Synergistic Cytotoxic Stress and DNA Damage in Clover (<i>Trifolium repens</i>) Exposed to Heavy Metal Soil from Automobile Refining Shops in Kashmir-Himalaya

Bhat, Towseef Mohsin; Ansari, Mohd Ikram; Choudhary, Sana; Aslam, Rumana

doi:10.5402/2011/109092

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…For this purpose, the widely distributed species, Trifolium repens (white clover) was used as a model for the ecotoxicological study of heavy metals (Manier et al 2012;Mohsin Bhat et al 2011;Ghiani et al 2014). White clover were grown under the following conditions: (i) plant growth in field soil, (ii) typical environmental concentrations of Cd and Pb, (iii) periods of exposure compatible with the plant's lifetime and with the time of occurrence of any genotoxic events, as well as their long-term consequences possible, (iv) possible combined presence of Cd and Pb since they are often found together in contaminated soils.…”

Section: Introductionmentioning

confidence: 99%

Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens

Lanier¹,

Bernard²,

Dumez³

et al. 2015

Environ Sci Pollut Res

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The present study was designed to investigate the combined effects of Cd and Pb on accumulation and genotoxic potential in white clover (Trifolium repens). For this purpose, T. repens was exposed to contaminated soils (2.5-20 mg kg(-1) cadmium (Cd), 250-2000 mg kg(-1) lead (Pb) and a mixture of these two heavy metals) for 3, 10 and 56 days. The resulting bioaccumulation of Cd and Pb, DNA damage (comet assay) and peroxidase activities (APOX and GPOX) were determined. The exposure time is a determinant factor in experiments designed to measure the influence of heavy metal contamination. The accumulation of Cd or Pb resulting from exposure to the two-metal mixture does not appear to depend significantly on whether the white clover is exposed to soil containing one heavy metal or both. However, when T. repens is exposed to a Cd/Pb mixture, the percentage of DNA damage is lower than when the plant is exposed to monometallic Cd. DNA damage is close to that observed in the case of monometallic Pb exposure. Peroxidase activity cannot be associated with DNA damage under these experimental conditions.

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Section: Introductionmentioning

confidence: 99%

Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens

Lanier¹,

Bernard²,

Dumez³

et al. 2015

Environ Sci Pollut Res

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show abstract

“…These authors showed a dose-dependent increase in DNA damage. More recently, Cd-induced DNA degradations were also observed in Trifolium repens (Bhat et al, 2011 ), Lactuca sativa (Monteiro et al, 2012 ), Lupinus luteus (Arasimowicz-Jelonek et al, 2012 ), Vigna unguiculata (Amirthalingam et al, 2013 ), N. tabacum (Tkalec et al, 2014 ), V. faba and A. cepa (Arya and Mukherjee, 2014 ). However, dose-dependent responses were not clearly observed in these studies.…”

Section: Metalsmentioning

confidence: 95%

The use of comet assay in plant toxicology: recent advances

2015

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The systematic study of genotoxicity in plants induced by contaminants and other stress agents has been hindered to date by the lack of reliable and robust biomarkers. The comet assay is a versatile and sensitive method for the evaluation of DNA damages and DNA repair capacity at single-cell level. Due to its simplicity and sensitivity, and the small number of cells required to obtain robust results, the use of plant comet assay has drastically increased in the last decade. For years its use was restricted to a few model species, e.g., Allium cepa, Nicotiana tabacum, Vicia faba, or Arabidopsis thaliana but this number largely increased in the last years. Plant comet assay has been used to study the genotoxic impact of radiation, chemicals including pesticides, phytocompounds, heavy metals, nanoparticles or contaminated complex matrices. Here we will review the most recent data on the use of this technique as a standard approach for studying the genotoxic effects of different stress conditions on plants. Also, we will discuss the integration of information provided by the comet assay with other DNA-damage indicators, and with cellular responses including oxidative stress, cell division or cell death. Finally, we will focus on putative relations between transcripts related with DNA damage pathways, DNA replication and repair, oxidative stress and cell cycle progression that have been identified in plant cells with comet assays demonstrating DNA damage.

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The comet assay in higher terrestrial plant model: Review and evolutionary trends

Lanier

Manier

Cuny

et al. 2015

Environmental Pollution

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Synergistic Cytotoxic Stress and DNA Damage in Clover (Trifolium repens) Exposed to Heavy Metal Soil from Automobile Refining Shops in Kashmir-Himalaya

Cited by 6 publications

References 26 publications

Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens

Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens

The use of comet assay in plant toxicology: recent advances

The comet assay in higher terrestrial plant model: Review and evolutionary trends

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