Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy

Zhang, Li; Yılmaz, Ahmet; Chian, Ri‐Cheng; Son, Weon‐Young; Zhang, Xiao Yun; Kong, Dewen; Dahan, Michael H.; Holzer, Hannanel; Tan, Seang Lin; Ao, Asangla

doi:10.1007/s10815-011-9556-2

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…For women with fewer than three good-quality embryos, embryo transfer was at Day 2-3. Supernumerary good-quality embryos were cryopreserved (Zhang et al, 2011). The number of embryos transferred was determined according to the provincial guidelines at that time: up to two or three embryos and never more than three.…”

Section: Embryo Culturementioning

confidence: 99%

Poor ovarian response as a predictor for live birth in older women undergoing IVF

Cohen

Tannus

Alzawawi

et al. 2018

Reproductive BioMedicine Online

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Women of advanced age present a major challenge for fertility treatments. This study was designed to assess whether poor ovarian response (POR) according to the Bologna criteria is a significant predictor for live birth in women over 40. The outcomes of subsequent IVF cycles were also studied. The results of 1870 fresh IVF cycles in 1212 women were retrospectively analysed. The live birth per cycle was 3.3 times higher (11.61% versus 3.54%, P < 0.001) in good responders with more than three oocytes collected compared with women with less. Ovarian response defined by oocytes collected, but not by the number of follicles, was independently associated with live birth (odds ratio, 2.0; 95% confidence interval, 1.18 to 3.54; P = 0.009). The occurrence of POR in subsequent IVF cycles was only 55%. No differences in live births were found in persistent POR compared with women with at least one good response. A single episode of POR in a first IVF cycle in older women has a limited predictive value for the outcomes of subsequent cycles. POR in women aged 40-43 years, defined by the number of oocytes retrieved, is a predictor for live birth in IVF.

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Section: Embryo Culturementioning

confidence: 99%

Poor ovarian response as a predictor for live birth in older women undergoing IVF

Cohen

Tannus

Alzawawi

et al. 2018

Reproductive BioMedicine Online

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“…Blastocyst hatching is an important step in the sequence of physiologic events that end up in implantation [ 36 ]. Previous studies demonstrated that the small hole that must be created in the zona for embryo biopsy after thawing may also help the embryo to hatch [ 37 ]. Although hatching effect on clinical outcomes remains unknown, some studies hypothesized that a fully hatching embryo was more friable and less likely to implant that a non-fully hatching embryo [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Freeze all-first versus biopsy-first: A retrospective analysis of frozen blastocyst transfer cycles with preimplantation genetic testing for aneuploidy

Park

Choe

et al. 2022

PLoS ONE

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Potential use of preimplantation genetic testing for aneuploidy (PGT-A) is increasing. Patients who have excess embryos cryopreserved at the blastocyst stage may desire PGT-A but there is little data available on options for these patients. We compared the efficacy and safety of the timing on the cryopreservation and trophectoderm(TE) biopsy for preimplantation genetic testing for aneuploidy (PGT-A) program associated with the better outcomes after frozen blastocyst transfer. Retrospective analysis of patients who underwent PGT-A cycles from January 2016 to December 2019 was carried out. 2684 blastocysts from cycles were subjected to TE biopsy for performing array comparative genomic hybridization test and Next-generation sequencing. All cycles were divided into two according to the timing of biopsy: biopsy-first (n = 211 cases/ 232 transfers) versus freeze all-first (n = 327 cases/ 415 transfers). In the biopsy-first group, embryos were cultured to expanded blastocyst and proceed to TE biopsy on day 5 or day 6 followed by cryopreservation. In the freeze all-first, blastocysts were vitrified and warmed before biopsy. Rates of clinical pregnancy (52.3% vs. 38.7%, P = 0.09) and ongoing pregnancy (44.3% vs. 34.5%, P = 0.07) in biopsy-first were significantly higher than those in freeze all-first. Biopsy-first showed comparable miscarriage rate with freeze all-first (15.2% (33/217) vs.11.1% (10/90), respectively). Rate ratio (RR) for clinical pregnancy was lower in freeze all-first group (adjusted RR = 0.78, 95% confidence interval: 0.65, 0.93). The RRs for miscarriage and live birth was also lower but it did not reach statistical significance. Our result supported performing TE biopsy of blastocyst for PGT-A before vitrification and warming. This finding would contribute to more evidence-based decision in PGT-A cycles.

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“…Embryonic development is assessed and the embryos are vitrified 2 days after ICSI. A previous study showed that freezing embryos on day 2 or 3 post-fertilization doesn't have any significant effect on embryo survival and PGD test success rates [10,24].…”

Section: Methodsmentioning

confidence: 99%

Live birth following serial vitrification of embryos and PGD for fragile X syndrome in a patient with the premutation and decreased ovarian reserve

Nayot

Chung

Son

et al. 2013

J Assist Reprod Genet

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Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy

Abstract: Reliable genetic diagnosis can be performed in thawed human embryos vitrified without biopsy. However, further research is required to support this conclusion.

Cited by 4 publications

References 26 publications

Poor ovarian response as a predictor for live birth in older women undergoing IVF

Poor ovarian response as a predictor for live birth in older women undergoing IVF

Freeze all-first versus biopsy-first: A retrospective analysis of frozen blastocyst transfer cycles with preimplantation genetic testing for aneuploidy

Live birth following serial vitrification of embryos and PGD for fragile X syndrome in a patient with the premutation and decreased ovarian reserve

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