Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat

Cainong, Joey C.; Bockus, W. W.; Feng, Yue; Chen, Peidu; Qi, Lili; Sehgal, Sunish K.; Danilova, Tatiana; Koo, Dal‐Hoe; Friebe, Bernd; Gill, Bikram S.

doi:10.1007/s00122-015-2485-1

Cited by 92 publications

(51 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since this region showed a stable response towards FCR at both environmental conditions, these QTLs might be promising candidates for breeding FCR resistance. The genomic regions linked to two QTLs on chromosomes 4BS and 6DS under GR and three QTLs on chromosomes 3BS, 4BS and 6BS under GH were previously identified as resistance QTLs for FHB disease incidence, spread and severity 17,[25][26][27] .…”

Section: Ld Differs Aong 21 Chromosomes In 161 Diverse Wheat Accessionsmentioning

confidence: 99%

Dissecting the Genetic Complexity of Fusarium Crown Rot Resistance in Wheat

Pariyar

Erginbaş-Orakci²,

Dadshani³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Fusarium crown rot (FCR) is one of the most important diseases of wheat (Triticum aestivum L.). fcRis mainly caused by the fungal pathogens Fusarium culmorum and F. pseudograminearum. In order to identify new sources of resistance to FCR and to dissect the complexity of FCR resistance, a panel of 161 wheat accessions was phenotyped under growth room (GR) and greenhouse conditions (GH). Analysis of variance showed significant differences in crown rot development among wheat accessions and high heritability of genotype-environment interactions for GR (0.96) and GH (0.91). Mixed linear model analysis revealed seven novel quantitative trait loci (QTLs) linked to F. culmorum on chromosomes 2AL, 3AS, 4BS, 5BS, 5DS, 5DL and 6DS for GR and eight QTLs on chromosomes on 3AS, 3BS, 3DL, 4BS (2), 5BS, 6BS and 6BL for GH. Total phenotypic variances (R²) explained by the QTLs linked to GR and GH were 48% and 59%, respectively. In addition, five favorable epistasis interactions among the QTLs were detected for both GR and GH with and without main effects. Epistatic interaction contributed additional variation up to 21% under GR and 7% under GH indicating strong effects of environment on the expression of QTLs. Our results revealed FCR resistance responses in wheat to be complex and controlled by multiple QTLs.www.nature.com/scientificreports www.nature.com/scientificreports/ of inheritance, and strong genotype-by-environment interaction 20-23 . Further, molecular mechanisms of FCR resistance and interaction with environment are poorly understood. Thus, characterizing potential epistatic interactions between QTLs and environment will provide new insight in understanding the genetic basis of FCR resistance.Wheat accessions of different origin comprise a broad genetic diversity which can be exploited to breed for high yielding and disease resistant cultivars. Current progress in high density mapping, sequence availability and statistical tools make it possible to uncover the majority of QTL effects and functionally characterize their role in host-pathogen interactions. Genome-wide association study (GWAS) is a powerful approach to detect associations between phenotypic variation and genetic polymorphisms; in this way, QTLs for traits such as FCR can be located in the genome. GWAS utilizes linkage disequilibrium to dissect the genetic architecture of complex traits by correlating phenotypes to genotypes. It has been used earlier to identify QTLs that are involved in plant responses to soil borne pathogens in a large set of unrelated wheat accessions 19,24 . Detection of QTLs tightly linked to gene/s that control FCR resistance are the pre-requisite for marker assisted breeding. In the present study, we analyze the genetic basis of the FCR infection, disease development, and the interaction accross multiple environments. Our study not only confirmed previously described QTLs linked to FCR but also identified novel QTLs that have additive main and epistatic effects on FCR resistance which upon functional validation would provide addit...

show abstract

Section: Ld Differs Aong 21 Chromosomes In 161 Diverse Wheat Accessionsmentioning

confidence: 99%

Dissecting the Genetic Complexity of Fusarium Crown Rot Resistance in Wheat

Pariyar

Erginbaş-Orakci²,

Dadshani³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Development of only one or a few sources of resistance over large crop production areas poses vulnerability to resistance breakdown and disease epidemics. Wangshuibai, and Fhb6 derived from Elymus tsukushiensis have been mapped and designated previously (Cuthbert et al 2006(Cuthbert et al , 2007Liu et al 2006;Qi et al 2008;Xue et al 2010Xue et al , 2011Cainong et al 2015). Numerous quantitative trait loci (QTL) have been reported as associated with FHB resistance (reviewed by Buerstmayr et al 2009).…”

