Chromosome mapping of genes on the short arm of human chromosome 11: parathyroid hormone gene is at 11p15 together with the genes for insulin, c-Harvey-&lt;i&gt;ras&lt;/i&gt; 1, and β-hemoglobin

Zabel, Bernhard; Kronenberg, Henry M.; Bell, G. I.; Shows, Thomas B.

doi:10.1159/000132135

Cited by 53 publications

(12 citation statements)

References 17 publications

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“…and H R AS l are physically close on rabbit chromosome l . These same loci are closely linked on human chromosome 11 (Zabel et al, 1985). they are syntenic on mouse chromosome 7 (Searle et al.…”

Section: Discussionmentioning

confidence: 96%

Localization of the β-like globin gene cluster and the genes for parathyroid hormone and c-Harvey-<i>ras</i> 1 to region q14→q21 of rabbit chromosome 1 by in situ hybridization

Hardison²

1989

Cytogenet Genome Res

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The rabbit β-like globin gene cluster (HBBC), comprised of Ε-, γ-, δ-, and β-globin genes (HBE, HBG, HBD, and HBB, respectively), has been mapped to chromosome 1, region q14→q21, by in situ hybridization using probes for rabbit HBE and HBG. Probes for the human parathyroid hormone gene (PTH) and the Harvey-mi 1 protooncogene (HRAS1) also localize to this region by in situ hybridization. Thus, these genes are syntenic in lagomorphs as well as four other mammalian orders (primates, rodents, carnivores, and artiodactyls). The mapping data in rabbits provide further evidence that this synteny is strongly conserved in the evolution of mammalian chromosomes.

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Section: Discussionmentioning

confidence: 96%

Localization of the β-like globin gene cluster and the genes for parathyroid hormone and c-Harvey-<i>ras</i> 1 to region q14→q21 of rabbit chromosome 1 by in situ hybridization

Hardison²

1989

Cytogenet Genome Res

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“…However, the coordinate expression of these two gene families is paradoxical. In both humans and rabbits, the two gene families are located on different chromosomes (17,67,68,70) in very different sequence contexts. The a-globin genes are in a very dense, GC-rich isochore (6), are embedded in CpG islands (21,29), and are unmethylated in all tissues examined (7).…”

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confidence: 99%

Nuclear protein-binding sites in a transcriptional control region of the rabbit alpha-globin gene.

1993

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The 5'-flanking and internal regions of the rabbit a-globin gene, which constitute a CpG island, are required for enhancer-independent expression in transfected cells. In this study, electrophoretic mobility shift assays revealed that a battery of nuclear proteins from both erythroid and nonerythroid cells bind specifically to these regulatory regions. Assays based on exonuclease m digestion, methylation interference, and DNase I footprinting identified sequences bound by proteins in crude nuclear extracts and by purified transcription factor Spl. In the 5' flank, recognition sites for the transcription factors a-IRP (positions -53 to -44 relative to the cap site), CP1 (-73 to -69), and Spl (-95 to -90) are bound by proteins in K562 cell nuclear extracts, as are three extended upstream regions. Two recognition sites for Spl in intron 1 are also bound both by proteins in crude nuclear extracts and by purified Spl. The sequences CCAC in intron 2 and C5 in the 3'-untranslated region also bind proteins. A major binding site found in exon 1, TATGGCGC, matches in sequence and methylation interference pattern the binding site for nuclear protein YY1, and binding is inhibited through competition by YY1-specific oligonucleotides. The protein-binding sites flanking and internal to the rabbit a-globin gene may form an extended promoter.

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“…The HRASI-selected transformants were analyzed for cotransfer of each of the nine gene markers shown in Fig. 1 (40b), based on the combined results from deletion and transl9cation mapping in hybrid cells (4,11,12,13,39,42), in situ hybridization analysis of mitotic chromosomes (44), and family linkage data with segregating polymorphic markers (15,41). Cells and culture conditions.…”

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confidence: 99%

Molecular and physical arrangements of human DNA in HRAS1-selected, chromosome-mediated transfectants.

Porteous¹,

Morten²,

Cranston³

et al. 1986

Mol. Cell. Biol.

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We used mitotic chromosomes isolated from a human EJ bladder carcinoma cell line for morphological transformation of mouse C127 cells. These chromosome-mediated transformants were analyzed for cotransfer of markers syntenic with c-Ha-ras-1 on human chromosome 11. We also used cloned, dispersed human DNA repeats, in a general mapping strategy, to quantitate the amounts and molecular state of human DNA transferred along with the activated c-Ha-ras-1 gene. In situ hybridization was used to visualize the physical state of the transfected human chromatin. The combined use of these various techniques revealed the occurrence of both chromosomal and DNA rearrangements. However, our analysis also demonstrated that, in general, very substantial lengths of DNA are transferred intact. Closely linked markers are likely to cosegregate. Therefore, these transformants should be invaluable sources for the complete molecular cloning of isolated fragments of the short arm of human chromosome 11.CMGT is a long-established technique which enables variable subchromosomal lengths of DNA to be transferred efficiently to appropriate recipient cells (reviewed by McBride and Peterson [23]). Exploitation of this and other somatic cell techniques has been compromised by the limited availability of dominantly acting selection systems (23,31,39). We can now describe the use of a new class of dominant selectable markers in CMGT, the human oncogenes. Mitotic chromosomes derived from the human EJ bladder carcinoma cell line induced morphological transformation, anchorage-independent growth, and tumorigenicity in mouse C127 cells

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Chromosome mapping of genes on the short arm of human chromosome 11: parathyroid hormone gene is at 11p15 together with the genes for insulin, c-Harvey-<i>ras</i> 1, and β-hemoglobin

Cited by 53 publications

References 17 publications

Localization of the β-like globin gene cluster and the genes for parathyroid hormone and c-Harvey-<i>ras</i> 1 to region q14→q21 of rabbit chromosome 1 by in situ hybridization

Localization of the β-like globin gene cluster and the genes for parathyroid hormone and c-Harvey-<i>ras</i> 1 to region q14→q21 of rabbit chromosome 1 by in situ hybridization

Nuclear protein-binding sites in a transcriptional control region of the rabbit alpha-globin gene.

Molecular and physical arrangements of human DNA in HRAS1-selected, chromosome-mediated transfectants.

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