Chromosome abnormality rates in human embryos obtained from in-vitro maturation and IVF treatment cycles

Zhang, Xiao Yun; Ata, Barış; Son, Weon‐Young; Buckett, William; Tan, Seang‐Lin; Ao, Asangla

doi:10.1016/j.rbmo.2010.05.002

Cited by 66 publications

(35 citation statements)

References 24 publications

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“…IVM timing was also a factor with respect to the induction of chromosome aneuploidy of IVM embryos. Zhang et al studied the chromosome abnormality rates in human embryos obtained from in vitro maturation and IVF treatment cycles, and they found that the aneuploidy will be significantly increased in embryos derived from oocytes that matured 48 h after collection compared with oocytes matured 24 h or the control group [50]. Emery et al also reported that the incidence of aneuploidy in embryos with delayed fertilization for IVM oocytes would reach approximately 80%, which was significantly higher than in the control group [51].…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection

Zhao

et al. 2014

PLoS ONE

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Implantation failure and early pregnancy loss have been reported to be closely related to the quality of mammalian oocytes; however, the pregnant outcome of embryos from in-vitro matured (IVM) oocytes remains unknown. In this study we examined spindle assembly and chromosome segregation during differentiation, and the duration of IVM of mouse oocytes. The resulting implantation and pregnancy outcomes were analyzed to clarify the relationship between the spindle and chromosomes of IVM oocytes and implantation and early pregnancy. Cumulus-enclosed germinal vesicle oocytes were collected and randomly cultured in IVM medium with different IVM durations. One part of IVM oocytes were analyzed the spindle and chromosome morphology by immunofluorescence method, and the other part of them were fertilized by intracytoplasmic sperm injection. The resulting embryos were transferred into pseudo-pregnant female mice, and the post-implantation and full term development was observed. The chromosome aberrations and incorrect spindle assembly seems not affect the early development and blastocyst cell number derived from IVM oocytes, however the development potential of the resulting embryos after implantation were significant decreased with the ratio increasing of chromosome aberrations and incorrect spindle assembly. Accordingly, the full-term development was also decreased. In conclusion, the present study showed the spindle assembly of in vitro-matured oocytes was one of the most important factors that affected the implantation and ongoing pregnancy rates of IVM oocytes, and the improvement by an appropriate duration of maturation in vitro will enhance the post-implantation development potential of the resulting embryos, and decrease implantation failure and early pregnancy loss.

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Section: Discussionmentioning

confidence: 99%

Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection

Zhao

et al. 2014

PLoS ONE

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“…The clinical and embryology laboratory IVM protocols exercised in our unit are described in elsewhere [10,11]. Briefly all women underwent a transvaginal ultrasound scan on the 2 nd or 3 rd day of a spontaneous or progestin induced menstrual bleeding to exclude ovarian cysts and endometrial pathology.…”

Section: Ivm Protocolmentioning

confidence: 99%

“…In Vitro Maturation (IVM) treatment involves collection of oocytes relatively earlier in the follicular phase when the majority is expected to be still immature and therefore does not require Controlled Ovarian Hyperstimulation (COS) [10,11]. Different follicular dynamics, the shorter duration of proliferative phase and the lack of exposure of endometrium to supraphysiological estrogen levels can lead to a different endometrial environment in IVM cycles than in conventional COS -IVF cycles.…”

Section: Introductionmentioning

confidence: 99%

Effect of Body Mass Index on In Vitro Maturation Treatment Outcomes in Women without Polycystic Ovarian Syndrome

Ata¹

2013

J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol

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Purpose: Previous studies have suggested that obesity can affect the oocyte yield, embryo quality and pregnancy rates following IVF. In vitro maturation differs from IVF in several ways including lack of controlled ovarian stimulation, oocyte retrieval when majority are expected to be still immature, and possibly different endometrial environment due to short secretory phase and the lack of exposure to supraphysiologic estrogen levels. Therefore female obesity can affect IVM and IVF cycles differentially. This study aims to assess the effect of female obesity on IVM outcomes in women without polycystic ovarian syndrome. Methods:Retrospective chart review of 125 women who underwent an IVM cycle during a 5 year period. Women were divided to four categories based on Body Mass Index (BMI) according to the World Health Organization classification. Procedural and clinical outcomes were compared across different BMI categories. Independent effect of BMI on the odds of achieving a live birth was assessed using a multivariate logistic regression model. Results:The numbers of cumulus corona complexes, in vivo matured and immature oocytes collected, metaphase two oocytes available for fertilization, good quality embryos available for transfer and embryos transferred were not statistically significantly different across BMI categories. Likewise, in vitro maturation rate of immature oocytes, fertilization rate, embryo implantation, clinical pregnancy and live birth rates were similar across the groups. BMI category did not have an independent effect after adjusting for possible confounders in the regression model. Conclusions:BMI does not seem to affect IVM outcomes.

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“…Embryos frozen on day 2 were cultured for an additional day after thawing before the blastomere biopsy was performed as described previously [19,20]. Embryos frozen on day 3 were thawed in the morning and biopsied on the same day after culturing for a few hours for equilibration.…”

Section: Vitrification and Warming Proceduresmentioning

confidence: 99%

Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy

Zhang

Yılmaz

Chian

et al. 2011

J Assist Reprod Genet

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Chromosome abnormality rates in human embryos obtained from in-vitro maturation and IVF treatment cycles

Cited by 66 publications

References 24 publications

Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection

Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection

Effect of Body Mass Index on In Vitro Maturation Treatment Outcomes in Women without Polycystic Ovarian Syndrome

Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy

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