Chromosome C-banding of Zea mays and its closest relatives

Abstract

MASTENBROEK, I., and J . M. J . DE WET. 1983. Chromosome C-banding of Zecl mays and its closest relatives. Can. J . . An established C-banding technique was modified to consistently yield complete sets of distinct, sharply banded chromosomes in Zea. It was used to demonstrate similarities and differences among heterochromatin patterns of different Zea taxa. The C-banding patterns showed a general agreement with heterochromatic knob positions known from pachytene studies. Two groups of banding patterns could be distinguished. Those taxa with bands that were almost exclusively terminally positioned included Z . mays ssp. pnrviglumis var. huehuerennngensis, Z . diploperennis, Z . perennis, and Z . luxurians. In the last mentioned, previously unknown heterochromatic regions were found. Both terminal and subterminal band positions were found in Z . mays, Z . mays ssp. mexicana, and Z . mays ssp. parvig1umi.s var. parviglurnis. On the basis of these results, and those of other workers, the taxonomic treatments of Z . mays ssp. parviglumis var. huehuerennngensis, and of Z . diploperennis and its autotetraploid derivative Z . perennis are questioned. MASTENBROEK, I., et J. M. J. DE WET. 1983. Chromosome C-banding of Zea mc1y.s and its closest relatives. Can. J. Genet. Cytol. 25: 203-209. Une technique connue d'application des bandes C etait modifie jusque c'dtait capable de produire consistant un ensemble complet et distinct des chromosomes avec des bandes nette. Elle dtait utilisk pour demonstrer des similarites et des differences parmi les arrangements de h6terochromatine des taxons differentes de Zecl. Les arrangements de band C revelaient une concordance genkrale avec les positions des blocs d'heterochromatine connu des etudes pachytenique. Deux groups d'arrangements des bandes etaient distingue. Les taxons avec bandes presque exclusivement aux positions terminales sont Z . mays ssp. parviglumis var. huehuerennngen.si.s, Z . dip1operenni.s. Z . perennis et Z . 1u.uurinns. Dans la derniere des regions d'heterochromatine etait decouvert qui etaient inconnue. A la fois des bandes terminales et subterminales etaient decouvert en Z . mays, Z. mays ssp. me-uicann et Z . mays ssp. pnr\iglumis var. huehue~enc~izgensis. A cause de nos resultats, et ceux d'autre chercheurs, le traitement taxonomic de Z . mays ssp. par\~i~lumi.s var. huehuerenc~ngen.si.s, et de Z . diploperennis et son derive autotetraploid Z . perennis sont mis en question.

A simple chromosomal marker can reliably distinguishes Poncirus from Citrus species

“…In wheat and related species, for example, Shang et al (1989) reported heterochromatic band size polymorphisms for all accessions investigated. Similar results were verified in maize and barley (Mastenbroek and de Wet 1983;Kakeda et al 1991).…”

Nuclear DNA content in the genera Zea and Sorghum. Intergeneric, interspecific and intraspecific variation

“…First, the Knobless Tama Flint (KTF), which is devoid of detectable C-band positive material (Mastenbroek and de Wet, 1983;Rayburn et aL, 1985), was found to have a significantly higher DNA content than Gaspé Flint, which has 4 C-bands (Rayburn et a!., 1985). Results from the present study suggest that KFT also has a higher DNA content than Seneca 60, although the KTF measurements were unreplicated.…”

“…Results from the present study suggest that KFT also has a higher DNA content than Seneca 60, although the KTF measurements were unreplicated. Second, Z. luxurians has by far the highest DNA content of the diploid Zeas but does not appear to have sufficient heterochromatin to account for this difference (Mastenbroek and de Wet, 1983). …”

A new heterochromatin banding pattern revealed by modified HKG banding technique in maize chromosomes

“…However, inconsistency of stainability in obtaining C-band involving centromeric A heterochromatin has been reported (Hadlaczky & Kalman, 1975;Ward, 1980;Chow & Larter, 1981;Mastenbroek & De Wet, 1983;Aguiar-Perecin, 1985).…”