Chromosome assignment of monoclonal antibody‐defined determinants on human leukemic cells

Katz, F; Povey, S.; Parkar, MH; Schneider, Claudio; Sutherland, Robert; Stanley, Karina; Solomon, Ellen; Greaves, Melvyn F.

doi:10.1002/eji.1830131211

Cited by 51 publications

(23 citation statements)

References 39 publications

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“…A. Fantes, H. Morrison, and V.v.H., unpublished data; A.S., J.M.F., D.J.P., N.D.H., and V.v.H., unpublished data). 1B-8-1/6 is a mouse-human somatic cell hybrid whose sole human component is chromosome 11 (22). The HRASJselected CMGT cell line E67-1 was derived by morphological transformation of mouse C127 cells (23), with mitotic chromosomes isolated from the human bladder carcinoma cell line EJ-18-8D (24), as described (15,16).…”

Section: Methodsmentioning

confidence: 99%

HRAS1-selected chromosome transfer generates markers that colocalize aniridia- and genitourinary dysplasia-associated translocation breakpoints and the Wilms tumor gene within band 11p13.

Porteous¹,

Bickmore²,

Christie³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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We show that chromosome-mediated gene transfer can provide an enriched source of DNA markers for predetermined, subchromosomal regions of the human genome. Forty-four human DNA recombinants isolated from a HRASI-selected chromosome-mediated gene transformant map exclusively to chromosome 11, with several sublocalizing to the Wilms tumor region at llpl3. We present a detailed molecular map of the deletion chromosomes 11 from five WAGR (Wilms tumor/aniridia/genitourinary abnormalities/ mental retardation) syndrome patients, three of which are at the limits of cytogenetic resolution but shown here to be molecularly distinguishable and overlapping. We can define ten distinct regions of the short arm of chromosome 11, five of which subdivide band lipl3. We also map two independent llpl3 translocation breakpoints to within the smallest region of overlap defined by the WAGR deletions. The first comes from a patient with familial aniridia, and the second from a patient with Potter facies and genitourinary dysplasia. The close similarities in map location and affected cell lineage for Wilms tumor and genitourinary dysplasia suggest that they may be alternative manifestations of mutation at the same locus.The association of aniridia, genitourinary abnormalities, and mental retardation with Wilms tumor (WAGR syndrome; genetic locus WAGR) is well established (1, 2). Cytogenetic analysis of independent WAGR syndrome patients has localized the relevant genetic region to band llpl3 (3). We (4) and others (5-8) have utilized available DNA probes and expressed protein markers to start to define the molecular extent of the various deletions. In most studies, only one patient has been examined (5-7) or the deletions have been large and therefore likely to show loss of markers that map some distance from the disease-related genes (8). We now attempt to distinguish the smaller deletions, those which take us beyond the resolving power of cytogenetic analysis towards defining the smallest region of overlap that is within reach of complete molecular analysis. We make use of five independent WAGR deletion hybrids, three of which are at the limit of cytogenetic resolution and involve only part of band llpl3.The short arm of human chromosome 11 (lip) is one of the better mapped regions of the genome (9, 10), but there is still a relative dearth of genetic markers that map within even the larger WAGR syndrome deletions (4, 8). The genes encoding erythrocyte catalase (CAT) and two cell surface markers, MIC4 and MIC11 (synonymous with MIC1), all map to band llpl3 and are closely associated with WAGR but not invariably deleted (4, 8). Most recently (8), the /3 subunit of follicle-stimulating hormone (FSHB) has been shown to map close to the aniridia and Wilms tumor genes. Of the anonymous DNA markers mapped to the short arm of chromosome 11, the majority derive from a somatic cell hybrid considered to retain lip material as the sole human component (11). However, none of these markers has been found to map as close to WAGR as CAT or F...

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Section: Methodsmentioning

confidence: 99%

HRAS1-selected chromosome transfer generates markers that colocalize aniridia- and genitourinary dysplasia-associated translocation breakpoints and the Wilms tumor gene within band 11p13.

Porteous¹,

Bickmore²,

Christie³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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“…The genetic side of the human CD38 story began in 1983 when the gene was assigned to chromosome 4 by using the OKT10 mAb to detect expression of the molecule on murine:human somatic hybrids [61]. Twelve years later, this assignment was refined to the short arm of the chromosome at 4p15 [62].…”

Section: The Human Cd38 Genementioning

confidence: 99%

The metamorphosis of a molecule: from soluble enzyme to the leukocyte receptor CD38

Ferrero

Malavasi

1999

Journal of Leukocyte Biology

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Human CD38 is a 45-kDa type II membrane glycoprotein with an intricate pattern of expression in leukocytes, although evidence is accumulating of its quite widespread expression in cells of nonvascular origin. CD38 is a member of a nascent eukaryotic gene family encoding cytosolic and membrane-bound enzymes whose substrate is NAD, a coenzyme ubiquitously distributed in nature. Functionally, CD38 is an eclectic molecule with the ability not only to catalyze but also to signal, to mobilize calcium, and to adhere to itself, to hyaluronan, and to other ligands. Interaction with CD38 on various leukocyte subpopulations has profound though diverse consequences on their life-span, but these effects seem to be independent of the enzymatic activity of the molecule. CD38 challenges our expectations of a surface molecule and we must sift through its many guises to unmask its true nature. J. Leukoc. Biol. 65: 151-161; 1999.

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“…In addition to the genes MSK3 and MSK4 listed for chromosome 12 in HGM7 MSK7 coding for a protein determined by monoclonal antibody VI, MSK27 which determines the expression of MG 6 , and MJC3 identified by monoclonal antibody BA-2 (Katz et al 1983) are located on chromosome 12. Dracopoli et al (HGM8) now present information on subregional mapping of these genes: MSK 27 to 12cen--qter, MSK4 to 12cen--ql3, MSK7 to 12ql3--qter, and MIC3 to 12cen--pter.…”

Section: Chromosome 11mentioning

confidence: 99%

Report of the committee on the genetic constitution of chromosomes 10, 11, and 12

Grzeschik¹,

Kazazian²

1985

Cytogenet Genome Res

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Chromosome assignment of monoclonal antibody‐defined determinants on human leukemic cells

Cited by 51 publications

References 39 publications

HRAS1-selected chromosome transfer generates markers that colocalize aniridia- and genitourinary dysplasia-associated translocation breakpoints and the Wilms tumor gene within band 11p13.

HRAS1-selected chromosome transfer generates markers that colocalize aniridia- and genitourinary dysplasia-associated translocation breakpoints and the Wilms tumor gene within band 11p13.

The metamorphosis of a molecule: from soluble enzyme to the leukocyte receptor CD38

Report of the committee on the genetic constitution of chromosomes 10, 11, and 12

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