Chromosome fragility in lymphocytes of women with cervical uterine lesions produced by human papillomavirus

Paz‐y‐Miño, César; Ocampo, Ligia; Narváez, Romeo; Narváez, Luis Alberto Poalasin

doi:10.1016/0165-4608(92)90211-p

Cited by 14 publications

(8 citation statements)

References 32 publications

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“…Also, cytogenetic abnormalities and numerical and structural aberrations (e.g. endoreduplications, chromatid gaps and breakages, dicentric chromosomes and centric rings) were observed in keratinocytes transfected with the HPV-16 E7 gene and chromosome fragility was detected in lymphocytes from women with cervical uterine lesions with HPV infection after treatment with aphidicolin (Paz y mino et al, 1992). These findings suggest that papillomavirus infections, per se, may cause chromosome fragility.…”

Section: Introductionmentioning

confidence: 59%

Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Stocco

Lindsey

Ferraz

et al. 1998

Journal of General Virology

105

141

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Enzootic haematuria and urinary bladder cancer in cattle are associated with feeding on bracken fern and bovine papillomavirus (BPV) infection. An increased rate of chromosomal aberrations in peripheral blood lymphocytes from chronically affected haematuric cows raised in bracken fern pastures has been reported, suggesting the presence of BPV in the peripheral blood of afflicted animals. The purpose of the present investigation was to examine the role of peripheral blood as a potential BPVtransmitting agent and search for clastogenic effects in experimentally infected animals kept on a bracken fern-free diet. Healthy cows were inoculated with blood samples of haematuric animals every two weeks for 18 months. Recipient cows, their offspring, donor animals and a control group were kept on a bracken fern-free diet throughout the experiment. Clinical and molecular analyses for

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Section: Introductionmentioning

confidence: 59%

Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Stocco

Lindsey

Ferraz

et al. 1998

Journal of General Virology

105

141

View full text Add to dashboard Cite

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“…39 Chromosomal instability, particularly in chromosomes 1, 3, 5, 11 and 17, is associated with the development of cervical carcinoma. 40 The results presented in this study demonstrated that the presence of micronuclei correlated with malignancy, given that the results were significant in relation to various risk factors for cervical cancer.…”

Section: Discussionmentioning

confidence: 82%

Prevalence of micronuclei in exfoliated uterine cervical cells from patients with risk factors for cervical cancer

et al. 2008

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CONTEXT AND OBJECTIVE: Pap smears are the most common and inexpensive screening method for cervical cancer. We analyzed micronucleus prevalence in exfoliated cervical mucosa cells, to investigate associations between increased numbers of micronuclei and risk factors for cervical cancer. DESIGN AND SETTING: Analytical cross-sectional study, at Instituto de Pesquisa em Oncologia (IPON). METHODS: Exfoliated cervical cells were obtained from 101 patients between September 2004 and November 2005. Patients' ages, habits (passive or active smoking, alcoholism and numbers of sexual partners), age at first sexual intercourse, contraceptive methods used, histories of sexually transmitted diseases, use of hormone replacement therapy, numbers of pregnancies and abortions, inflammatory cytology and cervical intraepithelial neoplasia (CIN) were obtained. Cells were collected using Ayre spatulas, transferred to vials containing 0.9% saline solution for micronucleus tests and analyzed at 1000x magnification. The number of micronuclei in 1,000 epithelial cells per patient sample was counted. RESULTS: Comparisons between groups with active (7.9 ± 7.8) and passive (7.2 ± 10.6) smoking versus no smoking (3.7 ± 5.1); with/without alcoholism (7.8 ± 1.4 and 6.9 ± 10.1); with/without inflammatory cytology (10.7 ± 10.5 and 1.3 ± 1.7); and with CIN I, II and III and no CIN (respectively 4.3 ± 4.3, 10.6 ± 5.3, 22.7 ± 11.9 and 1.3 ± 1.4) found elevated micronucleus prevalence (P < 0.05). CONCLUSIONS: We concluded that the prevalence of micronuclei in exfoliated uterine cervical cells was greater in patients with one or more risk factors for uterine cervical cancer than in patients without risk factors.

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“…Cytogenetic abnormalities and numerical and structural chromosome aberrations, such as endoreduplications, gaps, break chromatids, centric rings and dicentric chromosomes were observed in human keratinocytes and mouse cells transfected with E7 oncogene HPV-16. Chromosomal fragility has been detected also in lymphocytes of women with lesions in the cervix after submitted to a treatment against HPV (Paz-y-mino et al, 1992). Campo et al, (1994) and Campo (2002) suggested that the viral latency site occurred in lymphocytes when studying BPV DNA sequences detected in lymphocytes of bovines with or without papillomatosis, as well as HPV DNA in blood cells of women with HPV urogenital and cervical cancer infection.…”

Section: Introductionmentioning

confidence: 98%

Chromosome Aberrations as a Biomarker for Genomic Instability in Cell Cultures Originated From Bovines, Canines and Equines Infected With Papillomavirus

Simões

Barth

2016

Int J Appl Sci Biotechnol

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Chromosomal abnormalities or aberrations were investigated in ten bovines, three canines and three equines presenting cutaneous papillomatosis. The aim of the present study was to evaluate possible chromosomal abnormalities as a biomarker of genomic instability induced by the interaction of the viral genome with the chromatin of the host cell in peripheral blood lymphocytes and skin warts lesion cultures. Genomic DNA was extracted from blood samples and epithelial biopsies. Amplification products from PCR using degenerated primer pair FAP59/FAP64 were sequenced. Peripheral blood lymphocytes and tumor cell cultures were performed. Structural chromosomal aberrations were verified as associations, chromosomal fragments, centric fusions, open chromatids, gaps, rearrangements and rings. Several levels of premature chromosome condensation were observed in both cultures. Numeric abnormalities as aneuploidy were also detected and hipoployd cells were found in a larger number than the hiperployd cells. This cytogenetic study in detecting premature chromosome condensation in the three species investigated describes by the first time the structural and numerical abnormalities in canines and equines presenting papillomatosis lesions. These abnormalities are similar of the three groups of animals, but virus specific.

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Chromosome fragility in lymphocytes of women with cervical uterine lesions produced by human papillomavirus

Cited by 14 publications

References 32 publications

Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Bovine papillomavirus transmission and chromosomal aberrations: an experimental model.

Prevalence of micronuclei in exfoliated uterine cervical cells from patients with risk factors for cervical cancer

Chromosome Aberrations as a Biomarker for Genomic Instability in Cell Cultures Originated From Bovines, Canines and Equines Infected With Papillomavirus

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