Section: Introductionmentioning

confidence: 99%

High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection

et al. 2015

View full text Add to dashboard Cite

Wheat lines with shortened Th. ponticum chromatin carrying Fhb7 and molecular markers linked to Fhb7 will accelerate the transfer of Fhb7 to breeding lines and provide an important resource for future map-based cloning of this gene. Fusarium head blight is a major wheat disease globally. A major FHB resistance gene, designated as Fhb7, derived from Thinopyrum ponticum, was earlier transferred to common wheat, but was not used in wheat breeding due to linkage drag. The aims of this study were to (1) saturate this FHB resistance gene region; (2) develop and characterize secondary translocation lines with shortened Thinopyrum segments carrying Fhb7 using ph1b; (3) pyramid Fhb7 and Fhb1 by marker-assisted selection. Fhb7 was mapped in a 1.7 cM interval that was flanked by molecular markers XsdauK66 and Xcfa2240 with SSR, diversity arrays technology, EST-derived and conserved markers. KS24-2 carrying Fhb7 was analyzed with molecular markers and genomic in situ hybridization, confirming it was a 7DS.7el2L Robertsonian translocation. To reduce the Thinopyrum chromatin segments carrying Fhb7, a BC1F2 population (Chinese Spring ph1bph1b*2/KS24-2) was developed and genotyped with the markers linked to Fhb7. Two new translocation lines (SDAU1881 and SDAU1886) carrying Fhb7 on shortened alien segments (approximately 16.1 and 17.3% of the translocation chromosome, respectively) were developed. Furthermore, four wheat lines (SDAU1902, SDAU1903, SDAU1904, and SDAU1906) with the pyramided markers flanking Fhb1 and Fhb7 were developed and the FHB responses indicated lines with mean NDS ranging from 1.3 to 1.6 had successfully combined Fhb7 and Fhb1. Three new molecular markers associated with Fhb7 were identified and validated in 35 common wheat varieties. The translocation lines with shortened alien segments carrying Fhb7 (and Fhb1) and the markers closely linked to Fhb7 will be useful for improving wheat scab resistance.

show abstract

“…tsukushiensis chromosome 1Ets#1S added to the wheat genome conferred resistance to FHB. Cainong et al [72] replaced corresponding homeologous region of chromosome 1AS of wheat with the FHB resistance-associated chromatin derived from 1Ets#1S of E. tsukushiensis. Plant progenies homozygous for such chromosome fragment had a disease severity rating of 7% compared to 35% for the null progenies.…”

Section: Utilization Of Alien Genesmentioning

confidence: 99%

Breeding for the resistance to Fusarium head blight of wheat in China

Ma¹,

Zhang²,

Jinbao³

et al. 2019

Front. Agr. Sci. Eng.

View full text Add to dashboard Cite

With the changes of climate and cultivation systems, the Fusarium head blight (FHB) epidemic area in China has extended since 2000 from the reaches of the Yangtze River to the north and west winter wheat region. Breeding for FHB resistance in wheat is an effective way to control the disease. Chinese wheat breeders commenced research on FHB in the 1950s. Sumai 3, Ning 7840, Yangmai 158, Ningmai 9 and other cultivars with improved FHB resistance were developed through standard breeding methods and widely applied in production or breeding programs. In addition to intervarietal crosses, alien germplasm was used to improve FHB resistance of wheat. Addition, substitution and translocation lines with alien chromosomes or chromosome fragments were created to enhance FHB resistance. Somaclonal variation was also used to develop a FHB resistant cv. Shengxuan 3 and other cultivars with moderate resistance to FHB were released by such methods. QTL (quantitative trait loci) for FHB resistance were characterized in cultivars originating from China. The major QTL, Fhb1, was identified on chromosome 3BS in Sumai 3, Ning 894037, Wangshuibai and other Chinese resistant sources. Diagnostic molecular markers for Fhb1 have been applied in wheat breeding and breeding lines with improved FHB resistance and desirable agronomic traits have been obtained. However, breeding for FHB resistance is a long-term task, new technologies are likely to increase the efficiency of this process and better FHB resistance of new cultivars is expected to be achieved within the next decade.

show abstract

Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat

Cited by 92 publications

References 24 publications

Dissecting the Genetic Complexity of Fusarium Crown Rot Resistance in Wheat

Dissecting the Genetic Complexity of Fusarium Crown Rot Resistance in Wheat

High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection

Breeding for the resistance to Fusarium head blight of wheat in China

Contact Info

Product

Resources

About