a variety of Pioneer inbred lines, routinely recovering healthy, fertile T0 plants. Finally, we demonstrated that the maize Bbm and Wus2 genes stimulate transformation in sorghum (Sorghum bicolor) immature embryos, sugarcane (Saccharum officinarum) callus, and indica rice (Oryza sativa ssp indica) callus.

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Section: Introductionmentioning

confidence: 99%

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

et al. 2016

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“…Various re-engineered gfp genes have recently been used as vital reporters for the transformation of many transgenic crop species (Pang et al 1996;Haseloff et al 1997;Leffel et al 1997;Vain et al 1998;van der Geest and Petolino 1998;Ahlandsberg et al 1999; 2000, 2002Jordan 2000;Kaeppler et al 2000;Ha et al 2001). In addition, gfp has recently been used as a visual selection marker for oat transformation (Kaeppler et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…The sgfp(S65T) sequence was initially tested in protein targeting studies in Arabidopsis and onion cells and is superior to uidA [β-glucuronidase (gus) gene] in terms of its nondestructiveness during assay (Chiu et al 1996). Recently it has been used for the stable transformation of wheat (Jordan 2000), barley (Cho et al 2002), oat (Kaeppler et al 2000) and turfgrasses (Cho et al 2000;Ha et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Expression of green fluorescent protein and its inheritance in transgenic oat plants generated from shoot meristematic cultures

Okamoto

et al. 2003

Plant Cell Rep

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The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating mature seeds of a commercial oat cultivar, Garry, were used as a transformation target. Proliferating SMCs were bombarded with a mixture of plasmids containing the sgfp(S65T) gene and one of three selectable marker genes, phosphinothricin acetyltransferase (bar), hygromycin phosphotransferase (hpt) and neomycin phosphotransferase (nptII). Cultures were selected with bialaphos, hygromycin B and geneticin (G418), respectively, to identify transgenic tissues. From 289 individual explants bombarded with the sgfp(S65T) gene and one of the three selectable marker genes, 23 independent transgenic events were obtained, giving a 8.0% transformation frequency. All 23 transgenic events were regenerable, and 64% produced fertile plants. Strong GFP expression driven by the rice actin promoter was observed in a variety of tissues of the T(0) plants and their progeny in 13 out of 23 independent transgenic lines. Stable GFP expression was observed in T(2) progeny from five independent GFP-expressing lines tested, and homozygous plants from two lines were obtained. Transgene silencing was observed in T(0) plants and their progeny of some transgenic lines.

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“…Because biolistic transformation is a physical process that involves only one biological system, it is a fairly reproducible procedure that can be easily adapted from one laboratory to another laboratory. Transgenic forage plants have been obtained by microprojectile bombardment of embryogenic cells in tall fescue (Spangenberg et al, 1995a;Cho et al, 2000;Wang et al, 2001bWang et al, , 2003aChen et al, 2003Chen et al, , 2004, perennial ryegrass (Spangenberg et al, 1995b;Dalton et al, 1999;Altpeter et al, 2000;Xu et al, 2001;Petrovska et al, 2004;Chen et al, 2005), Italian ryegrass (Ye et al, 1997(Ye et al, , 2001Dalton et al, 1999;Petrovska et al, 2004), orchardgrass (Denchev et al, 1997;Cho et al, 2001), Kentucky bluegrass (Ha et al, 2001;Gao et al, 2006), and Russian wildrye (Wang et al, 2004a) (Table 1).…”

Section: Methods Employed For Transformationmentioning

confidence: 99%

Cool‐Season Forage Grasses

Wang

Yamada

2008

Compendium of Transgenic Crop Plants

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Cool‐season grasses provide most of the forage consumed by ruminant animals in temperate areas of the world. Transgenic plants have been obtained for a number of cool‐season grass species, including tall fescue, perennial ryegrass, Italian ryegrass, orchardgrass, Kentucky bluegrass, and Russian wildrye. This chapter describes the origin, taxonomy, cytological features, economic importance, and biotechnological improvement of these species. Progresses in genetic transformation techniques and the use of transgenic technology for improvement of these species are summarized.

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Stable transformation of a recalcitrant kentucky bluegrass (Poa pratensis L.) cultivar using mature seed-derived highly regenerative tissues

Cited by 32 publications

References 31 publications

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

Expression of green fluorescent protein and its inheritance in transgenic oat plants generated from shoot meristematic cultures

Cool‐Season Forage Grasses

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