Chromosome aberration assays in Pisum for the study of environmental mutagens

Grant, W. F.; Owens, Elizabeth

doi:10.1016/s1383-5742(00)00064-8

Cited by 37 publications

(21 citation statements)

References 118 publications

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“…The observed aberrations have reflected the influence of zinc, lead and cadmium ions, whose concentrations were markedly higher in the study site as compared to the area colonized by the control population. The activity of heavy metal ions, inducing chromosomal aberrations in plants in controlled conditions, has been demonstrated by, e.g., El-Ghamery et al (2003); Liu et al (1995); Pahlsson (1990); Duan and Wang (1995); Kristen et al (1997); Das et al (1998); Grant and owens (2001). Our study is one of few reports demonstrating chromosomal aberrations and disturbed mitotic divisions in plants and linking the phenomena to genetic structure of the individuals in nature.…”

Section: Discussionsupporting

confidence: 67%

Effects of heavy metal pollution on genetic variation and cytological disturbances in thePinus sylvestris L. population

et al. 2006

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This isoenzymatic and cytogenetic study has shown significant differences in genetic composition between two groups of Pinus sylvestris trees: tolerant and sensitive to heavy metal pollution. Total and mean numbers of alleles and genotypes per locus were higher in the pollution-sensitive group of trees, but heterozygosity (Ho) was lower in this group. Fixation index (F) indicates that trees tolerant for pollution were in the Hardy-Weinberg equilibrium, while the sensitive group had a significant excess of homozygosity. Cytological analyses demonstrated numerous aberrations of chromosomes in meristematic root tissue of seedlings developed from seeds collected from trees in the polluted area. The aberrations included chromosome bridges and stickiness, laggards, retarded and forward chromosomes, and their fragments. The mitotic index was markedly lower in this group of seedlings, as compared to the control. Both isoenzymatic and cytological analyses showed a significant influence of heavy metal ions on the genetic structure of the Pinus sylvestris population.

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Section: Discussionsupporting

confidence: 67%

Effects of heavy metal pollution on genetic variation and cytological disturbances in thePinus sylvestris L. population

et al. 2006

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“…The Trifolium repens leaf cell nuclei were found to be more convenient and authentic than other methods because in this method the plant accumulate more metabolites in its leaves, as T. repens is a medicinal plant and the essential parts being the flowers and leaves from which different chemicals work against the various ailments. The operation such as the preparation of microscopic slides was more authentic and throws light on DNA damage cytologically [20, 21]. …”

Section: Discussionmentioning

confidence: 99%

“…The present study also throws light that, if heavy metals from autobody refining shops, will remain unchecked can accumulate in the food chain, as Trifolium repens is grown as a medicinal and pasture plant consumed by the live stock, on which humans depend for milk, meat, and so forth [20, 22]. …”

Section: Discussionmentioning

confidence: 99%

Synergistic Cytotoxic Stress and DNA Damage in Clover (Trifolium repens) Exposed to Heavy Metal Soil from Automobile Refining Shops in Kashmir-Himalaya

et al. 2011

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Coexposure to heavy metals occurs in many occupational settings, such as automobile refining shops, pigment, and batteries production. Heavy metals around automobile refining shops were tested for their ability to induce synergistic cytogenetic effects in Trifolium repens L. by using the chromosomal aberrasions (CAs), micronucleus (MN) and comet assay. A significant increase in micronucleus (MN), chromosomal abrations (CAs), percentage of nuclei with comet tails (NCTs), the relative comet tail length (CTL), comet tail DNA (CT, DNA), and tail moment (TM) were observed with increased concentration of three heavy metals, like Cd, Pb, Hg. The present result indicate that exposure of T. repens to soils contaminated by heavy metals around automobile refining shops shows clastogenicity, cytotoxicity, and DNA damage at higher concentrations.

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“…Grant and Owens [81] showed that atrazine induced chromosome breaks (in mitosis and meiosis) in the species Pisum sativum and Allium cepa.…”

Section: Atrazinementioning

confidence: 99%

Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health

Marin-Morales¹,

Camargo²,

Hoshina³

2013

Herbicides - Current Research and Case Studies in Use

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Chromosome aberration assays in Pisum for the study of environmental mutagens

Cited by 37 publications

References 118 publications

Effects of heavy metal pollution on genetic variation and cytological disturbances in thePinus sylvestris L. population

Effects of heavy metal pollution on genetic variation and cytological disturbances in thePinus sylvestris L. population

Synergistic Cytotoxic Stress and DNA Damage in Clover (Trifolium repens) Exposed to Heavy Metal Soil from Automobile Refining Shops in Kashmir-Himalaya

Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health

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